Results 241 to 250 of about 397,302 (354)

Brief guide to gene cloning. [PDF]

Mol Cells
Hong W, Ha SG, Kwon HC, Lee SV.
europepmc   +1 more source

Bioorthogonal Engineering of Cellular Microenvironments Using Isonitrile Ligations

Advanced Functional Materials, EarlyView.
Highly selective chemistries are required for fabrication and post‐cross–linking modification of cell‐encapsulating hydrogels used in tissue engineering applications. Isonitrile ligation reactions represent a promising class of bioorthogonal chemistries for engineering hydrogel‐based cellular microenvironments. Isonitrile‐based hydrogels are stable and
Ping Zhou, Lauren Brown, Christopher M. Madl   +2 more
wiley   +1 more source

PCR-RFLP assays to detect recessive lethal alleles in Landrace and Duroc pigs in Vietnam. [PDF]

J Vet Diagn Invest
Bui APN, Nguyen NM, Le Q.
europepmc   +1 more source

Reliable Detection Of DNA Cytosine Methylation at CpNpG Sites Using the Engineered Restriction Enzyme Eco RII-C

, 2005
Detlev H. Krüger, Monika Reuter
openalex   +1 more source

An investigation of the structural requirements for ATP hydrolysis and DNA cleavage by the EcoKI Type I DNA restriction and modification enzyme [PDF]

, 2011
Gareth A. Roberts, Laurie P. Cooper, John H. White, Tsueu-Ju Su, Jakob T. Zipprich, Paul Geary, C. D. Kennedy, David T. F. Dryden   +7 more
openalex   +1 more source

Photothermal Hydrogel with Mn3O4 Nanoparticles Alleviates Intervertebral Disc Degeneration by Scavenging ROS and Regulating Extracellular Matrix Metabolism

Advanced Functional Materials, EarlyView.
The MPTT‐nanozyme‐hydrogel system (Mn3O4@ChS‐HA) provides a multifunctional therapeutic strategy for intervertebral disc degeneration (IVDD), effectively targeting oxidative stress and enhancing AF repair by restoring extracellular matrix (ECM) and redox homeostasis.
Yangyang Chen, Chan Xue, Feifei Ni, Binwu Hu, Miaoheng Yan, He Zhang, Yuxiang Hu, Xiaoyao Peng, Guangfang Li, Yu Han, Hongjian Liu, Zengwu Shao, Qin Wang, Yulong Wei   +13 more
wiley   +1 more source

PCR-Free Site-Directed Mutagenesis on Repetitive Sequences Using Single-Stranded DNA-Assisted Double-Stranded DNA Nicking by DNAzymes. [PDF]

Angew Chem Int Ed Engl
Lyu M, Kong L, Shao X, Lu Y.
europepmc   +1 more source

Faculty Opinions recommendation of Crystal structure of DNA sequence specificity subunit of a type I restriction-modification enzyme and its functional implications.

, 2005
Malcolm F. White
openalex   +1 more source

Versatile Cell Penetrating Peptide for Multimodal CRISPR Gene Editing in Primary Stem Cells

Advanced Functional Materials, EarlyView.
CRISPR machinery in diverse molecular formats (DNA, RNA, and ribonucleic protein) is complexed into nanoparticles with the cell‐friendly arginine‐alanine‐leucine‐alanine (RALA) cell‐penetrating peptide. Nanoparticles are delivered to primary mesenchymal stem cells ex vivo or locally in vivo to facilitate multimodal CRISPR gene editing. This RALA‐CRISPR
Joshua P. Graham, Jose Gabriel Castro, Lisette C. Werba, Joseph Amitrano, Luke Fardone, Kevin P. Francis, Anand Ramamurthi, Michael J. Layden, Helen O. McCarthy, Tomas Gonzalez‐Fernandez   +9 more
wiley   +1 more source

UniClo: scarless hierarchical DNA assembly without sequence constraint. [PDF]

Nucleic Acids Res
Flores-Fernández CN, Lin D, Robins K, O'Callaghan CA.   +3 more
europepmc   +1 more source