Results 21 to 30 of about 227,260 (265)

A physical map of human Alu repeats cleavage by restriction endonucleases

BMC Genomics, 2008
Background Alu repetitive elements are the abundant sequences in human genome. Diversity of DNA sequences of these elements makes difficult the construction of theoretical patterns of Alu repeats cleavage by restriction endonucleases.
Chernukhin Valery A, Tomilov Victor N, Abdurashitov Murat A, Degtyarev Sergey   +3 more
doaj   +1 more source

Fused eco29kIR- and M genes coding for a fully functional hybrid polypeptide as a model of molecular evolution of restriction-modification systems

BMC Evolutionary Biology, 2011
Background The discovery of restriction endonucleases and modification DNA methyltransferases, key instruments of genetic engineering, opened a new era of molecular biology through development of the recombinant DNA technology.
Solonin Alexander S, Mokrishcheva Marina L, Nikitin Dmitri V   +2 more
doaj   +1 more source

Universal Linker and Ligation Procedures for Construction of Genomic DNA Libraries Enriched for Microsatellites

BioTechniques, 1999
Microsatellite loci are highly informative genetic markers useful for population genetic studies, linkage mapping and parentage determination. Methods to identify novel microsatellite loci commonly use subtractive hybridization to enrich smallinsert ...
Matthew B. Hamilton, Elaine L. Pincus, Anthony Di Fiore, Robert C. Fleischer   +3 more
doaj   +1 more source

Biomolecular computers with multiple restriction enzymes

Genetics and Molecular Biology, 2017
The development of conventional, silicon-based computers has several limitations, including some related to the Heisenberg uncertainty principle and the von Neumann “bottleneck”.
Sebastian Sakowski, Tadeusz Krasinski, Jacek Waldmajer, Joanna Sarnik, Janusz Blasiak, Tomasz Poplawski   +5 more
doaj   +1 more source

Fingerprinting of Bacterial Genomes by Amplification of DNA Fragments Surrounding Rare Restriction Sites

BioTechniques, 2001
A method for generating limited representations of total bacterial DNA, without prior knowledge of the DNA sequence, has been developed. This method consists of three steps: digestion with two restriction enzymes, ligation of two oligonucleotide adapters
Aleksander Masny, Andrzej Plucienniczak
doaj   +1 more source

Restriction enzyme mapping of vaccinia virus DNA [PDF]

Journal of Virology, 1982
The cleavage sites for the restriction enzymes Bg/I, HindIII, KpnI, SalI, SmaI, and XhoI were located, from primary data, on the DNA isolated from the WR strain of vaccinia virus. Bg/I and SmaI divide the DNA into five segments which can be isolated by sucrose gradient centrifugation.
openaire   +2 more sources

Enhancement of PCRs by Partial Restriction Digestion of Genomic Templates

BioTechniques, 1999
Genomic DNA preparations derived from mammalian cells can often exhibit poor template activity in PCR, particularly when carried out on target sequences present at low copy number. Using genomic DNA bearing SV40 sequences integrated into host chromosomal
Raymond Vázquez, Mark L. Steinberg
doaj   +1 more source

Straightforward Procedure for Laboratory Production of DNA Ladder

Journal of Nucleic Acids, 2012
DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare a DNA ladder that consists of 10 fragments from 100 to 1000 bp.
Vo Thi Thuong Lan, Pham Thi Thanh Loan, Pham Anh Thuy Duong, Le Thi Thanh, Ngo Thi Ha, Ta Bich Thuan   +5 more
doaj   +1 more source

Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities. [PDF]

PLoS Biology, 2016
The creation of restriction enzymes with programmable DNA-binding and -cleavage specificities has long been a goal of modern biology. The recently discovered Type IIL MmeI family of restriction-and-modification (RM) enzymes that possess a shared target ...
Scott J Callahan, Yvette A Luyten, Yogesh K Gupta, Geoffrey G Wilson, Richard J Roberts, Richard D Morgan, Aneel K Aggarwal   +6 more
doaj   +1 more source

Suppression of APOBEC3-mediated restriction of HIV-1 by Vif

Frontiers in Microbiology, 2014
The APOBEC3 restriction factors are a family of deoxycytidine deaminases that are able to suppress replication of viruses with a single-stranded DNA intermediate by inducing mutagenesis and functional inactivation of the virus.
Yuqing eFeng, Tayyba T Baig, Robin P Love, Linda eChelico   +3 more
doaj   +1 more source