Results 241 to 250 of about 3,728,691 (365)

Characterization of WAC interactions with R2TP and TTT chaperone complexes linking glucose and glutamine availability to mTORC1 activity

FEBS Open Bio, EarlyView.
TTT and R2TP chaperone complexes are required for the assembly and activation of mTORC1. WAC directly interacts with components of TTT, R2TP, and mTORC1, and these interactions are affected by the availability of glucose and glutamine, correlating with changes in mTORC1 activity.
Sofía Cabezudo, Natalia Cuervo, Carmen García‐Martín, Andrés López‐Perrote, Clara Reglero, Adrián Maqueda‐Real, Ana González‐Corpas, Marina Serna, Diego Megías, Alejo Efeyan, Solip Park, Oscar Llorca   +11 more
wiley   +1 more source

Precise measurement of CRISPR genome editing outcomes through single-cell DNA sequencing. [PDF]

Mol Ther Methods Clin Dev
Kalter N, Gulati S, Rosenberg M, Ayaz Q, Nguyen J, Wang S, Schroeder B, Li CY, Hendel A.   +8 more
europepmc   +1 more source

A nonsense mutation causing decreased levels of insulin receptor mRNA: detection by a simplified technique for direct sequencing of genomic DNA amplified by the polymerase chain reaction.

, 1990
Takashi Kadowaki, Hiroaki Kadowaki, Simeon I. Taylor   +2 more
openalex   +1 more source

Two‐way inhibition of PAX5 transcriptional activity by PAX5::CBFA2T3

FEBS Open Bio, EarlyView.
PAX5::CBFA2T3 (PAX5‐C) is a fusion protein of the B‐cell transcription factor, PAX5, and is found in B‐cell ALL. We propose a putative model of two‐way inhibition of PAX5 transcriptional activity by PAX5‐C. There are two ways of repression by PAX5‐C: DNA‐binding‐dependent way and HDAC‐dependent way, with either being sufficient for the repression. HDAC
Reina Ueno, Aki Terasaki, Yuiko Imai, Yuri Kimura, Yuna Kojima, Misa Irie, Koya Odaira, Mina Noura, Shuichi Okamoto, Takahiko Yasuda, Shinobu Tsuzuki, Hitoshi Kiyoi, Fumihiko Hayakawa   +12 more
wiley   +1 more source

High‐Throughput DNA sequencing of ancient wood

Molecular Ecology, 2018
S. Wagner, Frédéric Lagane, Andaine Seguin-Orlando, Mikkel Schubert, Thibault Leroy, E. Guichoux, É. Chancerel, Inger Bech-Hebelstrup, Vincent Bernard, Cyrille Billard, Yves Billaud, M. Bolliger, Christophe Croutsch, K. Čufar, F. Eynaud, K. Heussner, Joachim Köninger, Fabien Langenegger, F. Leroy, Christine Lima, N. Martinelli, G. Momber, A. Billamboz, O. Nelle, A. Palomo, R. Piqué, Marianne Ramstein, Roswitha Schweichel, H. Stäuble, Willy Tegel, X. Terradas, Florence Verdin, C. Plomion, A. Kremer, L. Orlando   +34 more
semanticscholar   +1 more source

An optimized protocol to detect high‐throughput DNA methylation from custom targeted sequences on 96 samples simultaneously

FEBS Open Bio, EarlyView.
Workflow of a high‐throughput technology for epigenotyping of differentially methylated CpGs in specific regions of the genome. The protocol works with small amounts of DNA extracted from blood or semen. The protocol consists of both enzymatic conversion of unmethylated cytosines and capture by hybridization with a custom panel.
Nathalie Iannuccelli, Sophie Valière, Julien Sarry, Cécile Donnadieu, Julie Demars   +4 more
wiley   +1 more source

Culture-negative liver abscess identified with plasma microbial cell-free DNA sequencing: A case report. [PDF]

Infect Med (Beijing)
Xue Y, Cerqueira FM, Stevenson HL, Williams-Bouyer N, Fang R.   +4 more
europepmc   +1 more source

High-resolution, strand-specific R-loop mapping via S9.6-based DNA–RNA immunoprecipitation and high-throughput sequencing

Nature Protocols, 2019
L. Sanz, F. Chédin
semanticscholar   +1 more source

The cochaperone BAG3 promotes the stabilization of p53 under heat stress conditions

FEBS Open Bio, EarlyView.
Under heat stress, BAG3 translocates to the nucleus and forms a complex with Hsp70 and p53, thereby promoting p53 stabilization and enhancing its transcriptional activity. These findings suggest that BAG3 functions as a cochaperone that supports p53‐mediated stress responses in cooperation with Hsp70.
Ngoc Nguyen Thi Minh, Esther Lee, Soo‐A Kim   +2 more
wiley   +1 more source

Ultra-low-input cell-free DNA sequencing for tumor detection and characterization in a real-world pediatric brain tumor cohort. [PDF]

Acta Neuropathol Commun
Fischer TT, Maaß KK, Puranachot P, Mieskolainen M, Sill M, Schad PS, Volz S, Rosing F, Wedig T, Schwarz N, Finster AME, Iser F, Meyer J, Sahm F, Lohi O, Damaty AE, Brors B, Haapasalo H, Pfister SM, Haapasalo J, Pajtler KW, Nordfors K.   +21 more
europepmc   +1 more source