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Programmable DNA-Guided Artificial Restriction Enzymes.
ACS Synthetic Biology, 2017Restriction enzymes are essential tools for recombinant DNA technology that have revolutionized modern biological research. However, they have limited sequence specificity and availability. Here we report a Pyrococcus furiosus Argonaute (PfAgo) based platform for generating artificial restriction enzymes (AREs) capable of recognizing and cleaving DNA ...
Behnam Enghiad, Huimin Zhao
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What is the function of DNA restriction enzymes
Trends in Biochemical Sciences, 1977The message of these figures is that in terms purely of economic cost it would make sense to mount a global vaccina- tion program against smallpox, even if the disease could never be eliminated. The hope of eradicating smallpox altogether rests on the fact that there seems to be no animal reservoir from which the human population can be reinfected, nor
W. Arber
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Structures and mechanisms of DNA restriction and modification enzymes
Quarterly Reviews of Biophysics, 1979DNA restriction and modification enzymes are responsible for the hostspecific barriers to interstrain and interspecies transfer of genetic information that have been observed in a variety of bacterial cell types. Although the phenomenon of host specificity was initially observed in the early 1950s (Luria & Human, 1952; Bertani & Weigle, 1953 ...
P. Modrich
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DNA restriction enzymes and RFLPs in medicine.
Methods of biochemical analysis, 1992J. Kant
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On the structure and operation of type I DNA restriction enzymes.
Journal of Molecular Biology, 1999Type I DNA restriction enzymes are large, molecular machines possessing DNA methyltransferase, ATPase, DNA translocase and endonuclease activities. The ATPase, DNA translocase and endonuclease activities are specified by the restriction (R) subunit of the enzyme.
Graham P Davies +5 more
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Inhibition of DNA restriction enzyme digestion by anthracyclines
La Ricerca in Clinica e in Laboratorio, 1988The inhibition of restriction enzyme digestion of lambda phage DNA by anthracyclines (i.e., adriamycin, daunomycin, epirubicin, idarubicin and esorubicin) commonly used in the treatment of human leukemia and cancer has been studied in vitro. The anthracyclines used inhibit DNA digestion by SmaI, AvaII, HaeIII, HhaI and HpaII, which cut DNA at guanine ...
G. Corneo +3 more
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Restriction enzyme analysis of the Plasmid ColIb DNA
Molecular and General Genetics MGG, 1979Plasmid ColIb (61.5 Mdal) was digested with restriction enzymes EcoRI and HindIII. The DNA digestion products were separated by electrophoresis on 1.2% agarose gels. There were identified 22 fragments of ColIb DNA generated by the endonuclease EcoRI and 21 fragments produced by HindIII. Molecular weights of the fragments were estimated.
A, Skorupska +2 more
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Restriction enzyme cleavage of ultraviolet-damaged DNA
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1982SV40 and pBR322 DNAs damaged by ultraviolet light were cleaved abnormally by several restriction enzymes because of damage to pyrimidines in the recognition sequences. The use of a tandemly duplicated plasmid provided a particularly sensitive target molecule for detecting pyrimidine dimers and other possible photoproducts.
J E, Cleaver, L, Samson, G H, Thomas
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1987
The discovery of restriction and modification enzymes, which proved to be a major turning point in the progress of molecular biology, was a consequence of a bacteriological observation in the early 1950s (Luria and Human, 1952; Bertani and Weigle, 1953).
Alan D. B. Malcolm, Georges Snounou
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The discovery of restriction and modification enzymes, which proved to be a major turning point in the progress of molecular biology, was a consequence of a bacteriological observation in the early 1950s (Luria and Human, 1952; Bertani and Weigle, 1953).
Alan D. B. Malcolm, Georges Snounou
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DNA Restriction Enzyme from E. coli
Nature, 1968An endonuclease which degrades foreign DNA has been isolated. The enzyme requires S-adenosylmethionine, ATP and Mg++.
M, Meselson, R, Yuan
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