I-PREFR: Inverse PCR-Based Restriction Enzyme FRee Unidirectional Strategy for Rapid Markerless Chromosomal Gene Deletion and Reconstitution in Bacteria Using Suicide Vectors. [PDF]
Bio ProtocRana R, Sharma A, Dutta A, Patil PB.
europepmc +1 more source
Evaluation of a Quantitative DNA Methylation Analysis Technique using Methylation-Sensitive/Dependent Restriction Enzymes and Real-Time PCR [PDF]
, 2006 Christopher C. Oakes +3 more
openalex +1 more source
H2O2‐Generating Advanced Nanomaterials for Cancer Treatment
Advanced Functional Materials, EarlyView.H2O2‐generating nanoplatforms can exploit tumor redox imbalance for O2 and toxic reactive oxygen species generation, leading to hypoxia reversal, and apoptosis of cancer cells, respectively. This review highlights the mechanisms of these nanoplatforms, including exogenous H₂O₂ delivery, endogenous amplification, and metal peroxides, which leads to ...
Kiyan Musaie +8 more
wiley +1 more source
Real-time observation of DNA looping dynamics of Type IIE restriction enzymes NaeI and NarI [PDF]
, 2006 Bram van den Broek +4 more
openalex +1 more source
Advanced Functional Materials, EarlyView.Oct4‐nanoscript, a biomimetic nanoparticle‐based artificial transcription factor, precisely regulates cellular rejuvenation by activating Oct4 target genes, restoring epigenetic marks, and reducing DNA damage. In a progeria model, it effectively rescued aging‐associated pathologies and extended lifespan.
Hongwon Kim +8 more
wiley +1 more source
APOBEC3 Proteins: From Antiviral Immunity to Oncogenic Drivers in HPV-Positive Cancers. [PDF]
VirusesCastilha EP +5 more
europepmc +1 more source
Intraoral Drug Delivery: Bridging the Gap Between Academic Research and Industrial Innovations
Advanced Functional Materials, EarlyView.Intraoral drug delivery offers a promising route for systemic and localized therapies, yet challenges such as enzymatic degradation, limited permeability, and microbial interactions hinder efficacy. This figure highlights innovative strategies—mucoadhesive materials, enzyme inhibitors, and permeation enhancers—to overcome these barriers.
Soheil Haddadzadegan +4 more
wiley +1 more source
Protocol to quantify site-specific carbohydrate sulfation at immune checkpoint proteins in mammalian cells. [PDF]
STAR ProtocChou WC, Chen WT, Jou YS, Shen CY.
europepmc +1 more source
DNA sequencing analysis of several G6PD variants previously defined by PCR-restriction enzyme analysis [PDF]
, 2006 Gerardo Vaca, Eliakym Arámbula‐Meraz
openalex +1 more source
Advanced Functional Materials, EarlyView.A visible light‐responsive polyacrylamide‐azobenzene hydrogel enables safe, reversible stiffness control for studying cell mechanobiology without harmful UV exposure. This approach reveals stem cells respond rapidly to mechanical changes, showing altered shape and protein distribution within one hour.
Aafreen Ansari +11 more
wiley +1 more source