Results 251 to 260 of about 2,477,957 (291)

Uptake of DNA by Keratinocytes

2001
For genes to be expressed in skin and other tissues, a variety of different events have to occur. First, following intradermal injection of naked plasmid DNA, it has to be transported to the cells that potentially take it up. Most likely, physical pressure and the concentration gradient play an important role.
U. R. Hengge, E. Tschakarjan, A. Mirmohammdsadegh, M. Goos, H. E. Meyer   +4 more
openaire   +1 more source

The specific uptake of cloned Haemophilus DNA

Biochemical and Biophysical Research Communications, 1979
Abstract During the process of transformation Haemophilus influenzae cells bind its own DNA but little or no foreign DNA. This specificity for recognition of DNA was studied by cloning Haemophilus DNA in E. coli . Haemophilus DNA fragments were cloned using plasmid pBR322 as a vector.
B C, Chung, S H, Goodgal
openaire   +2 more sources

Regulation of Foreign DNA Uptake by Mouse Spermatozoa

Experimental Cell Research, 2001
We have studied some features of DNA uptake in both mature and immature mammalian spermatozoa. Mature sperm collected from the cauda epididymis are able to incorporate foreign DNA in a buffer containing only salts and calcium. Immature spermatozoa, however, are unable to bind DNA.
Carballada, Rosa, Esponda, Pedro
openaire   +3 more sources

The Mechanism of Naked DNA Uptake and Expression

2005
The administration of naked nucleic acids into animals is increasingly being used as a research tool to elucidate mechanisms of gene expression and the role of genes and their cognate proteins in the pathogenesis of disease in animal models (Herweijer and Wolff, 2003; Hodges and Scheule, 2003). It is also being used in several human clinical trials for
Jon A, Wolff, Vladimir, Budker
openaire   +2 more sources

Uptake of DNA by Living Cells

1965
Publisher Summary The chapter discusses the uptake of DNA, by living cells. The chapter describes the actual evidence concerning DNA uptake, by tissues and organs. The data obtained up to now encourage the hope that convincing demonstrations may ultimately be made. The biochemical data obtained, with two different analytical methods, point toward the
openaire   +2 more sources

Uptake and processing of DNA by Acinetobacter calcoaceticus – areview

Gene, 1997
In natural transformation, DNA in the form of macromolecular fragments can be translocated across the cell envelope of prokaryotic microorganisms. During the past two decades, several, largely mutually contradictory, hypotheses have been forwarded to explain the molecular mechanism and bioenergetics of this translocation process.
R, Palmen, K J, Hellingwerf
openaire   +2 more sources

Specificity in DNA uptake by gonococci

1988
Factors involved in the specificity of plasmid DNA uptake by competent gonococci were examined. Modification did not affect uptake, nor did plasmid conformation (linearized vs. circular). A 700 bp fragment from a 4.2 kb gonococcal cryptic plasmid was cloned into pLES2, a gonococcal-E.
K. L. Burnstein, P. F. Sparling
openaire   +1 more source

Uptake of DNA by competent bacteria—A possible mechanism

Journal of Theoretical Biology, 1969
At present there has been no satisfactory explanation of how such a large molecule as DNA can penetrate and transform a competent bacterium. A mechanism is proposed by which transforming DNA could be taken up by the mesosome organelles of Bacillus subtilis .
A, Akrigg, S R, Ayad, J, Blamire
openaire   +2 more sources

DNA Uptake by a Mutant Strain of Lymphoma Cells

Nature, 1968
A mutant line of the P388F lymphoma has been used as recipient for labelled DNA. A distinct peak of DNA resulted which was well separated from those given by donor and recipient DNA. This suggests true integration of DNA into the recipient genome.
Ayad, S R, Fox, M
openaire   +2 more sources

Endocytic uptake of fluorescence labelled DNA in yeast

Journal of Basic Microbiology, 2010
AbstractAfter dispiriting results using viral vectors in gene therapy, by which a number of patients acquired cancer as a result of the use of retroviral vector constructs, the percentage of non‐viral approaches has increased over recent years. To elucidate potential bottlenecks in the non‐viral transfection process we here introduce a novel method to ...
RIECHERS, Sean-Patrick Hermann, Stahl, Ulf, Lang, Christine   +2 more
openaire   +3 more sources