Gene transfer into hepatocytes using asialoglycoprotein receptor mediated endocytosis of DNA complexed with an artificial tetra-antennary galactose ligand [PDF]
, 1992 We have constructed an artificial ligand for the hepatocyte-specific asialoglycoprotein receptor for the purpose of generating a synthetic delivery system for DNA.
Zatloukal, Kurt +9 more
core +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
The Role of Marek’s Disease Virus UL12 and UL29 in DNA Recombination and the Virus Lifecycle
Viruses, 2019 Marek’s disease virus (MDV) is an oncogenic alphaherpesvirus that infects chickens and integrates its genome into the telomeres of latently infected cells.
Renato L. Previdelli +6 more
doaj +1 more source
The ubiquitin system in normal and infected germinal center B cells
FEBS Letters, EarlyView.The ubiquitin system plays a central role in germinal center (GC) B cells, influencing differentiation to long‐lived memory B cells. Oncogenic gammaherpesviruses gain access to memory B cells by establishing latency in GC B cells. Mapping ubiquitin mechanisms in normal and infected GC B cells will define specific molecular circuits in B cells germane ...
Destiny Davis +2 more
wiley +1 more source
Эпидемиология и вакцинопрофилактикаRelevance. Hepatitis B virus (HBV) is the etiologic agent of acute and chronic hepatitis B in humans. WHO recommends the use of sensitive laboratory assays based on nucleic acid amplification methods to detect HBV DNA.
M. A. Tyumentseva +3 more
doaj +1 more source
Molecular characterization of a tetra segmented ssDNA virus infecting Botrytis cinerea worldwide
Virology Journal, 2023 Background Family Genomoviridae was recently established, and only a few mycoviruses have been described and characterized, and almost all of them (Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1, Fusarium graminearum gemyptripvirus 1 and ...
Ana Ruiz-Padilla +2 more
doaj +1 more source
Development and evaluation of DNA vaccines in chickens against a wild bird H6N2 avian influenza virus from Western Australia [PDF]
, 2010 Genetic immunization, also known as DNA or polynucleotide immunisation, is well documented to induce broad-based immunity in various animal models of infectious and non-infectious diseases.
Shan, Songhua
core
Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy
FEBS Letters, EarlyView.The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley +1 more source
Cell-free (RNA) and cell-associated (DNA) HIV-1 and postnatal transmission through breastfeeding [PDF]
, 2012 <p>Introduction - Transmission through breastfeeding remains important for mother-to-child transmission (MTCT) in resource-limited settings. We quantify the relationship between cell-free (RNA) and cell-associated (DNA) shedding of HIV-1 virus in ...
Van de Perre, P +28 more
core +1 more source
Unlocking the potential of cell‐permeable nanobodies for intracellular applications
FEBS Letters, EarlyView.This review outlines current strategies for delivery of nanobodies into cells and highlights recent examples of functional applications. By addressing and manipulating intracellular targets, these molecules hold great potential for treating cancer, respiratory diseases and neurological disorders, making this a vibrant area of research in the biomedical
Mariona Gené‐Verdés +2 more
wiley +1 more source