Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
BMC Biotechnology, 2011 Background MicroRNAs are important regulators of gene expression at the post-transcriptional level and play an important role in many biological processes.
Busk Peter K +2 more
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Development of PCR Blocking Primers Enabling DNA Metabarcoding Analysis of Dietary Composition in Hematophagous Sea Lamprey [PDF]
Ecology and EvolutionConventional dietary assessments are challenging in hematophagous species, particularly in sea lamprey (Petromyzon marinus). However, recent technological developments and molecular approaches have provided an attractive alternative through the use of ...
Conor O'Kane +5 more
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Subfamily-selective PCR primers for the human LINE1 L1PA lineage [PDF]
Scientific ReportsLong interspersed nuclear element-1 (LINE1) retrotransposons are classified into different subfamilies based on evolutionary history. For human biology, the L1PA lineage of LINE1s is particularly significant. This lineage contains both retrotransposition-
Marcel Misak +2 more
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Usefulness of microsatellite loci for differentiating between Dibothriocephalus dendriticus and Dibothriocephalus ditremus (Cestoda: Diphyllobothriidea) [PDF]
ParasiteDifferentiating between two diphyllobothriid tapeworms Dibothriocephalus dendriticus and Dibothriocephalus ditremus is complicated due to their morphological plasticity, intraspecific variability and a wide range of common hosts.
Králová-Hromadová Ivica +5 more
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Indonesian Journal of Halal Research, 2022 Adulterating meat products with several species, including non-halal species, is often found in commercial products. This study aims to design and validate the Cytochrome c oxidase subunit I (CO1) primers to detect the non-halal species.
Joni Kusnadi +5 more
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Allele-Specific PCR for PIK3CA Mutation Detection Using Phosphoryl Guanidine Modified Primers
Diagnostics, 2023 Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to increased discrimination between wild-type and mutated DNA, providing extraordinary ...
Alexey S. Chubarov +5 more
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Comparing the performance of 12S mitochondrial primers for fish environmental DNA across ecosystems
Environmental DNA, 2021 Through the development of environmental DNA (eDNA) metabarcoding, in situ monitoring of organisms is becoming easier and promises a revolution in our approaches to detect changes in biodiversity over space and time. A cornerstone of eDNA approach is the
A. Polanco F. +12 more
semanticscholar +1 more source
Improved efficiency and robustness in qPCR and multiplex end-point PCR by twisted intercalating nucleic acid modified primers. [PDF]
PLoS ONE, 2012 We introduce quantitative polymerase chain reaction (qPCR) primers and multiplex end-point PCR primers modified by the addition of a single ortho-Twisted Intercalating Nucleic Acid (o-TINA) molecule at the 5'-end.
Uffe Vest Schneider +5 more
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ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations [PDF]
BIO Web of Conferences, 2021 Using the Diamond DNA kit, high quality nuclear DNA was isolated from dry leaves of the endemic species Scutellaria tuvensis. The selection of primers for ISSR analysis of genetic polymorphism of natural populations is described.
Muraseva Dinara S., Guseva Alexandra A.
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Zanco Journal of Pure and Applied Sciences, 2020 The aim of present study to determine DNA polymorphism, between and within two different color (white) and (black) guinea fowl lines. Blood samples were collected about 3 ml of 30 white guinea fowl and 30 black guinea fowl, in six different local farms.
Hurea S. Abdulrazaq
doaj +3 more sources