Results 41 to 50 of about 227,260 (265)

Whole genome phylogeny for 21 Drosophila species using predicted 2b-RAD fragments [PDF]

open access: yesPeerJ, 2013
Type IIB restriction endonucleases are site-specific endonucleases that cut both strands of double-stranded DNA upstream and downstream of their recognition sequences.
Arun S. Seetharam, Gary W. Stuart
doaj   +2 more sources

Mapping the evolution of mitochondrial complex I through structural variation

open access: yesFEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin   +2 more
wiley   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

open access: yesFEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

An alternative approach to study the enzymatic specificities of the CfrBI restriction–modification system

open access: yesHeliyon, 2019
Restriction–modification systems (RMS) are the main gene-engineering tools and a suitable model to study the molecular mechanisms of catalysis and DNA–protein interactions.
Marina V. Zakharova   +3 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

open access: yesFEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe   +3 more
wiley   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

open access: yesFEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva   +5 more
wiley   +1 more source

Studi tentang polimorfisme ulat sutera F1 hibrid hasil persilangan ras Jepang dan ras Cina yang berasal dari pusat pembibitan Soppeng dan Temanggung dengan menggunakan enzim restriksi Pst1 dan EcoR1

open access: yesJurnal Ilmu-Ilmu Peternakan, 2015
There were diffferent performance between silkworms breeded in Soppeng and Temanggung. There might be different proportion of parenstock and environment which causing polymorphism. This experiment was to know the genetic band pattern as the effect of the
Nur Cholis
doaj   +1 more source

Restriction Enzyme Analysis of Tomato Chloroplast and Chromoplast DNA [PDF]

open access: yesPlant Physiology, 1986
Plastid DNA was isolated from the chloroplasts of tomato (Lycopersicon esculentum var Traveler 76) leaves and the chromoplasts of ripe tomato fruit. Comparisons of the two DNAs were made by restriction endonuclease analysis using PvuII, HpaI, and Bg1I.
C M, Hunt, R C, Hardison, C D, Boyer
openaire   +2 more sources

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

open access: yesFEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li   +2 more
wiley   +1 more source

Consecutive Cycles of Precise, Unidirectional 14-bp Deletions Using a BseRI/BsgI Trimming Plasmid

open access: yesBioTechniques, 1996
A straightforward method for generating precise, consecutive, unidirectional 14-bp deletions into cloned DNA, adopted from the trimming principle developed by Szybalski and his colleagues, is presented. The method utilizes pTRIM14, a plasm id constructed
Eric A. Ariazi, Michael N. Gould
doaj   +1 more source

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