Results 171 to 180 of about 8,074 (224)
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Designing in vivo active DNAzymes
Nature Chemistry, 2021The therapeutic applications of DNAzymes are limited because of their low effectiveness in vivo. Now, a promising approach for constructing DNAzymes that show high gene-silencing efficiency in mammalian cells has been developed. This approach incorporates chemical modifications into an existing DNAzyme scaffold.
Yifei Zhou, Chuanzheng Zhou
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DNAzyme-Based Microscale Thermophoresis Sensor
Analytical Chemistry, 2023Microscale thermophoresis (MST) technology has emerged as a powerful growing method in a molecular interaction study by measuring fluorescence responses of molecules inside a capillary to infrared (IR) laser heating with the benefits of rapid ratiometric measurement, separation-free, no immobilization, and low sample consumption.
Hao Yu, Qiang Zhao
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pH-programmable DNAzyme nanostructures
Chemical Communications, 2011Two engineered DNA nanostructures consisting of a nucleic acid functional hairpin and a DNA "tweezers" assembly act as pH-switchable devices for the "ON-OFF" activation/deactivation of the horseradish-peroxidase-mimicking DNAzyme.
Simcha, Shimron +3 more
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Chemical Communications, 2010
The activities of Mg(2+)-dependent DNAzymes are reversibly switched by ion stimuli using the thymine-Hg(2+)-thymine complexes or cytosine-Ag(+)-cytosine complexes.
Simcha, Shimron +3 more
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The activities of Mg(2+)-dependent DNAzymes are reversibly switched by ion stimuli using the thymine-Hg(2+)-thymine complexes or cytosine-Ag(+)-cytosine complexes.
Simcha, Shimron +3 more
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Biochemistry, 1997
A 33-nucleotide, guanine-rich DNA oligomer, PS5.ST1, has been reported to catalyze the metallation of mesoporphyrin IX (MPIX) by copper and zinc ions. In this paper we report a thorough investigation of the properties of this DNAzyme. We have established that a 24-nucleotide sequence (PS5.M), from within PS5.ST1, is both the minimal and most optimal ...
Y, Li, D, Sen
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A 33-nucleotide, guanine-rich DNA oligomer, PS5.ST1, has been reported to catalyze the metallation of mesoporphyrin IX (MPIX) by copper and zinc ions. In this paper we report a thorough investigation of the properties of this DNAzyme. We have established that a 24-nucleotide sequence (PS5.M), from within PS5.ST1, is both the minimal and most optimal ...
Y, Li, D, Sen
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DNAzyme–aptamer or aptamer–DNAzyme paradigm: Biochemical approach for aflatoxin analysis
Biotechnology and Applied Biochemistry, 2017AbstractDNAzyme and aptamer conjugations have already been used for sensitive and accurate detection of several molecules. In this study, we tested the relationship between conjugation orientation of DNAzyme and aflatoxin B1 aptamer and their subsequent peroxidase activity. Circular dichroism (CD) spectroscopy and biochemical analysis were used here to
Marzieh, Jafari +4 more
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Cleaving Folded RNA with DNAzyme Agents
ChemBioChem, 2023AbstractCleavage of biological mRNA by DNAzymes (Dz) has been proposed as a variation of oligonucleotide gene therapy (OGT). The design of Dz‐based OGT agents includes computational prediction of two RNA‐binding arms with low affinity (melting temperatures (Tm) close to the reaction temperature of 37 °C) to avoid product inhibition and maintain high ...
Daria D. Nedorezova +7 more
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DNAzyme-based biosensors and nanodevices
Chemical Communications, 2015DNAzymes, screened throughin vitroselection, have shown great promise as molecular tools in the design of biosensors and nanodevices.
Liang, Gong +7 more
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Catalytic DNAzymes: derivations and functions
Expert Opinion on Biological Therapy, 2008Although catalytic RNA enzymes (CRzs) are naturally occurring in many organisms, their DNA counterparts (CDzs) were developed by in vitro selection/evolution from random sequence libraries.To provide a brief overview of how CDzs have been selected in vitro, and of their properties and functions, as well as their possible future utility.We concentrated ...
Weihua, Pan, Gary A, Clawson
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