Results 81 to 90 of about 25,600,830 (293)

DeepSNVMiner: a sequence analysis tool to detect emergent, rare mutations in subsets of cell populations [PDF]

PeerJ, 2016
Background. Massively parallel sequencing technology is being used to sequence highly diverse populations of DNA such as that derived from heterogeneous cell mixtures containing both wild-type and disease-related states.
T. Daniel Andrews, Yogesh Jeelall, Dipti Talaulikar, Christopher C. Goodnow, Matthew A. Field   +4 more
doaj   +2 more sources

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Southern-by-Sequencing: A Robust Screening Approach for Molecular Characterization of Genetically Modified Crops

The Plant Genome, 2015
Molecular characterization of events is an integral part of the advancement process during genetically modified (GM) crop product development. Assessment of these events is traditionally accomplished by polymerase chain reaction (PCR) and Southern blot ...
Gina M. Zastrow-Hayes, Haining Lin, Amy L. Sigmund, Jenna L. Hoffman, Clara M. Alarcon, Kevin R. Hayes, Todd A. Richmond, Jeffery A. Jeddeloh, Gregory D. May, Mary K. Beatty   +9 more
doaj   +1 more source

Genome sequencing data for wild and cultivated bananas, plantains and abacá

Data in Brief, 2020
We performed shotgun genome sequencing on a total of 19 different Musa genotypes including representatives of wild banana species Musa acuminata and M. balibisiana, allopolyploid bananas and plantains, Fe'i banana, pink banana (also known as hairy banana)
Christine Sambles, Lakshmipriya Venkatesan, Olanrewaju M. Shittu, James Harrison, Karen Moore, Leena Tripathi, Murray Grant, Rachel Warmington, David J. Studholme   +8 more
doaj   +1 more source

Preferential access to genetic information from endogenous hominin ancient DNA and accurate quantitative SNP-typing via SPEX [PDF]

, 2009
The analysis of targeted genetic loci from ancient, forensic and clinical samples is usually built upon polymerase chain reaction (PCR)-generated sequence data.
Endicott, Phillip, Sanchez, J., Cooper, Alan, Endicott, P., Brotherton, Paul, Sanchez, Juan J, Brotherton, P., Sanchez, Juan J., Cooper, A.   +8 more
core   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Molecular characterization of Colletotrichum species causing Begonia anthracnose in Sri Lanka

Ceylon Journal of Science, 2020
Anthracnose disease is known to affect many tropical and subtropical fruits, vegetables and also certain cut-flowers and foliage plants. The disease was known to be caused by Colletotrichum gloeosporioides or C.
P. Wickramasinghe, N. Adikaram, D. Yakandawala   +2 more
doaj   +1 more source

DNA methylation assessed by SMRT sequencing is linked to mutations in Neisseria meningitidis isolates [PDF]

, 2015
The Gram-negative bacterium Neisseria meningitidis features extensive genetic variability. To present, proposed virulence genotypes are also detected in isolates from asymptomatic carriers, indicating more complex mechanisms underlying variable ...
Gerd Pluschke, Christoph D. Schmid (72046), Schmid, Christoph D., Mane, Shrikant, Korlach, Jonas, Tyson A. Clark (105783), Katharina Röltgen, Wang, Guilin, Araceli Lamelas, Jonas Korlach (105788), Shrikant Mane, Araceli Lamelas (128475), Christoph D Schmid, Mohamad R Abdul Sater, Clark, Tyson A., Röltgen, Katharina, Gerd Pluschke (7855), Katharina Röltgen (120045), Guilin Wang, Sater, Mohamad R. Abdul, Tyson A Clark, Mohamad R. Abdul Sater (836669), Jonas Korlach, Guilin Wang (37620), Shrikant Mane (106631), Lamelas, Araceli, Pluschke, Gerd   +26 more
core   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source