Results 41 to 50 of about 12,600 (157)
PLoS Computational BiologyDNA supercoiling-the level of twisting and writhing of the DNA molecule around itself-plays an important role in the regulation of gene expression in bacteria by modulating promoter activity.
Théotime Grohens +2 more
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Topoisomerase IIα represses transcription by enforcing promoter-proximal pausing
Cell Reports, 2021 Summary: Accumulation of topological stress in the form of DNA supercoiling is inherent to the advance of RNA polymerase II (Pol II) and needs to be resolved by DNA topoisomerases to sustain productive transcriptional elongation.
Andrés Herrero-Ruiz +6 more
doaj +1 more source
Biotechnology and Applied Biochemistry, EarlyView.ABSTRACT Rolling‐circle amplification (RCA) is a completely synthetic and rapid mechanism for scaling‐up bulk DNA that eliminates requirements for large‐scale bacterial fermentation. Several reports have described the surprising ability of large unprocessed (hyperbranched) rolling circle‐amplified DNA (RCA DNA) to effectively transfect cultured cells ...
Evelina Loghin +8 more
wiley +1 more source
ERK2-topoisomerase II regulatory axis is important for gene activation in immediate early genes
Nature Communications, 2023 The function of the mitogen-activated protein kinase signaling pathway is required for the activation of immediate early genes (IEGs), including EGR1 and FOS, for cell growth and proliferation.
Heeyoun Bunch +10 more
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Chemistry – A European Journal, EarlyView.5‐Substituted 2‐(naphthalen‐2‐yl)‐4H‐chromen‐4‐ones are reported as a novel class of highly potent and selective triplex DNA ligands. These ligands induce triplex formation at submicromolar concentrations and inhibit enzymatic activity via ligand‐mediated triplex formation.
Nghia Tran +4 more
wiley +1 more source
Microorganisms, 2019 Prokaryotes have an essential gene—gyrase—that catalyzes negative supercoiling of plasmid and chromosomal DNA. Negative supercoils influence DNA replication, transcription, homologous recombination, site-specific recombination, genetic ...
Nikolay S. Rovinskiy +3 more
doaj +1 more source
Advances in Double‐Stranded DNA Targeting Technologies
Exploration, EarlyView.Double‐stranded DNA (dsDNA) plays a crucial role in genetic information storage and disease management, but its inherent stability limits access to internal bases. To overcome this challenge, various high‐specificity molecular targeting technologies have been developed to destabilize the DNA structure.
Zuhao Shen +8 more
wiley +1 more source
Dynamic interplay between target search and recognition for a Type I CRISPR-Cas system
Nature Communications, 2023 CRISPR-Cas effector complexes enable the defense against foreign nucleic acids and have recently been exploited as molecular tools for precise genome editing at a target locus. To bind and cleave their target, the CRISPR-Cas effectors have to interrogate
Pierre Aldag +8 more
doaj +1 more source
Macromolecular Rapid Communications, EarlyView.Guanidinium‐ and indole‐functionalized poly(glycidyl ether)s were developed as non‐viral gene delivery vectors with tunable hydrophilic–hydrophobic balance. All polymers formed stable DNA polyplexes, while moderate indole content yielded the highest transfection efficiency at low N/P ratios.
Markus Kötzsche +8 more
wiley +1 more source
Proteins: Structure, Function, and Bioinformatics, EarlyView.ABSTRACT Starch is the major energy storage compound in plants. It accumulates in the form of insoluble, partly crystalline granules whose number and shape are specific to each plant species. These characteristics are defined very early in starch biosynthesis, at the initiation stage.
Mélanie Bossu +5 more
wiley +1 more source