Detection of elsamicin‐DNA binding specificity by restriction enzyme cleavage [PDF]
, 1992 A. Parraga, José Portugal
openalex +1 more source
Establishment and biological characterization of radioresistant colorectal cancer cell lines
FEBS Open Bio, EarlyView.Under ionizing radiation exposure, radiation‐sensitive cancer cells exhibit oxidative stress and DNA damage, while radiation‐resistant cancer cells exhibit strong antioxidant properties and DNA damage repair. Radiotherapy resistance is a major cause of recurrence and metastasis in colorectal cancer (CRC).
Tian‐Yin Qu +10 more
wiley +1 more source
UniZyme: A Unified Protein Cleavage Site Predictor Enhanced with Enzyme Active-Site Knowledge [PDF]
arXivEnzyme-catalyzed protein cleavage is essential for many biological functions. Accurate prediction of cleavage sites can facilitate various applications such as drug development, enzyme design, and a deeper understanding of biological mechanisms. However, most existing models are restricted to an individual enzyme, which neglects shared knowledge of ...
arxiv
Isolation and Preliminary Characterization of Mitochondrial DNA From the Thatching Ant, Formica obscuripes [PDF]
, 1995 A simple and relatively quick procedure was developed for the extraction of mitochondrial DNA from the thatching ant Formica obscuripes . DNA obtained using this procedure was analyzed both after digestion with restriction enzymes (Eco R1 and Bam H1) and
Dover, Deborah
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PCR-Restriction Enzyme Analysis for Detection of Candida DNA in Blood from Febrile Patients with Hematological Malignancies [PDF]
, 1999 Giulia Morace +8 more
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FEBS Open Bio, EarlyView.The tumor microenvironment is a dynamic, multifaceted complex system of interdependent cellular, biochemical, and biophysical components. Three‐dimensional in vitro models of the tumor microenvironment enable a better understanding of these interactions and their impact on cancer progression and therapeutic resistance.
Salma T. Rafik +3 more
wiley +1 more source
Nonhomologous End Joining during Restriction Enzyme-Mediated DNA Integration in Saccharomyces cerevisiae [PDF]
, 1998 P. Manivasakam, Robert H. Schiestl
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FEBS Open Bio, EarlyView.SLC7A11 frequently migrates faster in SDS‐PAGE. The present study found that the high hydrophobicity of SLC7A11 causes its anomalous migration in SDS‐PAGE with a low concentration of acrylamide gel. Replacing isoleucine with asparagine reduced hydrophobicity and restored its normal migration at 55 kDa, revealing the role of hydrophobicity and gel ...
Nsengiyumva Emmanuel +13 more
wiley +1 more source
Combining oligo pools and Golden Gate cloning to create protein variant libraries or guide RNA libraries for CRISPR applications [PDF]
arXivOligo pools are array-synthesized, user-defined mixtures of single-stranded oligonucleotides that can be used as a source of synthetic DNA for library cloning. While currently offering the most affordable source of synthetic DNA, oligo pools also come with limitations such as a maximum synthesis length (approximately 350 bases), a higher error rate ...
arxiv