Results 51 to 60 of about 2,453,331 (300)

APOBECs and Herpesviruses

open access: yesViruses, 2021
The apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (APOBEC) family of DNA cytosine deaminases provides a broad and overlapping defense against viral infections.
Adam Z. Cheng   +8 more
doaj   +1 more source

Restriction Enzyme Analysis of Tomato Chloroplast and Chromoplast DNA [PDF]

open access: yesPlant Physiology, 1986
Plastid DNA was isolated from the chloroplasts of tomato (Lycopersicon esculentum var Traveler 76) leaves and the chromoplasts of ripe tomato fruit. Comparisons of the two DNAs were made by restriction endonuclease analysis using PvuII, HpaI, and Bg1I.
C M, Hunt, R C, Hardison, C D, Boyer
openaire   +2 more sources

PCR-RFLP of mitochondrial DNA of swamp buffaloes in breeding station

open access: yesItalian Journal of Animal Science, 2010
Methods have been devised for detecting polymorphisms in the D-loop regions using the PCR followed by restriction enzyme digestion to reveal restriction fragment length polymorphism.
M. Kamonpatana   +6 more
doaj   +1 more source

Whole genome phylogeny for 21 Drosophila species using predicted 2b-RAD fragments [PDF]

open access: yesPeerJ, 2013
Type IIB restriction endonucleases are site-specific endonucleases that cut both strands of double-stranded DNA upstream and downstream of their recognition sequences.
Arun S. Seetharam, Gary W. Stuart
doaj   +2 more sources

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

Mechanism of DNA Recognition by the Restriction Enzyme EcoRV

open access: yesJournal of Molecular Biology, 2010
EcoRV, a restriction enzyme in Escherichia coli, destroys invading foreign DNA by cleaving it at the center step of a GATATC sequence. In the EcoRV-cognate DNA crystallographic complex, a sharp kink of 50 degrees has been found at the center base-pair step (TA).
Zahran M   +3 more
openaire   +4 more sources

DNAtraffic--a new database for systems biology of DNA dynamics during the cell life. [PDF]

open access: yes, 2011
DNAtraffic (http://dnatraffic.ibb.waw.pl/) is dedicated to be a unique comprehensive and richly annotated database of genome dynamics during the cell life.
Barszcz, Daniela   +9 more
core   +1 more source

An alternative approach to study the enzymatic specificities of the CfrBI restriction–modification system

open access: yesHeliyon, 2019
Restriction–modification systems (RMS) are the main gene-engineering tools and a suitable model to study the molecular mechanisms of catalysis and DNA–protein interactions.
Marina V. Zakharova   +3 more
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

open access: yesFEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

open access: yesFEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee   +7 more
wiley   +1 more source

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