Results 291 to 300 of about 1,871,571 (343)
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1988
DNA sequencing is a technique that is routinely performed in many molecular biology laboratories. This paper will describe some of the advances in DNA sequencing spanning the past ten years. With current manual sequencing techniques, it is possible for an average researcher to sequence 10100,000 bases per year.
M A, Kashdan, G L, Trainor
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DNA sequencing is a technique that is routinely performed in many molecular biology laboratories. This paper will describe some of the advances in DNA sequencing spanning the past ten years. With current manual sequencing techniques, it is possible for an average researcher to sequence 10100,000 bases per year.
M A, Kashdan, G L, Trainor
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Science, 1988
The increasing demand for DNA sequences can be met by replacement of each DNA sample in a device with a mixture of N samples so that the normal throughput is increased by a factor of N . Such a method is described.
G M, Church, S, Kieffer-Higgins
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The increasing demand for DNA sequences can be met by replacement of each DNA sample in a device with a mixture of N samples so that the normal throughput is increased by a factor of N . Such a method is described.
G M, Church, S, Kieffer-Higgins
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Characteristic Sequences for DNA Primary Sequence
Journal of Chemical Information and Computer Sciences, 2002A DNA sequence can be identified with a word over an alphabet N = [A, C, G, T]. Characteristic sequences of a DNA sequence are given in term of classifications of bases of nucleic acids. Using the characteristic sequences, we construct a set of 2 x 2 matrices to represent DNA primary sequences, which are based on counting of the frequency of occurrence
Ping-an, He, Jun, Wang
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DNA Sequence Analysis and Comparative Sequencing
Methods, 1998Abstract This article aims to give an introduction to comparative sequencing and analysis, containing a brief section on sequence production, but focusing on the computer-based ( in silico ) analysis of genomic sequence.
A M, Mallon, M, Strivens
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2010
Fluorescent cycle sequencing of PCR products is a multistage process and several methodologies are available to perform each stage. This chapter will describe the more commonly utilised dye-terminator cycle sequencing approach using BigDye® terminator chemistry (Applied Biosystems) ready for analysis on a 3730 DNA genetic analyzer.
Yvonne, Wallis, Natalie, Morrell
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Fluorescent cycle sequencing of PCR products is a multistage process and several methodologies are available to perform each stage. This chapter will describe the more commonly utilised dye-terminator cycle sequencing approach using BigDye® terminator chemistry (Applied Biosystems) ready for analysis on a 3730 DNA genetic analyzer.
Yvonne, Wallis, Natalie, Morrell
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2003
The polymerase chain reaction (PCR), first published by Mullis and Faloona in 1987 (1), has become an invaluable molecular biology tool in both research and routine applications. The combination of PCR with the chain-termination sequencing technique developed by Sanger et al.
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The polymerase chain reaction (PCR), first published by Mullis and Faloona in 1987 (1), has become an invaluable molecular biology tool in both research and routine applications. The combination of PCR with the chain-termination sequencing technique developed by Sanger et al.
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2013
This chapter looks at DNA sequencing, a technique used to determine the precise order of the nucleotide bases-adenine, guanine, cytosine, and thymine-in a DNA fragment. The chapter explains the first-generation sequencing methods: the chemical method introduced by Maxam and Gilbert in 1977, the Sanger dideoxy-chain-termination sequencing method, and ...
Tania Slatter, Alison Fitches
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This chapter looks at DNA sequencing, a technique used to determine the precise order of the nucleotide bases-adenine, guanine, cytosine, and thymine-in a DNA fragment. The chapter explains the first-generation sequencing methods: the chemical method introduced by Maxam and Gilbert in 1977, the Sanger dideoxy-chain-termination sequencing method, and ...
Tania Slatter, Alison Fitches
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Sequence‐dependent DNA structure
BioEssays, 1993AbstractThe three‐dimensional structure of DNA depends subtly on its sequence, and this property is used by the proteins that regulate gene expression to locate their target sequences. Despite the large body of experimental data that has been accumulated on the relationship between sequence and DNA structure, we still do not fully understand the ...
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DNA sequencing and helix–coil transition. III. DNA sequencing
Biopolymers, 1980AbstractWe show that the fine oscillatory structure of the DNA melting curve can be used to determine explicitly the nucleotide composition and the order of certain domains within the DNA. If DNA is specifically fragmented, the order of fragments can be learned directly from a comparison of the differential melting curves of the nonfragmented and ...
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2018
Abstract The emergence of eDNA analysis is tightly linked to the development of next-generation sequencing. Chapter 7 “DNA sequencing” gives an overview of the characteristics and limitations of the main next-generation sequencing platforms. It focuses particularly on the Illumina platform, which is the only technology currently suitable
Pierre Taberlet +3 more
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Abstract The emergence of eDNA analysis is tightly linked to the development of next-generation sequencing. Chapter 7 “DNA sequencing” gives an overview of the characteristics and limitations of the main next-generation sequencing platforms. It focuses particularly on the Illumina platform, which is the only technology currently suitable
Pierre Taberlet +3 more
openaire +1 more source