Results 241 to 250 of about 2,166,198 (276)
Some of the next articles are maybe not open access.
Related searches:
Related searches:
Current Protocols in Molecular Biology, 2006
AbstractPolony DNA sequencing provides an inexpensive, accurate, high‐throughput way to resequence genomes of interest by comparison to a reference genome. Mate‐paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on
Gregory J, Porreca +2 more
openaire +2 more sources
AbstractPolony DNA sequencing provides an inexpensive, accurate, high‐throughput way to resequence genomes of interest by comparison to a reference genome. Mate‐paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on
Gregory J, Porreca +2 more
openaire +2 more sources
Nature Methods, 2021
Loop-seq is a high-throughput sequencing assay that measures DNA looping and can help explain how DNA bendability contributes to nucleosome organization.
openaire +2 more sources
Loop-seq is a high-throughput sequencing assay that measures DNA looping and can help explain how DNA bendability contributes to nucleosome organization.
openaire +2 more sources
1988
DNA sequencing is a technique that is routinely performed in many molecular biology laboratories. This paper will describe some of the advances in DNA sequencing spanning the past ten years. With current manual sequencing techniques, it is possible for an average researcher to sequence 10100,000 bases per year.
M A, Kashdan, G L, Trainor
openaire +2 more sources
DNA sequencing is a technique that is routinely performed in many molecular biology laboratories. This paper will describe some of the advances in DNA sequencing spanning the past ten years. With current manual sequencing techniques, it is possible for an average researcher to sequence 10100,000 bases per year.
M A, Kashdan, G L, Trainor
openaire +2 more sources
Science, 1988
The increasing demand for DNA sequences can be met by replacement of each DNA sample in a device with a mixture of N samples so that the normal throughput is increased by a factor of N . Such a method is described.
G M, Church, S, Kieffer-Higgins
openaire +2 more sources
The increasing demand for DNA sequences can be met by replacement of each DNA sample in a device with a mixture of N samples so that the normal throughput is increased by a factor of N . Such a method is described.
G M, Church, S, Kieffer-Higgins
openaire +2 more sources
Characteristic Sequences for DNA Primary Sequence
Journal of Chemical Information and Computer Sciences, 2002A DNA sequence can be identified with a word over an alphabet N = [A, C, G, T]. Characteristic sequences of a DNA sequence are given in term of classifications of bases of nucleic acids. Using the characteristic sequences, we construct a set of 2 x 2 matrices to represent DNA primary sequences, which are based on counting of the frequency of occurrence
Ping-an, He, Jun, Wang
openaire +2 more sources
DNA Sequence Analysis and Comparative Sequencing
Methods, 1998Abstract This article aims to give an introduction to comparative sequencing and analysis, containing a brief section on sequence production, but focusing on the computer-based ( in silico ) analysis of genomic sequence.
A M, Mallon, M, Strivens
openaire +2 more sources
2010
Fluorescent cycle sequencing of PCR products is a multistage process and several methodologies are available to perform each stage. This chapter will describe the more commonly utilised dye-terminator cycle sequencing approach using BigDye® terminator chemistry (Applied Biosystems) ready for analysis on a 3730 DNA genetic analyzer.
Yvonne, Wallis, Natalie, Morrell
openaire +2 more sources
Fluorescent cycle sequencing of PCR products is a multistage process and several methodologies are available to perform each stage. This chapter will describe the more commonly utilised dye-terminator cycle sequencing approach using BigDye® terminator chemistry (Applied Biosystems) ready for analysis on a 3730 DNA genetic analyzer.
Yvonne, Wallis, Natalie, Morrell
openaire +2 more sources
2003
The polymerase chain reaction (PCR), first published by Mullis and Faloona in 1987 (1), has become an invaluable molecular biology tool in both research and routine applications. The combination of PCR with the chain-termination sequencing technique developed by Sanger et al.
openaire +2 more sources
The polymerase chain reaction (PCR), first published by Mullis and Faloona in 1987 (1), has become an invaluable molecular biology tool in both research and routine applications. The combination of PCR with the chain-termination sequencing technique developed by Sanger et al.
openaire +2 more sources
2013
This chapter looks at DNA sequencing, a technique used to determine the precise order of the nucleotide bases-adenine, guanine, cytosine, and thymine-in a DNA fragment. The chapter explains the first-generation sequencing methods: the chemical method introduced by Maxam and Gilbert in 1977, the Sanger dideoxy-chain-termination sequencing method, and ...
Tania Slatter, Alison Fitches
openaire +2 more sources
This chapter looks at DNA sequencing, a technique used to determine the precise order of the nucleotide bases-adenine, guanine, cytosine, and thymine-in a DNA fragment. The chapter explains the first-generation sequencing methods: the chemical method introduced by Maxam and Gilbert in 1977, the Sanger dideoxy-chain-termination sequencing method, and ...
Tania Slatter, Alison Fitches
openaire +2 more sources