Results 11 to 20 of about 3,903,497 (411)

High-Coverage ITS Primers for the DNA-Based Identification of Ascomycetes and Basidiomycetes in Environmental Samples

open access: yesPLoS ONE, 2012
The kingdom Fungi is estimated to include 1.5 million or more species, playing key roles as decomposers, mutualists, and parasites in every biome on the earth.
Hirokazu Toju   +2 more
exaly   +2 more sources

Development of environmental DNA metabarcoding primers for marine mollusks and comparison with published primers

open access: yesBMC Ecology and Evolution
Monitoring mollusk biodiversity is a great challenge due to their large diversity and broad distribution. Environmental DNA (eDNA) technology is increasingly applied for biodiversity monitoring, but relevant studies on marine mollusks are still limited ...
Xiaojing Shi   +3 more
doaj   +3 more sources

Design and Performance Test of Specific Primers to Detect Bovine DNA Fragments using Multiplex PCR Technique for Halal Authentification

open access: yesIndonesian Journal of Halal Research, 2022
Adulterating meat products with several species, including non-halal species, is often found in commercial products. This study aims to design and validate the Cytochrome c oxidase subunit I (CO1) primers to detect the non-halal species.
Joni Kusnadi   +5 more
doaj   +1 more source

Allele-Specific PCR for PIK3CA Mutation Detection Using Phosphoryl Guanidine Modified Primers

open access: yesDiagnostics, 2023
Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to increased discrimination between wild-type and mutated DNA, providing extraordinary ...
Alexey S. Chubarov   +5 more
doaj   +1 more source

Comparing the performance of 12S mitochondrial primers for fish environmental DNA across ecosystems

open access: yesEnvironmental DNA, 2021
Through the development of environmental DNA (eDNA) metabarcoding, in situ monitoring of organisms is becoming easier and promises a revolution in our approaches to detect changes in biodiversity over space and time. A cornerstone of eDNA approach is the
A. Polanco F.   +12 more
semanticscholar   +1 more source

DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus [PDF]

open access: yes, 1995
We used three short repetitive nucleotide sequences [(GTG)5, (TAC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted Citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic ...
Asif, M   +7 more
core   +3 more sources

Genetic Similarity of two different color Local Guinea Fowl (Numida Meleagris) By Using RAPD-PCR Technique

open access: yesZanco Journal of Pure and Applied Sciences, 2020
The aim of present study to determine DNA polymorphism, between and within two different color (white) and (black) guinea fowl lines. Blood samples were collected about 3 ml of 30 white guinea fowl and 30 black guinea fowl, in six different local farms.
Hurea S. Abdulrazaq
doaj   +3 more sources

ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations [PDF]

open access: yesBIO Web of Conferences, 2021
Using the Diamond DNA kit, high quality nuclear DNA was isolated from dry leaves of the endemic species Scutellaria tuvensis. The selection of primers for ISSR analysis of genetic polymorphism of natural populations is described.
Muraseva Dinara S., Guseva Alexandra A.
doaj   +1 more source

DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

open access: yesNucleic Acids Research, 1990
Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross.
John G. K. Williams   +6 more
semanticscholar   +1 more source

Home - About - Disclaimer - Privacy