Results 31 to 40 of about 2,453,331 (300)

Enhancement of PCRs by Partial Restriction Digestion of Genomic Templates

BioTechniques, 1999
Genomic DNA preparations derived from mammalian cells can often exhibit poor template activity in PCR, particularly when carried out on target sequences present at low copy number. Using genomic DNA bearing SV40 sequences integrated into host chromosomal
Raymond Vázquez, Mark L. Steinberg
doaj   +1 more source

Burkholderia pseudomallei sequencing identifies genomic clades with distinct recombination, accessory, and epigenetic profiles. [PDF]

, 2014
Burkholderia pseudomallei (Bp) is the causative agent of the infectious disease melioidosis. To investigate population diversity, recombination, and horizontal gene transfer in closely related Bp isolates, we performed whole-genome sequencing (WGS) on ...
Studholme, David J., Holden, Mathew TG, Ong, C, Beacham, I, Parkhill, J, Harting, J, Chen, Swaine L., Mehershahi, K, Holden, Matthew T. G., Nandi, T, T.G. Holden, Matthew, Didelot, X, Peak, I, Baybayan, Primo, Peak, Ian, Sim, B, Hoon, CH, Nandi, Tannistha, Boddey, J, Boddey, Justin, Boddey, Justin A., Holden, Matthew, J. Studholme, David, Baybayan, P, Michell, S, Essex-Lopresti, AE, Guo, Yan, Sim, Bernice, Didelot, Xavier, Titball, Richard W., Chen, SL, Smither, Sophie, Smiters, Sophie, Beacham, Ifor, Holden, Matthew TG, Smither, S, How, LC, Nelson, Michelle, Ong, Catherine, Tan, P, Wang, S, Aw, LT, Nelson, M, Mitchell, Stephen, Michell, Stephen, Aw, Lay Tin, E. Essex-Lopresti, Angela, Chen, Swaine, Chen, Swaine L, Titball, Richard, Mehrashahi, Kurosh, Hoon, Chua Hui, Boddey, Justin A, Sarkar-Tyson, M, Parkhill, Julian, Mehershahi, Kurosh, Guo, Y, Tan, Patrick, How, Lee Chee, W. Titball, Richard, Sarkar-Tyson, Mitali, Wang, Susana, Essex-Lopresti, Angela, Studholme, David J, Harting, John   +64 more
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Resolution of Joint Molecules by RuvABC and RecG Following Cleavage of the Escherichia coli Chromosome by EcoKI [PDF]

, 2009
DNA double-strand breaks can be repaired by homologous recombination involving the formation and resolution of Holliday junctions. In Escherichia coli, the RuvABC resolvasome and the RecG branch-migration enzyme have been proposed to act in alternative ...
Okely Ewa, Leach David, David Leach, Wardrope, Laura, Leach, David, Ewa Okely, Laura Wardrope, Okely, Ewa, Wardrope Laura   +8 more
core   +2 more sources

DNA methylation assessed by SMRT sequencing is linked to mutations in Neisseria meningitidis isolates [PDF]

, 2015
The Gram-negative bacterium Neisseria meningitidis features extensive genetic variability. To present, proposed virulence genotypes are also detected in isolates from asymptomatic carriers, indicating more complex mechanisms underlying variable ...
Gerd Pluschke, Christoph D. Schmid (72046), Schmid, Christoph D., Mane, Shrikant, Korlach, Jonas, Tyson A. Clark (105783), Katharina Röltgen, Wang, Guilin, Araceli Lamelas, Jonas Korlach (105788), Shrikant Mane, Araceli Lamelas (128475), Christoph D Schmid, Mohamad R Abdul Sater, Clark, Tyson A., Röltgen, Katharina, Gerd Pluschke (7855), Katharina Röltgen (120045), Guilin Wang, Sater, Mohamad R. Abdul, Tyson A Clark, Mohamad R. Abdul Sater (836669), Jonas Korlach, Guilin Wang (37620), Shrikant Mane (106631), Lamelas, Araceli, Pluschke, Gerd   +26 more
core   +1 more source

Straightforward Procedure for Laboratory Production of DNA Ladder

Journal of Nucleic Acids, 2012
DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare a DNA ladder that consists of 10 fragments from 100 to 1000 bp.
Vo Thi Thuong Lan, Pham Thi Thanh Loan, Pham Anh Thuy Duong, Le Thi Thanh, Ngo Thi Ha, Ta Bich Thuan   +5 more
doaj   +1 more source

Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities. [PDF]

PLoS Biology, 2016
The creation of restriction enzymes with programmable DNA-binding and -cleavage specificities has long been a goal of modern biology. The recently discovered Type IIL MmeI family of restriction-and-modification (RM) enzymes that possess a shared target ...
Scott J Callahan, Yvette A Luyten, Yogesh K Gupta, Geoffrey G Wilson, Richard J Roberts, Richard D Morgan, Aneel K Aggarwal   +6 more
doaj   +1 more source

Molecular cloning and physical mapping of murine cytomegalovirus DNA [PDF]

, 1983
Murine cytomegalovirus (MCMV) Smith strain DNA is cleaved by restriction endonuclease HindIII into 16 fragments, ranging in size from 0.64 to 22.25 megadaltons.
Ebeling, Angelika, Koszinowski, Ulrich H., Knust, Elisabeth, Keil, Günther M.   +3 more
core   +1 more source

Suppression of APOBEC3-mediated restriction of HIV-1 by Vif

Frontiers in Microbiology, 2014
The APOBEC3 restriction factors are a family of deoxycytidine deaminases that are able to suppress replication of viruses with a single-stranded DNA intermediate by inducing mutagenesis and functional inactivation of the virus.
Yuqing eFeng, Tayyba T Baig, Robin P Love, Linda eChelico   +3 more
doaj   +1 more source

The Hyperthermophilic Restriction-Modification Systems of Thermococcus kodakarensis Protect Genome Integrity

Frontiers in Microbiology, 2021
Thermococcus kodakarensis (T. kodakarensis), a hyperthermophilic, genetically accessible model archaeon, encodes two putative restriction modification (R-M) defense systems, TkoI and TkoII.
Kelly M. Zatopek, Brett W. Burkhart, Richard D. Morgan, Alexandra M. Gehring, Kristin A. Scott, Thomas J. Santangelo, Andrew F. Gardner   +6 more
doaj   +1 more source

Time-resolved fluorescence studies of nucleotide flipping by restriction enzymes [PDF]

, 2009
Restriction enzymes Ecl18kI, PspGI and EcoRII-C, specific for interrupted 5-bp target sequences, flip the central base pair of these sequences into their protein pockets to facilitate sequence recognition and adjust the DNA cleavage pattern. We have used
Neely, Robert K., Jones, Anita C., Tamulaitis, Gintautas, Kubala, Marta, Neely, Robert K; id_orcid, Chen, Kai, Siksnys, Virginijus, Jones, Anita C   +7 more
core   +1 more source