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Structures of the type I DNA restriction enzymes [PDF]

Proceedings of the National Academy of Sciences, 2017
The article by Liu et al. (1) on the structure of type I DNA restriction and modification enzymes purports to significantly advance our understanding of these enzymes and proposes a model for their operation. While the partial structure of one of these enzymes is interesting and defines the interface between some of the subunits, the article contains ...
Dryden DTF.
openaire   +3 more sources

On the DNA cleavage mechanism of Type I restriction enzymes [PDF]

Nucleic Acids Research, 2005
Although the DNA cleavage mechanism of Type I restriction-modification enzymes has been extensively studied, the mode of cleavage remains elusive. In this work, DNA ends produced by EcoKI, EcoAI and EcoR124I, members of the Type IA, IB and IC families, respectively, have been characterized by cloning and sequencing restriction products from the ...
Jindrova E, Schmid-Nuoffer S, Hamburger F, Janscak P, Bickle TA   +4 more
openaire   +6 more sources

Structure and operation of the DNA-translocating type I DNA restriction enzymes [PDF]

Genes & Development, 2012
Type I DNA restriction/modification (RM) enzymes are molecular machines found in the majority of bacterial species. Their early discovery paved the way for the development of genetic engineering. They control (restrict) the influx of foreign DNA via horizontal gene transfer into the bacterium while maintaining sequence-specific methylation ...
Kennaway, Christopher K., Taylor, James E., Song, Chun Feng, Potrzebowski, Wojciech, Nicholson, William, White, John H., Swiderska, Anna, Obarska-Kosinska, Agnieszka, Callow, Philip, Cooper, Laurie P., Roberts, Gareth A., Artero, Jean-Baptiste, Bujnicki, Janusz M., Trinick, John, Kneale, G. Geoff, Dryden, David T.F.   +15 more
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Type II Restriction of 2-Aminoadenosine (dZ)-Modified DNA and Production of dZ-Modified Plasmid in E. coli [PDF]

Viruses
The modified DNA base 2,6 aminopurine (2-aminoadenine, (d)Z base) was originally found in phages to counteract host-encoded restriction systems. However, only a limited number of restriction endonucleases (REases) have been tested on dZ-modified DNA ...
Weiwei Yang, Michael S. Kuska, Nan Dai, Laurence M. Ettwiller, Ivan R. Corrêa, Shuang-Yong Xu   +5 more
doaj   +2 more sources

Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering

Fermentation, 2023
Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
doaj   +1 more source

REBASE--restriction enzymes and DNA methyltransferases [PDF]

Nucleic Acids Research, 2004
REBASE is a comprehensive database of information about restriction enzymes, DNA methyltransferases and related proteins involved in restriction-modification. It contains both published and unpublished work with information about recognition and cleavage sites, isoschizomers, commercial availability, crystal and sequence data.
Richard J. Roberts, Tamas Vincze, Janos Posfai, Dana Macelis   +3 more
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Restriction enzyme digestion of hemimethylated DNA [PDF]

Nucleic Acids Research, 1981
Hemimethylated duplex DNA of the bacteriophage phi X 174 was synthesized using primed repair synthesis is in vitro with E. coli DNA polymerase I followed by ligation to produce the covalently closed circular duplex (RFI). Single-stranded phi X DNA was used as a template, a synthetic oligonucleotide as primer and 5-methyldeoxycytidine-5'-triphosphate ...
Y, Gruenbaum, H, Cedar, A, Razin
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Revised selection criteria for candidate restriction enzymes in genome walking. [PDF]

PLoS ONE, 2012
A new method to improve the efficiency of flanking sequence identification by genome walking was developed based on an expanded, sequential list of criteria for selecting candidate enzymes, plus several other optimization steps.
Ali Taheri, Stephen J Robinson, Isobel Parkin, Margaret Y Gruber   +3 more
doaj   +1 more source

In vitro Type II Restriction of Bacteriophage DNA With Modified Pyrimidines

Frontiers in Microbiology, 2020
To counteract host-encoded restriction systems, bacteriophages (phages) incorporate modified bases in their genomes. For example, phages carry in their genomes modified pyrimidines such as 5-hydroxymethyl-cytosine (5hmC) in T4gt deficient in α- and β ...
Kiersten Flodman, Ivan R. Corrêa, Nan Dai, Peter Weigele, Shuang-yong Xu   +4 more
doaj   +1 more source

DNA polymorphism investigation among local Brucella isolates [PDF]

Iraqi Journal of Veterinary Sciences, 2009
The present study was undertaken to investigate the deoxyribonucleic acid (DNA) polymorphism finger printing among DNA of local Brucella isolates which is a new method in molecular epidemiological studies.
S. A. AL-Bayatti ¹ and A. N. Al-Thwani ²
doaj   +1 more source