Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs [PDF]
Frontiers in Zoology, 2008 Background Identification of DNA sequence diversity is a powerful means for assessing the species present in environmental samples. The most common molecular strategies for estimating taxonomic composition depend upon PCR with universal primers that ...
Jarman Simon N, Vestheim Hege
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DNA Amplification Fingerprinting Using Two Long Primers [PDF]
BioTechniques, 2001 Araceli Díaz-Perales +2 more
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Universal Primers for Sequencing DNA Restriction Fragments [PDF]
BioTechniques, 1997 Yeeru J. Chen, Manuel S. Valenzuela
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Indonesian Journal of Halal Research, 2022 Adulterating meat products with several species, including non-halal species, is often found in commercial products. This study aims to design and validate the Cytochrome c oxidase subunit I (CO1) primers to detect the non-halal species.
Joni Kusnadi +5 more
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Improved efficiency and robustness in qPCR and multiplex end-point PCR by twisted intercalating nucleic acid modified primers. [PDF]
PLoS ONE, 2012 We introduce quantitative polymerase chain reaction (qPCR) primers and multiplex end-point PCR primers modified by the addition of a single ortho-Twisted Intercalating Nucleic Acid (o-TINA) molecule at the 5'-end.
Uffe Vest Schneider +5 more
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Allele-Specific PCR for PIK3CA Mutation Detection Using Phosphoryl Guanidine Modified Primers
Diagnostics, 2023 Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to increased discrimination between wild-type and mutated DNA, providing extraordinary ...
Alexey S. Chubarov +5 more
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DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus [PDF]
, 1995 We used three short repetitive nucleotide sequences [(GTG)5, (TAC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted Citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic ...
Asif, M +7 more
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ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations [PDF]
BIO Web of Conferences, 2021 Using the Diamond DNA kit, high quality nuclear DNA was isolated from dry leaves of the endemic species Scutellaria tuvensis. The selection of primers for ISSR analysis of genetic polymorphism of natural populations is described.
Muraseva Dinara S., Guseva Alexandra A.
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Zanco Journal of Pure and Applied Sciences, 2020 The aim of present study to determine DNA polymorphism, between and within two different color (white) and (black) guinea fowl lines. Blood samples were collected about 3 ml of 30 white guinea fowl and 30 black guinea fowl, in six different local farms.
Hurea S. Abdulrazaq
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Comparing Microsporidia-targeting primers for environmental DNA sequencing
Parasite, 2023 Metabarcoding is a powerful tool to detect classical, and well-known “long-branch” Microsporidia in environmental samples. Several primer pairs were developed to target these unique microbial parasites, the majority of which remain undetected when using ...
Doliwa Annemie +3 more
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