Results 21 to 30 of about 3,489,226 (291)

New universal ITS2 primers for high-resolution herbivory analyses using DNA metabarcoding in both tropical and temperate zones

open access: yesScientific Reports, 2018
DNA metabarcoding is a rapidly growing technique for obtaining detailed dietary information. Current metabarcoding methods for herbivory, using a single locus, can lack taxonomic resolution for some applications.
Rosemary J. Moorhouse‐Gann   +6 more
semanticscholar   +1 more source

Studies on the minimal lengths required for DNA primers to be extended by the Tetrahymena telomerase: implications for primer positioning by the enzyme.

open access: yesNucleic Acids Research, 2002
Telomerase is a specialized reverse transcriptase that contains an integral RNA subunit including a short template sequence. It extends telomeric 3' overhangs and chromosome breakpoints by catalyzing reiterative copying of this internal template into ...
N. Baran, Y. Haviv, Beena Paul, H. Manor
semanticscholar   +1 more source

Microflora ecology of the chicken intestine using 16S ribosomal DNA primers.

open access: yesPoultry Science, 2004
The microflora in the gastrointestinal tract of broiler chickens influences digestion, health, and wellbeing. Analysis of chicken gut microflora has been mainly by culture-based methods.
E. Amit-Romach, D. Sklan, Z. Uni
semanticscholar   +1 more source

PCR primers for metazoan nuclear 18S and 28S ribosomal DNA sequences. [PDF]

open access: yesPLoS ONE, 2012
BACKGROUND: Metagenetic analyses, which amplify and sequence target marker DNA regions from environmental samples, are increasingly employed to assess the biodiversity of communities of small organisms. Using this approach, our understanding of microbial
Ryuji J Machida, Nancy Knowlton
doaj   +1 more source

Comparison of different primes for PCR-based diagnosis of cutaneous leishmaniasis

open access: yesBrazilian Journal of Infectious Diseases
OBJECTIVE: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous ...
Diego Molina de Oliveira   +3 more
doaj   +1 more source

FoxO1 signaling in B cell malignancies and its therapeutic targeting

open access: yesFEBS Letters, EarlyView.
FoxO1 has context‐specific tumor suppressor or oncogenic character in myeloid and B cell malignancies. This includes tumor‐promoting properties such as stemness maintenance and DNA damage tolerance in acute leukemias, or regulation of cell proliferation and survival, or migration in mature B cell malignancies.
Krystof Hlavac   +3 more
wiley   +1 more source

STUDI PERBANDINGAN PEMAKAIAN PRIMER HBS1 DAN HBS2 DENGAN PRIMER P7 DAN P8 DALAM DETEKSI DNA VIRUS HEPATITIS B PADA PENDONOR DARAH

open access: yesFlorea: Jurnal Biologi dan Pembelajarannya, 2020
Examination of the hepatitis B virus as a disease of hepatitis serologically has been carried out a lot, but this serological examination can experience problems if there is a low window period, so even though a negative test result is still possible in ...
Supiana Dian Nurtjahyani   +2 more
doaj   +1 more source

Insights into PI3K/AKT signaling in B cell development and chronic lymphocytic leukemia

open access: yesFEBS Letters, EarlyView.
This Review explores how the phosphoinositide 3‐kinase and protein kinase B pathway shapes B cell development and drives chronic lymphocytic leukemia, a common blood cancer. It examines how signaling levels affect disease progression, addresses treatment challenges, and introduces novel experimental strategies to improve therapies and patient outcomes.
Maike Buchner
wiley   +1 more source

Pilot progress in DNA isolation and amplification from the material stored at the LWS herbarium

open access: yesІнтродукція Рослин
The isolation of DNA from the herbarium specimens deposited at the LWS herbarium (State Museum of Natural History of the NAS of Ukraine, Lviv, Ukraine) has been tested using the column-based protocol.
Andriy Novikov, Viktor Nachychko
doaj   +1 more source

Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs

open access: yesFrontiers in Zoology, 2008
Background Identification of DNA sequence diversity is a powerful means for assessing the species present in environmental samples. The most common molecular strategies for estimating taxonomic composition depend upon PCR with universal primers that ...
Jarman Simon N, Vestheim Hege
doaj   +1 more source

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