Results 1 to 10 of about 2,461,779 (300)

DNA replication and beyond [PDF]

Nature Reviews Molecular Cell Biology, 2016
It turns out that Meselson and Stahl were not the first to show that DNA replicates semi-conservatively. The preceding experiment was almost forgotten, partly because it showed something incredible.
Joseph G. Gall
exaly   +3 more sources

Conservative DNA Replication [PDF]

Nature Reviews Molecular Cell Biology, 2014
Thanos D. Halazonetis describes the discovery that break-induced replication is conservative in budding yeast.
Thanos D Halazonetis
exaly   +5 more sources

ATP is dispensable for E. coli DNA replication and eukaryotic helicase activity [PDF]

Nature Communications
Adenosine triphosphate (ATP) hydrolysis is the main cellular source of energy used to drive biochemical reactions that are otherwise energetically unfavourable.
Richard R. Spinks, Aleksa Lakic, Celine Kelso, Slobodan Jergic, Olga Yurieva, Zhi-Qiang Xu, Michael E. O’Donnell, Nicholas E. Dixon, Antoine M. van Oijen, Jacob S. Lewis, Lisanne M. Spenkelink   +10 more
doaj   +2 more sources

DNA replication is required for the checkpoint response to damaged DNA in Xenopus egg extracts. [PDF]

, 2002
Alkylating agents, such as methyl methanesulfonate (MMS), damage DNA and activate the DNA damage checkpoint. Although many of the checkpoint proteins that transduce damage signals have been identified and characterized, the mechanism that senses the ...
Lindsay, Howard D., Stokes, Matthew P., Van Hatten, Ruth, Howard D. Lindsay (4252681), Howard D. Lindsay, W. Matthew Michael, Ruth Van Hatten (16043837), W. Matthew Michael (3211092), Ruth Van Hatten, Matthew P. Stokes, Matthew P. Stokes (9707794), Michael, W. Matthew   +11 more
core   +5 more sources

MTBP phosphorylation controls DNA replication origin firing

Scientific Reports, 2021
Faithful genome duplication requires regulation of origin firing to determine loci, timing and efficiency of replisome generation. Established kinase targets for eukaryotic origin firing regulation are the Mcm2-7 helicase, Sld3/Treslin/TICRR and Sld2 ...
Pedro Ferreira, Verena Höfer, Nora Kronshage, Anika Marko, Karl-Uwe Reusswig, Bilal Tetik, Christoph Dießel, Kerstin Köhler, Nikolai Tschernoster, Janine Altmüller, Nina Schulze, Boris Pfander, Dominik Boos   +12 more
doaj   +1 more source

The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer

Nature Communications, 2022
Here the authors describe multiple structures of the replicative helicase MCM2-7 in complex with Dbf4-dependent kinase (DDK). These structures reveal why the kinase specifically recognizes the MCM2-7 double-hexamer over the single-hexamer and explain how
Almutasem Saleh, Yasunori Noguchi, Ricardo Aramayo, Marina E. Ivanova, Kathryn M. Stevens, Alex Montoya, S. Sunidhi, Nicolas Lopez Carranza, Marcin J. Skwark, Christian Speck   +9 more
doaj   +1 more source

The Contribution of Lysosomes to DNA Replication

Cells, 2021
Lysosomes, acidic, membrane-bound organelles, are not only the core of the cellular recycling machinery, but they also serve as signaling hubs regulating various metabolic pathways. Lysosomes maintain energy homeostasis and provide pivotal substrates for
Joanna Maria Merchut-Maya, Apolinar Maya-Mendoza   +1 more
doaj   +1 more source

Cryo-EM structures reveal that RFC recognizes both the 3′- and 5′-DNA ends to load PCNA onto gaps for DNA repair

eLife, 2022
RFC uses ATP to assemble PCNA onto primed sites for replicative DNA polymerases δ and ε. The RFC pentamer forms a central chamber that binds 3′ ss/ds DNA junctions to load PCNA onto DNA during replication.
Fengwei Zheng, Roxana Georgescu, Nina Y Yao, Huilin Li, Michael E O'Donnell   +4 more
doaj   +1 more source

Unscheduled DNA replication in G1 causes genome instability and damage signatures indicative of replication collisions

Nature Communications, 2022
Reusswig et al. use engineered systems to force DNA replication in the G1 phase of the cell cycle. This unscheduled G1 replication shows hallmarks of S phase replication, but leads to over-replication and DNA breaks from replication collisions.
Karl-Uwe Reusswig, Julia Bittmann, Martina Peritore, Mathilde Courtes, Benjamin Pardo, Michael Wierer, Matthias Mann, Boris Pfander   +7 more
doaj   +1 more source

Depletion of Uhrf1 inhibits chromosomal DNA replication in Xenopus egg extracts [PDF]

, 2013
UHRF1 (ubiquitin-like, containing PHD and RING finger domains 1) has a well-established role in epigenetic regulation through the recognition of various histone marks and interaction with chromatin-modifying proteins.
Taylor, Elaine, Price, Jordan, Bonsu-Dartnall, Nicola, Lindsay, Howard   +3 more
core   +4 more sources