Results 71 to 80 of about 121,903 (271)
Advanced Science, EarlyView.This study presents the first telomere‐to‐telomere genomes and population resources for Diaporthe pathogens, uncovering the largest known fungal repertoire of secondary metabolite gene clusters. Structural variations and horizontal gene transfer drive cluster diversification, while specific rapidly evolving clusters control virulence, offering novel ...
Kainan Li +9 more
wiley +1 more source
Molecular Therapy: Methods & Clinical Development, 2021 The piggyBac transposon system provides a non-viral alternative for cost-efficient and simple chimeric antigen receptor (CAR) T cell production. The generation of clinical-grade CAR T cells requires strict adherence to current good manufacturing practice
Iva Kaštánková +7 more
doaj +1 more source
Characterization of transposon insertion mutants in desulfovibrio vulgaris hilderborough by sequencing genomic DNA [abstract] [PDF]
, 2007 only availableTn5 transposon mutagenesis occurs by a mechanism in which a segment of DNA (transposon) encoded in a plasmid is inserted into genomic DNA (the target) by a conservative (cut-and-paste) mechanism.
Giles, Barbara J. +2 more
core
Dissecting a complex chemical stress: chemogenomic profiling of plant hydrolysates. [PDF]
, 2013 The efficient production of biofuels from cellulosic feedstocks will require the efficient fermentation of the sugars in hydrolyzed plant material.
Arkin, Adam P +14 more
core +2 more sources
Discovery of H2 Receptor Antagonists as Colistin Enhancers by Targeting Acid Stress Response
Advanced Science, EarlyView.This study identifies YqgB as a key target for restoring colistin susceptibility in mcr‐positive pathogens under acidic conditions by remodeling phospholipid composition and reducing LPS modification. Deep learning‐based screening reveals H2 receptor antagonists as novel colistin adjuvants. Further investigations indicate that ranitidine and famotidine
Jinju Cai +7 more
wiley +1 more source
IS21 family transposase cleaved donor complex traps two right-handed superhelical crossings
Nature Communications, 2023 Transposases are ubiquitous enzymes that catalyze DNA rearrangement events with broad impacts on gene expression, genome evolution, and the spread of drug-resistance in bacteria.
Mercedes Spínola-Amilibia +4 more
doaj +1 more source
Reduced mutation rate and increased transformability of transposon-free Acinetobacter baylyi ADP1-ISx [PDF]
, 2017 The genomes of most bacteria contain mobile DNA elements that can contribute to undesirable genetic instability in engineered cells. In particular, transposable insertion sequence (IS) elements can rapidly inactivate genes that are important for a ...
Barrick, Jeffrey E +3 more
core +2 more sources
Advanced Science, EarlyView.In this study, the orange‐muscle giant abalone (Haliotis gigantea) is used as a model to identify a non‐coding SNP that disrupts the interaction between ITGA8 pre‐mRNA and the splicing factor ILF2, leading to altered ITGA8 splicing. These splicing changes promote carotenoid accumulation in abalone muscle through the regulation of tissue remodeling ...
Xiaohui Wei +17 more
wiley +1 more source
T Cell Exhaustion in Cancer Immunotherapy: Heterogeneity, Mechanisms, and Therapeutic Opportunities
Advanced Science, EarlyView.T cell exhaustion limits immunotherapy efficacy. This article delineates its progression from stem‐like to terminally exhausted states, governed by persistent antigen, transcription factors, epigenetics, and metabolism. It maps the exhaustion landscape in the TME and proposes integrated reversal strategies, providing a translational roadmap to overcome
Yang Yu +7 more
wiley +1 more source
Bacterial N4-methylcytosine as an epigenetic mark in eukaryotic DNA
Nature Communications, 2022 Eukaryotic DNA can be methylated as 5-methylcytosine and N6-methyladenine, but whether other forms of DNA methylation occur has been controversial. Here the authors show that a bacterial DNA methyltransferase was acquired >60 Mya in bdelloid rotifers ...
Fernando Rodriguez +3 more
doaj +1 more source