Results 31 to 40 of about 79,794 (267)

Machines on Genes: Enzymes that Make, Break and Move DNA and RNA [PDF]

, 2010
As the vital information repositories of the cell, the nucleic acids DNA and RNA pose many challenges as enzyme substrates. To produce, maintain and repair DNA and RNA, and to extract the genetic information that they encode, a battery of remarkable ...
Bates, Belousoff, Ben F. Luisi, Lilley, Marshall, Olorunniji, Rice, Richard P. Bowater, Sherratt, Smith, Szczelkun, Tomlinson, W. Marshall Stark, Weinzierl   +13 more
core   +1 more source

Kinetic Study of DNA Topoisomerases by Supercoiling-Dependent Fluorescence Quenching

ACS Omega, 2019
DNA topoisomerases are essential enzymes for all living organisms and important targets for anticancer drugs and antibiotics. Although DNA topoisomerases have been studied extensively, steady-state kinetics has not been systematically investigated ...
Yunke Wang, Samantha Rakela, J. Chambers, Z. Hua, M. Muller, J. Nitiss, Y. Tse‐Dinh, F. Leng   +7 more
semanticscholar   +1 more source

Topoisomerases I and III inhibit R-loop formation to prevent unregulated replication in the chromosomal Ter region of Escherichia coli. [PDF]

PLoS Genetics, 2018
Type 1A topoisomerases (topos) are the only ubiquitous topos. E. coli has two type 1A topos, topo I (topA) and topo III (topB). Topo I relaxes negative supercoiling in part to inhibit R-loop formation. To grow, topA mutants acquire compensatory mutations,
Julien Brochu, Émilie Vlachos-Breton, Sarah Sutherland, Makisha Martel, Marc Drolet   +4 more
doaj   +1 more source

Seconeolitsine, the Novel Inhibitor of DNA Topoisomerase I, Protects against Invasive Pneumococcal Disease Caused by Fluoroquinolone-Resistant Strains

Antibiotics, 2021
Antibiotic resistance in Streptococcus pneumoniae has increased worldwide, making fluoroquinolones an alternative therapeutic option. Fluoroquinolones inhibit the type II DNA topoisomerases (topoisomerase IV and gyrase).
Jose Manuel Tirado-Vélez, David Carreño, David Sevillano, Luis Alou, José Yuste, Adela G. de la Campa   +5 more
doaj   +1 more source

Atomic force microscopy shows that vaccinia topoisomerase IB generates filaments on DNA in a cooperative fashion [PDF]

, 2005
Type IB DNA topoisomerases cleave and rejoin one strand of the DNA duplex, allowing for the removal of supercoils generated during replication and transcription.
Dekker, Cees, Dekker, Nynke H., Holtzer, Laurent, Koster, Daniel A., Moreno-Herrero, Fernando, Shuman, Stewart   +5 more
core   +2 more sources

Distinct requirements for the Rad32(Mre¹¹) nuclease and Ctp1(CtIP) in the removal of covalently bound topoisomerase I and II from DNA [PDF]

, 2009
For a cancer cell to resist treatment with drugs that trap topoisomerases covalently on the DNA, the topoisomerase must be removed. In this study, we provide evidence that the Schizosaccharomyces pombe Rad32Mre11 nuclease activity is involved in the ...
Akamatsu, Antony M. Carr, Baldwin, Champoux, Connelly, Connelly, Edgar Hartsuiker, El-Khamisy, Farah, Gutz, Hartsuiker, Hopfner, Hopfner, Keeney, Keeney, Krogh, Krogh, Lengsfeld, Limbo, Liu, Malik, Matthew J. Neale, McKee, Miyoshi, Moreau, Neale, Nitiss, Osheroff, Porter, Pouliot, Prinz, Sartori, Shaw, Stracker, Subramanian, Takashima, Tomita, Utsugi, Young   +38 more
core   +2 more sources

Crystallization and preliminary crystallographic analysis of the DNA gyrase B protein from B-stearothermophilus [PDF]

, 1996
DNA gyrase B (GyrB) from B. stearothermophilus has been crystallized in the presence of the non-hydrolyzable ATP analogue, 5'-adenylpl-beta-gamma-imidodiphosphate (ADPNP), by the dialysis method.
Brannigan, J A, Subramanya, H S, Tsai, F T F, Wigley, D B, Wilkinson, A J   +4 more
core   +2 more sources

Structure of the human type I DNA topoisomerase gene

Journal of Biological Chemistry, 1991
We describe the molecular organization of the human gene coding for type I DNA topoisomerase. The coding sequence is split into 21 exons distributed over at least 85 kilobase pairs (kb) of human genomic DNA. The sizes of the 20 introns vary widely between 0.2 and at least 30 kb and all contain the sequence elements known to be required for pre-mRNA ...
N, Kunze, G C, Yang, M, Dölberg, R, Sundarp, R, Knippers, A, Richter   +5 more
openaire   +2 more sources

Camptothecin, A Specific Inhibitor of Type I DNA Topoisomerase, Induces DNA Breakage at Replication Forks [PDF]

Molecular and Cellular Biology, 1988
The structure of replicating simian virus 40 minichromosomes, extracted from camptothecin-treated infected cells, was investigated by biochemical and electron microscopic methods. We found that camptothecin frequently induced breaks at replication forks close to the replicative growth points.
K, Avemann, R, Knippers, T, Koller, J M, Sogo   +3 more
openaire   +2 more sources

Characterization of a pathway of genomic instability induced by R-loops and its regulation by topoisomerases in E. coli.

PLoS Genetics, 2023
The prototype enzymes of the ubiquitous type IA topoisomerases (topos) family are Escherichia coli topo I (topA) and topo III (topB). Topo I shows preference for relaxation of negative supercoiling and topo III for decatenation.
Julien Brochu, Émilie Vlachos-Breton, Dina Irsenco, Marc Drolet   +3 more
doaj   +1 more source