Primed CRISPR DNA uptake in Pyrococcus furiosus. [PDF]
Nucleic Acids Res, 2020 AbstractCRISPR-Cas adaptive immune systems are used by prokaryotes to defend against invaders like viruses and other mobile genetic elements. Immune memories are stored in the form of ‘spacers’ which are short DNA sequences that are captured from invaders and added to the CRISPR array during a process called ‘adaptation’.
Garrett S +5 more
europepmc +4 more sources
Cellular Uptake of Tile-Assembled DNA Nanotubes [PDF]
Nanomaterials, 2014 DNA-based nanostructures have received great attention as molecular vehicles for cellular delivery of biomolecules and cancer drugs. Here, we report on the cellular uptake of tubule-like DNA tile-assembled nanostructures 27 nm in length and 8 nm in ...
Samet Kocabey +7 more
doaj +4 more sources
Uptake and trafficking of DNA in keratinocytes: evidence for DNA-binding proteins [PDF]
Gene Therapy, 2004 The skin is an interesting organ for human gene therapy due to accessibility, immunologic potential and synthesis capabilities. In this study, we attempted to visualize and measure the uptake of naked FITC-labeled plasmid by FACS analysis detecting up to 15% internalization in a dose- and time-dependent manner.
Ulrich R Hengge
exaly +3 more sources
Mechanisms of Transforming DNA Uptake to the Periplasm of Bacillus subtilis [PDF]
MBio, 2021 David Dubnau
exaly +2 more sources
ComEA is essential for the transfer of external DNA into the periplasm in naturally transformable Vibrio cholerae cells. [PDF]
PLoS Genetics, 2014 The DNA uptake of naturally competent bacteria has been attributed to the action of DNA uptake machineries resembling type IV pilus complexes. However, the protein(s) for pulling the DNA across the outer membrane of Gram-negative bacteria remain ...
Patrick Seitz +7 more
doaj +1 more source
Evolution of DNA Uptake Signal Sequences [PDF]
Artificial Life, 2005 The DNA of some naturally competent species of bacteria contains a large number of evenly distributed copies of a short sequence. This highly overrepresented sequence is believed to be an uptake signal sequence (USS) that helps bacteria to take up DNA selectively from (dead) members of their own species.
Dominique F. Chu +2 more
openaire +5 more sources
Midcell recruitment of the DNA uptake and virulence nuclease, EndA, for pneumococcal transformation. [PDF]
PLoS Pathogens, 2013 Genetic transformation, in which cells internalize exogenous DNA and integrate it into their chromosome, is widespread in the bacterial kingdom. It involves a specialized membrane-associated machinery for binding double-stranded (ds) DNA and uptake of ...
Matthieu J Bergé +5 more
doaj +1 more source
Programming cell entry of molecules via reversible synthetic DNA circuits on cell membrane
Fundamental Research, 2021 Cellular uptake of biomolecules is crucial for regulating cell function. However, powerful and biocompatible tools for dynamically manipulating the cell entry of single-stranded DNAs (ssDNAs) remain elusive.
Jiangbo Liu +6 more
doaj +1 more source
The Kinetics of DNA Uptake by Haemophilus influenzae [PDF]
Journal of General Microbiology, 1964 SUMMARY: The rates of formation of transformed cells in mixtures of transforming DNA and transformable Haemophilus influenzae bacteria have been measured. The data were interpreted as follows. About 4–5 sec. are required for the penetration of a DNA particle of 8–9 x 106 mean molecular weight.
J H, STUY, D, STERN
openaire +2 more sources
An intragenic distribution bias of DNA uptake sequences in
, 2008 Most sequenced strains from Pasteurellaceae and Neisseriae contain hundreds to thousands of uptake sequence (US) motifs in their genome, which are associated with natural competence for DNA uptake. The mechanism of their recognition is still unclear, and
van Passel Mark WJ +3 more
core +1 more source