Results 291 to 300 of about 1,188,878 (324)
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Aligning a DNA sequence with a protein sequence
Proceedings of the first annual international conference on Computational molecular biology - RECOMB '97, 1997We develop several algorithms for the problem of aligning DNA sequence with a protein sequence. Our methods account for frameshift errors, but not for introns in the DNA sequence. Thus, they are particularly appropriate for comparing a cDNA sequence that suffers from sequencing errors with an amino acid sequence or a protein sequence database.
Zheng Zhang +2 more
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Characteristic Sequences for DNA Primary Sequence
Journal of Chemical Information and Computer Sciences, 2002A DNA sequence can be identified with a word over an alphabet N = [A, C, G, T]. Characteristic sequences of a DNA sequence are given in term of classifications of bases of nucleic acids. Using the characteristic sequences, we construct a set of 2 x 2 matrices to represent DNA primary sequences, which are based on counting of the frequency of occurrence
Jun Wang, Ping-an He
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Analytical Biochemistry, 1988
A modification of Lin's systematic DNA sequencing strategy is described. A method based on the religation of compatible cohesive ends generated by Sau3AI and BamHI was developed. The original procedure has been simplified and the yield of transfectant has been greatly improved.
Yu-May Lee, Sheng-Chung Lee
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A modification of Lin's systematic DNA sequencing strategy is described. A method based on the religation of compatible cohesive ends generated by Sau3AI and BamHI was developed. The original procedure has been simplified and the yield of transfectant has been greatly improved.
Yu-May Lee, Sheng-Chung Lee
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Sequencing DNA bendability [PDF]
Nature Methods, 2021 Loop-seq is a high-throughput sequencing assay that measures DNA looping and can help explain how DNA bendability contributes to nucleosome organization.
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Multiplex DNA Sequencing [PDF]
Science, 1988 The increasing demand for DNA sequences can be met by replacement of each DNA sample in a device with a mixture of N samples so that the normal throughput is increased by a factor of N . Such a method is described.
Stephen Kieffer-Higgins +1 more
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1988
DNA sequencing is a technique that is routinely performed in many molecular biology laboratories. This paper will describe some of the advances in DNA sequencing spanning the past ten years. With current manual sequencing techniques, it is possible for an average researcher to sequence 10100,000 bases per year.
Maurice A. Kashdan, George L. Trainor
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DNA sequencing is a technique that is routinely performed in many molecular biology laboratories. This paper will describe some of the advances in DNA sequencing spanning the past ten years. With current manual sequencing techniques, it is possible for an average researcher to sequence 10100,000 bases per year.
Maurice A. Kashdan, George L. Trainor
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DNA Sequencing with Transposons
Journal of Computational Biology, 2000The use of transposons offers the possibility of a directed approach to DNA sequencing, where a target DNA up to about 6kb in length can be sequenced quickly and with minimal redundancy. Transposons are mobile DNA elements which can be inserted in a reasonably random fashion into the target DNA.
Terence P. Speed, Simon Cawley
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The Future of DNA Sequencing [PDF]
Science, 1993 This article describes the emerging technologies for rapid sequencing of DNA. Technologies discussed include the use of ultrathin gels, replaceable or reusable gels, automated systems, new types of primers to replace the current shotgun method of DNA sequencing, mass spectrometric direct sequencing technologies, and sequencing by hybridization.
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Current Protocols in Molecular Biology, 2006
AbstractPolony DNA sequencing provides an inexpensive, accurate, high‐throughput way to resequence genomes of interest by comparison to a reference genome. Mate‐paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on
George M. Church +2 more
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AbstractPolony DNA sequencing provides an inexpensive, accurate, high‐throughput way to resequence genomes of interest by comparison to a reference genome. Mate‐paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on
George M. Church +2 more
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Analytical Chemistry, 1995
Faster methods than are currently available will be needed to sequence the billions of base pairs of DNA in the human genome in a reasonable number of years and without exhausting available funds. A new sequencing technology in which DNA binds or hybridizes' to an array of oligonucleotides on a silicon chip appears to be promising as a high-throughput ...
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Faster methods than are currently available will be needed to sequence the billions of base pairs of DNA in the human genome in a reasonable number of years and without exhausting available funds. A new sequencing technology in which DNA binds or hybridizes' to an array of oligonucleotides on a silicon chip appears to be promising as a high-throughput ...
openaire +3 more sources