Primer initiation and extension by T7 DNA primase [PDF]
The EMBO Journal, 2006 T7 DNA primase is composed of a catalytic RNA polymerase domain (RPD) and a zinc-binding domain (ZBD) connected by an unstructured linker. The two domains are required to initiate the synthesis of the diribonucleotide pppAC and its extension into a functional primer pppACCC (de novo synthesis), as well as for the extension of exogenous AC ...
Seung-Joo Lee +3 more
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PCR primers for metazoan nuclear 18S and 28S ribosomal DNA sequences. [PDF]
PLoS ONE, 2012 BACKGROUND: Metagenetic analyses, which amplify and sequence target marker DNA regions from environmental samples, are increasingly employed to assess the biodiversity of communities of small organisms. Using this approach, our understanding of microbial
Ryuji J Machida, Nancy Knowlton
doaj +1 more source
Polymerase chain reactions with alphoid-repeat primers in combination with Alu or LINEs primers, generate chromosome-specific DNA fragments [PDF]
, 1991 Y alphoid primers in combination with Alu and LINEs primers generated new DNA fragments in polymerase chain reactions (PCR) on DNA from a Y-only somatic cell hybrid but not from X-only, 3-only, or 21-only hybrids.
Erickson, Robert P. +3 more
core +1 more source
Microflora ecology of the chicken intestine using 16S ribosomal DNA primers.
Poultry Science, 2004 The microflora in the gastrointestinal tract of broiler chickens influences digestion, health, and wellbeing. Analysis of chicken gut microflora has been mainly by culture-based methods.
E. Amit-Romach, D. Sklan, Z. Uni
semanticscholar +1 more source
Computational and Structural Biotechnology Journal, 2023 Polymerase chain reaction (PCR) is a powerful molecular biology assay for gene detection and quantification. Conventional DNA primers for PCR often suffer from poor sensitivity in specific gene detection.
Yi-Chen Tsai +2 more
doaj
External quality assessment of molecular biology-based methods used in laboratories of clinical chemistry and human genetics [PDF]
, 1998 The Reference Institute of Bioanalysis of the German Society of Clinical Chemistry has performed the first external assessment of molecular genetics methods used in medical diagnosis.
Braun, Andreas +6 more
core +1 more source
Primer Extension Analysis of HBV DNA with Strand-Specific Primers
BIO-PROTOCOL, 2015 We performed primer extension assay to determine which steps of HBV DNA synthesis (i.e., minus- and plus-strand DNA synthesis and circularization of RC DNA) are affected by phosphoacceptor site mutations in C protein. In these experiments, we used several specific oligonucleotide primers.
Kyongmin Kim, Jaesung Jung
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Transduction of DNA information through water and electromagnetic waves [PDF]
Electromagnetic Biology and Medicine 2015.34:106-112, 2014 The experimental conditions by which electromagnetic signals (EMS) of low frequency can be emitted by diluted aqueous solutions of some bacterial and viral DNAs are described. That the recorded EMS and nanostructures induced in water carry the DNA information (sequence) is shown by retrieval of that same DNA by classical PCR amplification using the TAQ
arxiv +1 more source
A noninvasive molecular approach: exploiting species-locus-specific PCR primers in defeating numts and DNA cross-contamination of cercopithecidae [PDF]
, 2017 The lack of a standardized, noninvasive molecular approach to studying genetic aspects of primates has made it hard for primatologists to decode the evolutionary history of these species.
Abu Bakar, Muhammad Abdul Latiff +3 more
core
Amplifying DNA with arbitrary oligonucleotide primers. [PDF]
Genome Research, 1993 G e n e t i c molecular markers are DNA segments that behave as landmarks for genome analysis. These segments usually represent variant or polymorphic sites that can be identified using general strategies such as molecular hybridization or enzymatic amplification of DNA.
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