Results 51 to 60 of about 673,449 (404)

Using of Data Base to Determine the Restriction Sites and Drawing Restriction Map for Lipase Gene from Bacillus stearothermophilus

open access: yesمجلة مركز بحوث التقنيات الاحيائية, 2011
pecific data base was used for restriction enzymes (rebase) and related proteins, to design executive program in quick basic language to determine the restriction sites and drawing restriction map for the complete sequence of lipase gene from Bacillus ...
Hameed M. Jasim
doaj   +1 more source

Microarray-Based Analysis of Methylation Status of CpGs in Placental DNA and Maternal Blood DNA--Potential New Epigenetic Biomarkers for Cell Free Fetal DNA-Based Diagnosis. [PDF]

open access: yesPLoS ONE, 2015
Epigenetic markers for cell free fetal DNA in the maternal blood circulation are highly interesting in the field of non-invasive prenatal testing since such markers will offer a possibility to quantify the amount of fetal DNA derived from different ...
Lotte Hatt   +7 more
doaj   +1 more source

Isolation and Physical Mapping of the Mitochondrial Chromosome From the Zygomycete Mucor Racemosus. [PDF]

open access: yes, 1990
Mitochondria were isolated from Mucor racemosus and DNA was purified from these organelles by cesium chloride/ethidium bromide isopycnic centrifugation. Examination of mitochondrial DNA samples with a transmission electron microscope revealed a circular ...
Schramke, Mary Louise
core   +2 more sources

An Efficient PCR-RFLP Method for the Rapid Identification of Korean Pyropia Species

open access: yesMolecules, 2017
The present study utilizes polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using partial plastid rbcL and mitochondrial trnC–trnP gene sequences to distinguish the six representative Pyropia species produced via ...
Yonguk Kim, Sung-Je Choi, Chulyung Choi
doaj   +1 more source

EcoRV catalysis with a pre-bent substrate

open access: yesAIP Advances, 2015
Enzymes are deformable molecules which often adapt their conformation to the substrate’s geometry. In the case of restriction enzymes acting on DNA, the substrate (DNA) is deformable also.
Daniel S. Sanchez, Giovanni Zocchi
doaj   +1 more source

REHUNT: a reliable and open source package for restriction enzyme hunting

open access: yesBMC Bioinformatics, 2018
Background Restriction enzymes are used frequently in biotechnology. However, manual mining of restriction enzymes is challenging. Furthermore, integrating available restriction enzymes into different bioinformatics systems is necessary for many ...
Yu-Huei Cheng   +2 more
doaj   +1 more source

Genome-wide DNA methylation profiling using the methylation-dependent restriction enzyme LpnPI

open access: yesGenome Research, 2018
DNA methylation is a well-known epigenetic modification that plays a crucial role in gene regulation, but genome-wide analysis of DNA methylation remains technically challenging and costly.
R. Boers   +8 more
semanticscholar   +1 more source

An efficient method for DNA extraction from Cladosporioid fungi [PDF]

open access: yes, 2010
We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A.   +3 more
core   +2 more sources

APOBECs and Herpesviruses

open access: yesViruses, 2021
The apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (APOBEC) family of DNA cytosine deaminases provides a broad and overlapping defense against viral infections.
Adam Z. Cheng   +8 more
doaj   +1 more source

Breaking RAD: an evaluation of the utility of restriction site‐associated DNA sequencing for genome scans of adaptation

open access: yesMolecular Ecology Resources, 2016
Understanding how and why populations evolve is of fundamental importance to molecular ecology. Restriction site‐associated DNA sequencing (RADseq), a popular reduced representation method, has ushered in a new era of genome‐scale research for assessing ...
D. Lowry   +6 more
semanticscholar   +1 more source

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