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Independent genetic basis of meiotic crossover positioning and interference in domestic pigs. [PDF]
Sci RepBrekke C +3 more
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inMOTIFin: a lightweight end-to-end simulation software for regulatory sequences. [PDF]
BioinformaticsFerenc K +4 more
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Consumed by Abdominal Distention
Arthritis Care &Research, EarlyView.
Abimbola Fadairo‐Azinge +3 more
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Single-Stranded DNA Family Shuffling
2004Publisher Summary This chapter examines the various aspects of single-stranded DNA family shuffling. DNA shuffling is a technique that allows accelerated and directed protein evolution in vitro . This technique has been shown to be powerful in combining independently isolated mutations of a gene into a single progeny. By using the sense strand ssDNA
Osamu, Kagami +2 more
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Proceedings of the second annual international conference on Computational molecular biology - RECOMB '98, 1998
In vitro evolution is a new, important laboratory method to evolve molecules with desired properties. It has been used in a variety of biological studies and drug development. In this paper, we study one important mutagenesis method used in in vitro evolution experiments called DNA shuffling.
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In vitro evolution is a new, important laboratory method to evolve molecules with desired properties. It has been used in a variety of biological studies and drug development. In this paper, we study one important mutagenesis method used in in vitro evolution experiments called DNA shuffling.
openaire +2 more sources
RNA editing meets DNA shuffling
Nature, 2000The immune system can tailor its production of antibodies to the type of infection. Three sequential DNA-shuffling processes underlie this ability. The same protein is responsible for the last two of the processes, and oddly it is an RNA-editing rather than a DNA-recombining enzyme.
Ming Tian, Frederick W. Alt
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DNA Shuffling of Cytochrome P450 Enzymes
2013DNA family shuffling is an efficient method for creating libraries of novel enzymes, in which a high proportion of mutants exhibit correct folding and possess catalytic properties distinct from the starting material. The evolutionary arrangement of cytochromes P450 into subfamilies of enzymes with highly similar nucleotide sequences but distinct ...
Behrendorff, James B.Y.H. +2 more
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Artificial evolution by DNA shuffling
Trends in Biotechnology, 1998Improvement of enzymes is one of the important objectives of biotechnology. In vitro evolution of enzymes using DNA shuffling involves the assembly of two or more DNA segments into a full-length gene by homologous, or site-specific, recombination. Before the assembly, the segments are often subjected to random mutagenesis by error-prone PCR, random ...
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Science, 2001
The core technology behind directed molecular evolution is the polymerase chain reaction, the "molecular photocopier" that9s used to amplify small amounts of DNA. But scientists at one biotech that is using the technique to produce vaccines have given it a special tweak to produce what they call "DNA shuffling."
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The core technology behind directed molecular evolution is the polymerase chain reaction, the "molecular photocopier" that9s used to amplify small amounts of DNA. But scientists at one biotech that is using the technique to produce vaccines have given it a special tweak to produce what they call "DNA shuffling."
openaire +1 more source