Results 21 to 30 of about 2,453,331 (300)

Extensive DNA mimicry by the ArdA anti-restriction protein and its role in the spread of antibiotic resistance [PDF]

, 2009
The ardA gene, found in many prokaryotes including important pathogenic species, allows associated mobile genetic elements to evade the ubiquitous Type I DNA restriction systems and thereby assist the spread of resistance genes in bacterial populations ...
Blakely, Garry W., Dryden, David T. F., White, John H., McMahon, Stephen A., Cooper, Laurie P., Naismith, James H., Roberts, Gareth A., Liu, Huanting, Oke, Muse, Naismith, James Henderson, Carter, Lester G., Sanghvi, Bansi, Walkinshaw, Malcolm D., Johnson, Kenneth A.   +13 more
core   +1 more source

A physical map of human Alu repeats cleavage by restriction endonucleases

BMC Genomics, 2008
Background Alu repetitive elements are the abundant sequences in human genome. Diversity of DNA sequences of these elements makes difficult the construction of theoretical patterns of Alu repeats cleavage by restriction endonucleases.
Chernukhin Valery A, Tomilov Victor N, Abdurashitov Murat A, Degtyarev Sergey   +3 more
doaj   +1 more source

Impact of target site distribution for Type I restriction enzymes on the evolution of methicillin-resistant Staphylococcus aureus (MRSA) populations. [PDF]

, 2013
A limited number of Methicillin-resistant Staphylococcus aureus (MRSA) clones are responsible for MRSA infections worldwide, and those of different lineages carry unique Type I restriction-modification (RM) variants.
Dryden, David T. F., White, John H., Stephanou, AS, Cooper, Laurie P., Cooper, LP, Houston, PJ, Lindsay, JA, Chen, K, Dryden, DT, Roberts, Gareth A., Lindsay, Jodi A., Chen, Kai, Roberts, GA, White, JH, Stephanou, Augoustinos S., Houston, Patrick J.   +15 more
core   +1 more source

Fused eco29kIR- and M genes coding for a fully functional hybrid polypeptide as a model of molecular evolution of restriction-modification systems

BMC Evolutionary Biology, 2011
Background The discovery of restriction endonucleases and modification DNA methyltransferases, key instruments of genetic engineering, opened a new era of molecular biology through development of the recombinant DNA technology.
Solonin Alexander S, Mokrishcheva Marina L, Nikitin Dmitri V   +2 more
doaj   +1 more source

Universal Linker and Ligation Procedures for Construction of Genomic DNA Libraries Enriched for Microsatellites

BioTechniques, 1999
Microsatellite loci are highly informative genetic markers useful for population genetic studies, linkage mapping and parentage determination. Methods to identify novel microsatellite loci commonly use subtractive hybridization to enrich smallinsert ...
Matthew B. Hamilton, Elaine L. Pincus, Anthony Di Fiore, Robert C. Fleischer   +3 more
doaj   +1 more source

Biomolecular computers with multiple restriction enzymes

Genetics and Molecular Biology, 2017
The development of conventional, silicon-based computers has several limitations, including some related to the Heisenberg uncertainty principle and the von Neumann “bottleneck”.
Sebastian Sakowski, Tadeusz Krasinski, Jacek Waldmajer, Joanna Sarnik, Janusz Blasiak, Tomasz Poplawski   +5 more
doaj   +1 more source

Fingerprinting of Bacterial Genomes by Amplification of DNA Fragments Surrounding Rare Restriction Sites

BioTechniques, 2001
A method for generating limited representations of total bacterial DNA, without prior knowledge of the DNA sequence, has been developed. This method consists of three steps: digestion with two restriction enzymes, ligation of two oligonucleotide adapters
Aleksander Masny, Andrzej Plucienniczak
doaj   +1 more source

Restriction enzyme mapping of vaccinia virus DNA [PDF]

Journal of Virology, 1982
The cleavage sites for the restriction enzymes Bg/I, HindIII, KpnI, SalI, SmaI, and XhoI were located, from primary data, on the DNA isolated from the WR strain of vaccinia virus. Bg/I and SmaI divide the DNA into five segments which can be isolated by sucrose gradient centrifugation.
openaire   +2 more sources

Highlights of the DNA cutters: a short history of the restriction enzymes [PDF]

Nucleic Acids Research, 2013
AbstractIn the early 1950’s, ‘host-controlled variation in bacterial viruses’ was reported as a non-hereditary phenomenon: one cycle of viral growth on certain bacterial hosts affected the ability of progeny virus to grow on other hosts by either restricting or enlarging their host range.
Loenen, Wil A M, Dryden, David T F, Raleigh, Elisabeth A, Wilson, Geoffrey G, Murray, Noreen E   +4 more
openaire   +3 more sources

Structural and mutagenic analysis of the RM controller protein C.Esp1396I [PDF]

, 2014
Bacterial restriction-modification (RM) systems are comprised of two complementary enzymatic activities that prevent the establishment of foreign DNA in a bacterial cell: DNA methylation and DNA restriction.
Martin, Richard N. A., Kneale, Geoff, John E McGeehan, Geoff Kneale, Geoff Kneale (22086), Mcgeehan, John, Richard N A Martin, Richard N. A. Martin (571238), John E. McGeehan (431702)   +8 more
core   +2 more sources