Results 1 to 10 of about 634,273 (149)

Temporal control of human DNA replication licensing by CDK4/6-RB signalling and chemical genetics. [PDF]

Nat Commun
Sosenko Piscitello A, Nilsson AS, Hawgood M, Sayyid AH, Dionellis VS, Giglio G, Urién B, Bajgain P, Ntallis SG, Bartek J, Halazonetis TD, Lemmens B.   +11 more
europepmc   +3 more sources

MTBP phosphorylation controls DNA replication origin firing

Scientific Reports, 2021
Faithful genome duplication requires regulation of origin firing to determine loci, timing and efficiency of replisome generation. Established kinase targets for eukaryotic origin firing regulation are the Mcm2-7 helicase, Sld3/Treslin/TICRR and Sld2 ...
Pedro Ferreira, Verena Höfer, Nora Kronshage, Anika Marko, Karl-Uwe Reusswig, Bilal Tetik, Christoph Dießel, Kerstin Köhler, Nikolai Tschernoster, Janine Altmüller, Nina Schulze, Boris Pfander, Dominik Boos   +12 more
doaj   +1 more source

The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer

Nature Communications, 2022
Here the authors describe multiple structures of the replicative helicase MCM2-7 in complex with Dbf4-dependent kinase (DDK). These structures reveal why the kinase specifically recognizes the MCM2-7 double-hexamer over the single-hexamer and explain how
Almutasem Saleh, Yasunori Noguchi, Ricardo Aramayo, Marina E. Ivanova, Kathryn M. Stevens, Alex Montoya, S. Sunidhi, Nicolas Lopez Carranza, Marcin J. Skwark, Christian Speck   +9 more
doaj   +1 more source

The Contribution of Lysosomes to DNA Replication

Cells, 2021
Lysosomes, acidic, membrane-bound organelles, are not only the core of the cellular recycling machinery, but they also serve as signaling hubs regulating various metabolic pathways. Lysosomes maintain energy homeostasis and provide pivotal substrates for
Joanna Maria Merchut-Maya, Apolinar Maya-Mendoza   +1 more
doaj   +1 more source

Cryo-EM structures reveal that RFC recognizes both the 3′- and 5′-DNA ends to load PCNA onto gaps for DNA repair

eLife, 2022
RFC uses ATP to assemble PCNA onto primed sites for replicative DNA polymerases δ and ε. The RFC pentamer forms a central chamber that binds 3′ ss/ds DNA junctions to load PCNA onto DNA during replication.
Fengwei Zheng, Roxana Georgescu, Nina Y Yao, Huilin Li, Michael E O'Donnell   +4 more
doaj   +1 more source

The structure of ORC–Cdc6 on an origin DNA reveals the mechanism of ORC activation by the replication initiator Cdc6

Nature Communications, 2021
Eukaryotic DNA replication is mediated by many proteins which are tightly regulated for an efficient firing of replication at each cell cycle. Here the authors report a cryo-EM structure of the yeast ORC–Cdc6 bound to an 85-bp ARS1 origin DNA revealing ...
Xiang Feng, Yasunori Noguchi, Marta Barbon, Bruce Stillman, Christian Speck, Huilin Li   +5 more
doaj   +1 more source

Structures of 9-1-1 DNA checkpoint clamp loading at gaps from start to finish and ramification on biology

Cell Reports, 2023
Summary: Rad24-RFC (replication factor C) loads the 9-1-1 checkpoint clamp onto the recessed 5′ ends by binding a 5′ DNA at an external surface site and threading the 3′ single-stranded DNA (ssDNA) into 9-1-1. We find here that Rad24-RFC loads 9-1-1 onto
Fengwei Zheng, Roxana E. Georgescu, Nina Y. Yao, Michael E. O’Donnell, Huilin Li   +4 more
doaj   +1 more source

An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases

eLife, 2020
Cell cycle tags allow to restrict target protein expression to specific cell cycle phases. Here, we present an advanced toolbox of cell cycle tag constructs in budding yeast with defined and compatible peak expression that allow comparison of protein ...
Julia Bittmann, Rokas Grigaitis, Lorenzo Galanti, Silas Amarell, Florian Wilfling, Joao Matos, Boris Pfander   +6 more
doaj   +1 more source

Unscheduled DNA replication in G1 causes genome instability and damage signatures indicative of replication collisions

Nature Communications, 2022
Reusswig et al. use engineered systems to force DNA replication in the G1 phase of the cell cycle. This unscheduled G1 replication shows hallmarks of S phase replication, but leads to over-replication and DNA breaks from replication collisions.
Karl-Uwe Reusswig, Julia Bittmann, Martina Peritore, Mathilde Courtes, Benjamin Pardo, Michael Wierer, Matthias Mann, Boris Pfander   +7 more
doaj   +1 more source

Molecular choreography of primer synthesis by the eukaryotic Pol α-primase

Nature Communications, 2023
The eukaryotic polymerase α (Pol α) synthesizes an RNA-DNA hybrid primer of 20–30 nucleotides. Pol α is composed of Pol1, Pol12, Primase 1 (Pri1), and Pri2. Pol1 and Pri1 contain the DNA polymerase and RNA primase activities, respectively.
Zuanning Yuan, Roxana Georgescu, Huilin Li, Michael E. O’Donnell   +3 more
doaj   +1 more source