Self-reporting PNA/DNA primers for PCR analysis.
We report a new fluorogenic method for sealed-tube PCR analysis using a quencher-labeled peptide nucleic acid (Q-PNA) probe. The Q-PNA hybridizes to a complementary tag sequence located at the 5' end of a 5' fluorophore-labeled oligonucleotide primer ...
M. Fiandaca+3 more
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Pilot progress in DNA isolation and amplification from the material stored at the LWS herbarium
The isolation of DNA from the herbarium specimens deposited at the LWS herbarium (State Museum of Natural History of the NAS of Ukraine, Lviv, Ukraine) has been tested using the column-based protocol.
Andriy Novikov, Viktor Nachychko
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Background Identification of DNA sequence diversity is a powerful means for assessing the species present in environmental samples. The most common molecular strategies for estimating taxonomic composition depend upon PCR with universal primers that ...
Jarman Simon N, Vestheim Hege
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Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts
Background The Internal Transcribed Spacer (ITS) regions of fungal ribosomal DNA (rDNA) are highly variable sequences of great importance in distinguishing fungal species by PCR analysis.
Rygiewicz Paul T, Martin Kendall J
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Environmental DNA from bulk materials can be analyzed to gain an understanding of the bacterial, fungal, plant, and/or arthropod communities present. DNA metabarcoding is widely used to characterize these biological communities, by amplifying “barcode ...
Teresa M. Tiedge+2 more
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Identifi cation of Salmonella spp and serovars Typhimurium, Enteritidis by qPCR
This article presents the results of the identifi cation of the Salmonella genus as well as serovars Enteritidis and Typhimurium by a real-time polymerase chain reaction.
Rublenko N.
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Detection of Leaf Blotches - Causal Agents in Barley Leaves and Grains
The objective of this paper was to adapt PCR-based detection method for R. secalis and P. teres DNA isolated from pathogens and also from artificially infected juvenile leaves and seeds using pathogen-specific primers.
J. Gubiš+5 more
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Development of a Species Diagnostic Molecular Tool for an Invasive Pest, Mythimna loreyi, Using LAMP
The Mythimna loreyi (Duponchel) is one of the well-known invasive noctuid pests in Africa, Australia, and many Asian countries. However, it is difficult to identify the invasive and morphologically similar species, Mythimna separate, which occur at the ...
Hwa Yeun Nam+3 more
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Comparison of different primes for PCR-based diagnosis of cutaneous leishmaniasis
Objective: The objective of this study was to analyze different primers that are commonly used in epidemiological studies for the detection of Leishmania DNA by PCR, and to compare them to the conventional direct parasite search for American cutaneous ...
Diego Molina de Oliveira, M.D.+3 more
doaj
Effectiveness of annealing blocking primers versus restriction enzymes for characterization of generalist diets: unexpected prey revealed in the gut contents of two coral reef fish species. [PDF]
Characterization of predator-prey interactions is challenging as researchers have to rely on indirect methods that can be costly, biased and too imprecise to elucidate the complexity of food webs.
Matthieu Leray+3 more
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