Results 31 to 40 of about 3,903,497 (411)
Performance of a TthPrimPol-based whole genome amplification kit for copy number alteration detection using massively parallel sequencing [PDF]
, 2016 Starting from only a few cells, current whole genome amplification (WGA) methods provide enough DNA to perform massively parallel sequencing (MPS). Unfortunately, all current WGA methods introduce representation bias which limits detection of copy number
De Coninck, Dieter +6 more
core +1 more source
Molecular Ecology, 2018 DNA metabarcoding is a technique used to survey biodiversity in many ecological settings, but there are doubts about whether it can provide quantitative results, that is, the proportions of each species in the mixture as opposed to a species list.
J. Piñol, M. A. Senar, W. Symondson
semanticscholar +1 more source
Scientific Reports, 2023 16S rRNA amplicon sequencing or, more recently, metatranscriptomic analysis are currently the only preferred methods for microbial profiling of samples containing a predominant ratio of human to bacterial DNA. However, due to the off-target amplification
Tereza Deissová +7 more
doaj +1 more source
A primer on the oxDNA model of DNA: When to use it, how to simulate it and how to interpret the results [PDF]
Front. Mol. Biosci. 8, 693710 (2021), 2021 The oxDNA model of DNA has been applied widely to systems in biology, biophysics and nanotechnology. It is currently available via two independent open source packages. Here we present a set of clearly-documented exemplar simulations that simultaneously provide both an introduction to simulating the model, and a review of the model's fundamental ...
arxiv +1 more source
16S rRNA gene sequencing of mock microbial populations- impact of DNA extraction method, primer choice and sequencing platform [PDF]
, 2016 peer-reviewedBackground Next-generation sequencing platforms have revolutionised our ability to investigate the microbiota composition of complex environments, frequently through 16S rRNA gene sequencing of the bacterial component of the
Claesson, Marcus J. +4 more
core +2 more sources
Royal Society Open Science, 2015 We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned whole mitochondrial genome (mitogenome) sequences from 880 species, supplemented by partial mitogenome ...
M. Miya +11 more
semanticscholar +1 more source
An efficient method for DNA extraction from Cladosporioid fungi [PDF]
, 2010 We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A. +3 more
core +2 more sources
Scientific Reports, 2018 DNA metabarcoding is a rapidly growing technique for obtaining detailed dietary information. Current metabarcoding methods for herbivory, using a single locus, can lack taxonomic resolution for some applications.
Rosemary J Moorhouse-Gann +6 more
semanticscholar +1 more source
Ten polymorphic microsatellite primers in the tropical tree caimito, Chrysophyllum cainito (Sapotaceae). [PDF]
, 2014 UnlabelledPremise of the studyWe developed microsatellite primers for the tropical tree Chrysophyllum cainito (Sapotaceae) to determine the native range of the species, investigate the origin of cultivated populations, and examine the partitioning of ...
Parker, Ingrid M +2 more
core +3 more sources
Nucleic Acids Research, 1985 A rapid and versatile method has been developed for the synthesis of oligonucleotides which contain an aliphatic amino group at their 5' terminus. This amino group reacts specifically with a variety of electrophiles, thereby allowing other chemical ...
Lloyd M. Smith +4 more
semanticscholar +1 more source