Results 21 to 30 of about 3,903,497 (411)

Using long ssDNA polynucleotides to amplify STRs loci in degraded DNA samples. [PDF]

PLoS ONE, 2017
Obtaining informative short tandem repeat (STR) profiles from degraded DNA samples is a challenging task usually undermined by locus or allele dropouts and peak-high imbalances observed in capillary electrophoresis (CE) electropherograms, especially for ...
Martín E Mautner, Agustín Pérez Santángelo, Rodrigo M Corti Bielsa, Andrea Sala, Santiago Ginart, Daniel Corach   +5 more
doaj   +1 more source

Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]

, 1992
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke, AHN Hopman, AR Mitchell, C Huxley, C Lengauer, Christoph Lengauer, D Pinkel, ED Green, EW Jabs, HF Willard, HF Willard, HF Willard, HM Rosenberg, Ian Dunham, J Anastasi, J Meyne, JE Koch, JS Waye, JS Waye, JS Waye, L Manuelidis, L Manuelidis, M Klever, P Emmerich, PJ Poddighe, PR Langer, S Popp, T Cremer, T Cremer, T Cremer, T Imai, T Maniatis, Terry Featherstone, Thomas Cremer   +33 more
core   +1 more source

High-throughput sequencing of 16S rRNA gene amplicons : effects of extraction procedure, primer length and annealing temperature [PDF]

, 2012
The analysis of 16S-rDNA sequences to assess the bacterial community composition of a sample is a widely used technique that has increased with the advent of high throughput sequencing.
Cogan, Tristan, Constantinidou, Chrystala, Pallen, Mark J., Penn, Charles W., Sergeant, Martin J.   +4 more
core   +3 more sources

Stringent Primer Termination by an Archaeo-Eukaryotic DNA Primase [PDF]

Frontiers in Microbiology, 2021
Priming of single stranded templates is essential for DNA replication. In recent years, significant progress was made in understanding how DNA primase fulfils this fundamental function, particularly with regard to the initiation. Equally intriguing is the unique property of archeao-eukaryotic primases to terminate primer formation at a well-defined ...
Jan Bergsch, Jan Bergsch, Jean-Christophe Devillier, Jean-Christophe Devillier, Gunnar Jeschke, Georg Lipps   +5 more
openaire   +5 more sources

Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers.

Proceedings of the National Academy of Sciences of the United States of America, 1989
With a standard set of primers directed toward conserved regions, we have used the polymerase chain reaction to amplify homologous segments of mtDNA from more than 100 animal species, including mammals, birds, amphibians, fishes, and some invertebrates ...
T. Kocher, W. Thomas, A. Meyer, S. Edwards, S. Pääbo, F. Villablanca, A. Wilson   +6 more
semanticscholar   +1 more source

Specific Detection of Leuconostoc mesenteroides subsp. mesenteroides with DNA Primers Identified by Randomly Amplified Polymorphic DNA Analysis

Applied and Environmental Microbiology, 2000
Giancarlo Moschetti, Giuseppe Blaiotta, Francesco Villani, Salvatore Coppola   +3 more
openalex   +2 more sources

Tracing the Pig and Cattle Origin in Processed Food and Feed Products Targeting Mitochondrial 12S rRNA Gene

Journal of Food Quality and Hazards Control, 2021
Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using
D.C. Roy, S. Akhter, A.K. Sarker, M.M.K. Hossain, C. Lyzu, L.C. Mohanta, D. Islam, M.A.A. Khan   +7 more
doaj  

Reliable and robust molecular sexing of the hen harrier (Circus cyaneus) using PCR-RFLP of the CHD1 gene [PDF]

, 2013
The hen harrier (Circus cyaneus) is a bird of prey that is persecuted in the United Kingdom, and there is a need for a DNA-based individual identification and sexing system for the use in forensic investigations.
Henderson, A, Iyengar, Arati, Lee, C, Mistry, V, Thomas, M   +4 more
core   +1 more source

Uji Deteksi Adulterasi Daging Babi (Sus scrofa domestica) pada Bakso Metode Loop-Mediated Isothermal Amplification

Indonesian Journal of Human Nutrition, 2021
Composition adulteration in a product is still a serious problem. For that we need a DNA-based test method that is fast, specific and simple. In this study, a rapid test technique with the loop-mediated isothermal amplification method was applied to ...
Rahma Micho Widyanto, Cleonara Yanuar Dini, Irma Sarita Rahmawati, Sekar Ramadhanti Putri, Amelia Nurdini Rozana, Sakinah Hilya Abida, Yunimar Yunimar   +6 more
doaj   +1 more source

Off-lattice simulation of the solid phase DNA amplification [PDF]

Comp. Phys. Commun. 170 (2005) 131, 2004
Recent simulations of the solid phase DNA amplification (SPA) by J.-F. Mercier et al (Biophys. J. 85 (2003) 2075) are generalized to include two kinds of primers and the off-lattice character of the primer distribution on the surface. The sigmoidal character of the primer occupation by DNA, observed experimentally, is reproduced in the simulation.
arxiv   +1 more source