Results 281 to 290 of about 3,728,691 (365)

A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms

FEBS Open Bio, EarlyView.
We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley   +1 more source

The role of microbial cell free DNA sequencing in sepsis detection in the neonate. [PDF]

Pediatr Res
Toldi G, Majid A.
europepmc   +1 more source

HSP70 governs permeability and mechanotransduction in primary human endothelial cells

FEBS Open Bio, EarlyView.
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez, Everton G. Melo, Isadora C. B. Pavan, Percíllia V. S. Oliveira, Luiza B. C. T. Coimbra, Thaís L. S. Araujo   +5 more
wiley   +1 more source

Whole Mitochondrial DNA Sequencing Using Fecal Samples from Domestic Dogs. [PDF]

Animals (Basel)
Sugasawa T, Nguyen KDM, Otani N, Maehara K, Kamiya F, Hirokawa A, Takemasa T, Watanabe K, Nishi T, Sato K, Shimmura S, Takahashi Y, Kanki Y.   +12 more
europepmc   +1 more source

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

A regression based approach to phylogenetic reconstruction from multi-sample bulk DNA sequencing of tumors. [PDF]

PLoS Comput Biol
Schmidt H, Raphael BJ.
europepmc   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Rapid detection of rifampin resistance in <i>Mycobacterium tuberculosis</i> using nucleotide MALDI-TOF MS: a comparative study with phenotypic drug susceptibility testing and DNA sequencing. [PDF]

Microbiol Spectr
Zhang J, Zhang H, Wang J, Sun W, Liang Y, Yang Y, Ma Q, Wu X.   +7 more
europepmc   +1 more source

Retrospective analysis of 300 microbial cell-free DNA sequencing results in routine blood stream infection diagnostics. [PDF]

Front Cell Infect Microbiol
Neidhöfer C, Klein N, Yürüktümen A, Hattenhauer T, Mispelbaum R, Bode C, Holderried TAW, Hoerauf A, Parčina M.   +8 more
europepmc   +1 more source