Results 21 to 30 of about 4,042,809 (333)
Using long ssDNA polynucleotides to amplify STRs loci in degraded DNA samples. [PDF]
PLoS ONE, 2017 Obtaining informative short tandem repeat (STR) profiles from degraded DNA samples is a challenging task usually undermined by locus or allele dropouts and peak-high imbalances observed in capillary electrophoresis (CE) electropherograms, especially for ...
Martín E Mautner +5 more
doaj +1 more source
Ten polymorphic microsatellite primers in the tropical tree caimito, Chrysophyllum cainito (Sapotaceae). [PDF]
, 2014 UnlabelledPremise of the studyWe developed microsatellite primers for the tropical tree Chrysophyllum cainito (Sapotaceae) to determine the native range of the species, investigate the origin of cultivated populations, and examine the partitioning of ...
Parker, Ingrid M +2 more
core +3 more sources
Indonesian Journal of Human Nutrition, 2021 Composition adulteration in a product is still a serious problem. For that we need a DNA-based test method that is fast, specific and simple. In this study, a rapid test technique with the loop-mediated isothermal amplification method was applied to ...
Rahma Micho Widyanto +6 more
doaj +1 more source
PlantID – DNA-based identification of multiple medicinal plants in complex mixtures [PDF]
, 2012 Background An efficient method for the identification of medicinal plant products is now a priority as the global demand increases. This study aims to develop a DNA-based method for the identification and authentication of plant species that can be ...
Bremner, Paul +7 more
core +2 more sources
Scientific Reports, 2023 16S rRNA amplicon sequencing or, more recently, metatranscriptomic analysis are currently the only preferred methods for microbial profiling of samples containing a predominant ratio of human to bacterial DNA. However, due to the off-target amplification
Tereza Deissová +7 more
doaj +1 more source
Molecular Ecology, 2018 DNA metabarcoding is a technique used to survey biodiversity in many ecological settings, but there are doubts about whether it can provide quantitative results, that is, the proportions of each species in the mixture as opposed to a species list.
J. Piñol, M. A. Senar, W. Symondson
semanticscholar +1 more source
Nanoparticle labels for pathogen detection through nucleic acid amplification tests [PDF]
, 2014 YesMagnetic nanoparticles and surface-enhanced Raman scattering (SERS) active nanoparticles were coated with short chain DNA tags. These were then used to identify a target bacterial DNA sequence.
Chen, Y-C. +3 more
core +1 more source
Evaluating detection limits of next-generation sequencing for the surveillance and monitoring of international marine pests [PDF]
, 2013 Most surveillance programmes for marine invasive species (MIS) require considerable taxonomic expertise, are laborious, and are unable to identify species at larval or juvenile stages.
Bott, Nathan J. +3 more
core +14 more sources
Accidental Amplification of the Avian Influenza N4 Gene Segment by N1-Specific Primers
مجلة الانبار للعلوم البيطريةBackground: Avian influenza is a worldwide animal and public health risk. With increasing bird-to-human transmission, it has become a significant infectious agent.
Khlef, Evan Latef
doaj +1 more source
Standard and Light-Cycler PCR methods for animal DNA species detection in animal feedstuffs [PDF]
, 2008 In this work four species-specific primers and probes were designed and evaluated for the detection and quantification of bovine, ovine, swine and chicken mitochondrial DNA in feeds.
Ajmone_Marsan, P +12 more
core +1 more source