The average spacing of restriction enzyme recognition sites in DNA [PDF]
The discovery of naturally occurring enzymes which cleave DNA at sites specific to particular nucleotide sequences has had a great impact on molecular biology. The function of these enzymes in uivo is to protect bacterial cells from viral invasion by degradation of foreign DNA.
Moore, Gordon P., Moore, Arnold R.
openaire +3 more sources
An Efficient PCR-RFLP Method for the Rapid Identification of Korean Pyropia Species
The present study utilizes polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis using partial plastid rbcL and mitochondrial trnC–trnP gene sequences to distinguish the six representative Pyropia species produced via ...
Yonguk Kim, Sung-Je Choi, Chulyung Choi
doaj +1 more source
Microarray-Based Analysis of Methylation Status of CpGs in Placental DNA and Maternal Blood DNA--Potential New Epigenetic Biomarkers for Cell Free Fetal DNA-Based Diagnosis. [PDF]
Epigenetic markers for cell free fetal DNA in the maternal blood circulation are highly interesting in the field of non-invasive prenatal testing since such markers will offer a possibility to quantify the amount of fetal DNA derived from different ...
Lotte Hatt +7 more
doaj +1 more source
Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA [PDF]
Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested.
Abee, T. +5 more
core +1 more source
EcoRV catalysis with a pre-bent substrate
Enzymes are deformable molecules which often adapt their conformation to the substrate’s geometry. In the case of restriction enzymes acting on DNA, the substrate (DNA) is deformable also.
Daniel S. Sanchez, Giovanni Zocchi
doaj +1 more source
pecific data base was used for restriction enzymes (rebase) and related proteins, to design executive program in quick basic language to determine the restriction sites and drawing restriction map for the complete sequence of lipase gene from Bacillus ...
Hameed M. Jasim
doaj +1 more source
REHUNT: a reliable and open source package for restriction enzyme hunting
Background Restriction enzymes are used frequently in biotechnology. However, manual mining of restriction enzymes is challenging. Furthermore, integrating available restriction enzymes into different bioinformatics systems is necessary for many ...
Yu-Huei Cheng +2 more
doaj +1 more source
A comparison of DNA cleavage by the restriction enzymes SalPI and PstI [PDF]
Methods for obtaining highly active, exonuclease-free, stable preparations of the Streptomyces albus P restriction enzyme SalPI are described. SalPI and its isoschizomer PstI (from the taxonomically distant Providencia stuartii 164) both cleave their recognition sequence (5'-CTGCAG-3') to generate fragments terminating in tetranucleotide 3' extensions ...
J A, Carter +3 more
openaire +2 more sources
Architecturally diverse proteins converge on an analogous mechanism to inactivate Uracil-DNA glycosylase [PDF]
Uracil-DNA glycosylase (UDG) compromises the replication strategies of diverse viruses from unrelated lineages. Virally encoded proteins therefore exist to limit, inhibit or target UDG activity for proteolysis.
Cole, Ambrose R. +11 more
core +1 more source
Type I restriction-modification (RM) systems are comprised of two multi-subunit enzymes, the methyltransferase (∼160 kDa), responsible for methylation of DNA, and the restriction endonuclease (∼400 kDa), responsible for DNA cleavage. Both enzymes share a
James E N Taylor +4 more
doaj +1 more source

