Results 261 to 270 of about 1,871,571 (343)

High-Resolution Genetic Profiling of Hb J-Meerut and Other Hemoglobin Variants in the Tharu Population via HPLC and DNA Sequencing. [PDF]

Diagnostics (Basel)
Nigam N, Kushwaha R, Gupta A, Bhatt MLB, Singh B, Nigam S, Upadhyay K, Amara A, Rungta S.   +8 more
europepmc   +1 more source

Naked cuticle is essential for Drosophila wing development beyond Wingless signaling

FEBS Open Bio, EarlyView.
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley   +1 more source

NGS Technique for Palindromic Sequencing of DNA Through Effective PST-PCR

P Saraswat
openalex   +2 more sources

A standardized framework for robust fragmentomic feature extraction from cell-free DNA sequencing data. [PDF]

Genome Biol
Wang H, Mennea PD, Chan YKE, Cheng Z, Neofytou MC, Surani AA, Vijayaraghavan A, Ditter EJ, Bowers R, Eldridge MD, Shcherbo DS, Smith CG, Markowetz F, Cooper WN, Kaplan T, Rosenfeld N, Zhao H.   +16 more
europepmc   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Quantifying metabolites using structure-switching aptamers coupled to DNA sequencing. [PDF]

Nat Biotechnol
Tan JH, Fraser AG.
europepmc   +1 more source

Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe

FEBS Open Bio, EarlyView.
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley   +1 more source

High-performance protocol for ultra-short DNA sequencing using Oxford Nanopore Technology (ONT). [PDF]

PLoS One
Žemaitis L, Palepšienė R, Juzėnas S, Alzbutas G, Burgi PY, Heinis T, Charmet J, Angeloni Suter S, Jost M, Raišutis R, Simmel F, Galminas I.   +11 more
europepmc   +1 more source

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source

DDX3X induces mesenchymal transition of endothelial cells by disrupting BMPR2 signaling

FEBS Open Bio, EarlyView.
Elevated DDX3X expression led to downregulation of BMPR2, a key regulator of endothelial homeostasis and function. Our co‐immunoprecipitation assays further demonstrated a molecular interaction between DDX3X and BMPR2. Notably, DDX3X promoted lysosomal degradation of BMPR2, thereby impairing its downstream signaling and facilitating endothelial‐to ...
Yu Zhang, Jing Wang, De‐Hui Qian, Yang‐Fan Lv, Tian‐Le Cheng, Da‐Peng Wang, Jing Zhang, Ye Fan   +7 more
wiley   +1 more source