Results 31 to 40 of about 3,489,226 (291)
F1000Research, 2021 The prevalence of Herpes simplex virus 2 (HSV2) is increasing at an alarming rate in the world. Most of the HSV2 cases are not diagnosed properly, although a range of molecular and serological diagnoses exist.
Modhusudon Shaha +2 more
doaj +1 more source
Self-reporting PNA/DNA primers for PCR analysis.
Genome Research, 2001 We report a new fluorogenic method for sealed-tube PCR analysis using a quencher-labeled peptide nucleic acid (Q-PNA) probe. The Q-PNA hybridizes to a complementary tag sequence located at the 5' end of a 5' fluorophore-labeled oligonucleotide primer ...
M. Fiandaca +3 more
semanticscholar +1 more source
Making tau amyloid models in vitro: a crucial and underestimated challenge
FEBS Letters, EarlyView.This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley +1 more source
FEBS Letters, EarlyView.B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro +2 more
wiley +1 more source
Evolutionary interplay between viruses and R‐loops
FEBS Letters, EarlyView.Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi +4 more
wiley +1 more source
DiversityEnvironmental DNA from bulk materials can be analyzed to gain an understanding of the bacterial, fungal, plant, and/or arthropod communities present. DNA metabarcoding is widely used to characterize these biological communities, by amplifying “barcode ...
Teresa M. Tiedge +2 more
doaj +1 more source
Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts
BMC Microbiology, 2005 Background The Internal Transcribed Spacer (ITS) regions of fungal ribosomal DNA (rDNA) are highly variable sequences of great importance in distinguishing fungal species by PCR analysis.
Rygiewicz Paul T, Martin Kendall J
doaj +1 more source
Circulating histones as clinical biomarkers in critically ill conditions
FEBS Letters, EarlyView.Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez +17 more
wiley +1 more source
Detection of Leaf Blotches - Causal Agents in Barley Leaves and Grains
Czech Journal of Genetics and Plant Breeding, 2004 The objective of this paper was to adapt PCR-based detection method for R. secalis and P. teres DNA isolated from pathogens and also from artificially infected juvenile leaves and seeds using pathogen-specific primers.
J. Gubiš +5 more
doaj +1 more source
Identifi cation of Salmonella spp and serovars Typhimurium, Enteritidis by qPCR
Науковий вісник ветеринарної медицини, 2020 This article presents the results of the identifi cation of the Salmonella genus as well as serovars Enteritidis and Typhimurium by a real-time polymerase chain reaction.
Rublenko N.
doaj +1 more source