Results 1 to 10 of about 3,728,691 (365)
DNA sequencing with chain-terminating inhibitors [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 1977 A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol.
Frederick Sanger +2 more
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Journal of Clinical and Translational Science, 2022 OBJECTIVES/GOALS: Age-specific N-glycosylation occurs on follicle- stimulating hormone (FSH) in pituitaries of post-menopausal women and results in a higher ratio of fully glycosylated to hypoglycosylated FSH.
Rosemary McDonald +4 more
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DNA 5-methylcytosine detection and methylation phasing using PacBio circular consensus sequencing
bioRxiv, 2023 Long single-molecular sequencing, such as PacBio circular consensus sequencing (CCS) and nanopore sequencing, is advantageous in detecting DNA 5-methylcytosine (5mC) in CpGs, especially in repetitive genomic regions.
Peng Ni +12 more
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Long-Read DNA Sequencing: Recent Advances and Remaining Challenges.
Annual review of genomics and human genetics (Print), 2023 DNA sequencing has revolutionized medicine over recent decades. However, analysis of large structural variation and repetitive DNA, a hallmark of human genomes, has been limited by short-read technology, with read lengths of 100-300 bp.
P. Warburton, R. Sebra
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Proceedings of the National Academy of Sciences of the United States of America, 1992 Marianne Frommer +7 more
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Molecular Neurodegeneration, 2021 Background Previous studies have reported that gut microbiota, permeability, short-chain fatty acids (SCFAs), and inflammation are altered in Parkinson’s disease (PD), but how these factors are linked and how they contribute to disease processes and ...
Velma T. E. Aho +9 more
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Molecular Therapy: Methods & Clinical Development, 2022 With the aim of expediting drug target discovery and validation for respiratory diseases, we developed an optimized method for in situ somatic gene disruption in murine lung epithelial cells via AAV6-mediated CRISPR-Cas9 delivery.
Honglin Chen +13 more
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BMC Genomic Data, 2023 Background Streptococcus pneumoniae (pneumococcus) represents an important human pathogen, responsible for respiratory and invasive infections in the community.
Weronika Puzia +4 more
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Multiomics implicate gut microbiota in altered lipid and energy metabolism in Parkinson’s disease
npj Parkinson's Disease, 2022 We aimed to investigate the link between serum metabolites, gut bacterial community composition, and clinical variables in Parkinson’s disease (PD) and healthy control subjects (HC).
Pedro A. B. Pereira +6 more
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Sequencing DNA with nanopores: Troubles and biases
PLoS ONE, 2021 Oxford Nanopore Technologies’ (ONT) long read sequencers offer access to longer DNA fragments than previous sequencer generations, at the cost of a higher error rate. While many papers have studied read correction methods, few have addressed the detailed
Clara Delahaye, J. Nicolas
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