Results 241 to 250 of about 2,477,957 (291)
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Nucleotide and DNA uptake by Neurospora crassa: Involvement of an uptake stimulating protein
Biochimica Et Biophysica Acta - Biomembranes, 1991The basal and DUSF (DNA-uptake-stimulating factor, described previously by Schablik and Szabó (1981) FEMS Microbiol. Lett. 10, 395-397) stimulated uptake of [3H]DNA and radioactive nucleotides by Neurospora crassa (FGSC 1118, slime) cell-wall-less strain was studied. The uptake of [3H]DNA by the cells is a saturable and time-dependent process.
Gabor Szabó, György Tóth
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Analysis of DNA uptake biases in bacteria with and without uptake specificity
, 2021 DNA uptake is the first step in natural transformation of bacteria, leading to DNA internalization and recombination. It is, therefore, a key determinant in genome evolution. Most bacteria take up DNA indiscriminately, but in Pasteurellaceae and Neisseriaceae, the uptake machinery binds preferentially to short sequences enriched in their genome, called
Mora Rodríguez, Marcelo Andrés
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Electrostimulated uptake of DNA by liposomes
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1990High molecular mass DNA was efficiently taken up by large unilamellar vesicles exposed to a short pulse of electric field (0.1-1 ms) with an intensity as high as 12.5 kV/cm. The efficiency of uptake increased significantly in presence of Mg2+ ions and was approximately 0.6 and 1.5 micrograms of DNA per mumol of lipid for T7 DNA and plasmid pBR 322 ...
L V, Chernomordik +2 more
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DNA uptake by Streptomyces species
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976Uptake of homologous and heterologous radioactively labelled DNA was studied in ten Streptomyces species. Among these, S. kasugaensis and S. virginiae were shown to take up 0.5-2.0% of the supplied donor DNA at a well-defined point in their growth cycle. The heterologous donor DNA taken up retained its original buoyant density in CsCl gradients.
P, Roelants +4 more
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Cellular Uptake of DNA Origami
2023This chapter discusses the methods involved in achieving and analyzing cellular uptake of DNA origami. While cells naturally internalize substances from their surroundings, more than a simple addition of DNA origami in the surrounding cell medium is necessary to ensure DNA origami particles successfully enter the intracellular environment.
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Uptake of exogenous DNA via the skin
Journal of Molecular Medicine, 1999Easy accessibility makes the skin extremely attractive for therapeutic gene transfer, but this feature may be equally responsible for inadvertent DNA uptake. Therefore we studied lacZ reporter gene expression after epicutaneous and intracutaneous administration of naked DNA, lipofection and transferrinfection to intact, tape-stripped, and wound-healing
A, Udvardi +4 more
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DNA uptake during bacterial transformation
Nature Reviews Microbiology, 2004Naturally competent bacteria are able to take up exogenous DNA and undergo genetic transformation. The transport of DNA from the extracellular milieu into the cytoplasm is a complex process, and requires proteins that are related to those involved in the assembly of type IV pili and type II secretion systems, as well as a DNA translocase complex at the
Inês, Chen, David, Dubnau
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Annual Review of Microbiology, 1999
▪ Abstract Natural competence is widespread among bacterial species. The mechanism of DNA uptake in both gram-positive and gram-negative bacteria is reviewed. The transformation pathways are discussed, with attention to the fate of donor DNA as it is processed by the competent cell.
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▪ Abstract Natural competence is widespread among bacterial species. The mechanism of DNA uptake in both gram-positive and gram-negative bacteria is reviewed. The transformation pathways are discussed, with attention to the fate of donor DNA as it is processed by the competent cell.
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Uptake of bacterial DNA by Chlamydomonas reinhardi
Mutation Research, 1975Escherichia coli [3H]DNA supplied to vegetative cultures of wild-type (mt+) and CW15 (mt+;mutant lacking the cell wall) Chlamydomonas reinhardi could bind to the cell wall of the wild-type and to the cell membrane of CW15 mutant cells. The extent of this binding decreased with time and was to a large degree (over 90%) DNA-ase-sensitive.
P F, Lurquin, R M, Behki
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Endocytosis is involved in DNA uptake in yeast
Biochimica et Biophysica Acta (BBA) - General Subjects, 2002The transfection of mammalian cells by endocytosis is frequently hampered by low efficiency. To identify the bottlenecks, a system that allows the analysis of the intracellular pathway of DNA along the endocytic compartments in the eukaryote Saccharomyces cerevisiae was developed.
Birgit, Neukamm +2 more
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