Results 1 to 10 of about 1,048,115 (189)
MTBP phosphorylation controls DNA replication origin firing
Scientific Reports, 2021 Faithful genome duplication requires regulation of origin firing to determine loci, timing and efficiency of replisome generation. Established kinase targets for eukaryotic origin firing regulation are the Mcm2-7 helicase, Sld3/Treslin/TICRR and Sld2 ...
Pedro Ferreira +12 more
doaj +1 more source
Nature Communications, 2022 Here the authors describe multiple structures of the replicative helicase MCM2-7 in complex with Dbf4-dependent kinase (DDK). These structures reveal why the kinase specifically recognizes the MCM2-7 double-hexamer over the single-hexamer and explain how
Almutasem Saleh +9 more
doaj +1 more source
Decreased MCM2-6 in Drosophila S2 cells does not generate significant DNA damage or cause a marked increase in sensitivity to replication interference. [PDF]
, 2011 A reduction in the level of some MCM proteins in human cancer cells (MCM5 in U20S cells or MCM3 in Hela cells) causes a rapid increase in the level of DNA damage under normal conditions of cell proliferation and a loss of viability when the cells are ...
A Ibarra +29 more
core +18 more sources
The Contribution of Lysosomes to DNA Replication
Cells, 2021 Lysosomes, acidic, membrane-bound organelles, are not only the core of the cellular recycling machinery, but they also serve as signaling hubs regulating various metabolic pathways. Lysosomes maintain energy homeostasis and provide pivotal substrates for
Joanna Maria Merchut-Maya +1 more
doaj +1 more source
Cohesin causes replicative DNA damage by trapping DNA topological stress [PDF]
, 2020 DNA topological stress inhibits DNA replication fork (RF) progression and contributes to DNA replication stress. In Saccharomyces cerevisiae, we demonstrate that centromeric DNA and the rDNA array are especially vulnerable to DNA topological stress ...
Baxter, Jonathan +2 more
core +1 more source
eLife, 2022 RFC uses ATP to assemble PCNA onto primed sites for replicative DNA polymerases δ and ε. The RFC pentamer forms a central chamber that binds 3′ ss/ds DNA junctions to load PCNA onto DNA during replication.
Fengwei Zheng +4 more
doaj +1 more source
Nature Communications, 2021 Eukaryotic DNA replication is mediated by many proteins which are tightly regulated for an efficient firing of replication at each cell cycle. Here the authors report a cryo-EM structure of the yeast ORC–Cdc6 bound to an 85-bp ARS1 origin DNA revealing ...
Xiang Feng +5 more
doaj +1 more source
Cell Reports, 2023 Summary: Rad24-RFC (replication factor C) loads the 9-1-1 checkpoint clamp onto the recessed 5′ ends by binding a 5′ DNA at an external surface site and threading the 3′ single-stranded DNA (ssDNA) into 9-1-1. We find here that Rad24-RFC loads 9-1-1 onto
Fengwei Zheng +4 more
doaj +1 more source
eLife, 2020 Cell cycle tags allow to restrict target protein expression to specific cell cycle phases. Here, we present an advanced toolbox of cell cycle tag constructs in budding yeast with defined and compatible peak expression that allow comparison of protein ...
Julia Bittmann +6 more
doaj +1 more source
Nature Communications, 2022 Reusswig et al. use engineered systems to force DNA replication in the G1 phase of the cell cycle. This unscheduled G1 replication shows hallmarks of S phase replication, but leads to over-replication and DNA breaks from replication collisions.
Karl-Uwe Reusswig +7 more
doaj +1 more source