Results 1 to 10 of about 1,575 (172)

Constructing an Efficient Bacillus subtilis Spore Display by Using Cohesin−Dockerin Interactions [PDF]

Molecules, 2021
Bacillus subtilis spore display has become a field of increasing interest in the past two decades. To improve the efficiency of B. subtilis spore display, its directed modification was performed based on the cellulosome architecture by introducing onto ...
He Wang, Xiaomin Jiang, Yongchang Qian, Lianghong Yin   +3 more
doaj   +4 more sources

Dissection of an Anaerobic Fungal Cellulosomal Endoglucanase: Impact of the Dockerin Module on Activity, Thermostability, and Flexibility [PDF]

ACS Omega
Cellulosomal cellulases possess an extra noncatalytic module denominated dockerin, which interacts with the scaffolding via cohesion modules to organize the enzymes within the cellulosome.
Viviane Brito Andrade, Robson Tramontina, Dnane Vieira Almeida, Geizecler Tomazetto, Viviam M. da Silva, Frank Gabel, Yolanda M.B. Marcello, Ana Ligia Scott, Fabio Marcio Squina, Wanius Garcia   +9 more
doaj   +3 more sources

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes [PDF]

Scientific Reports, 2017
Abtract Cellulosomes are sophisticated multi-enzymatic nanomachines produced by anaerobes to effectively deconstruct plant structural carbohydrates.
Pedro Bule, Victor D. Alves, Vered Israeli-Ruimy, Ana L. Carvalho, Luís M. A. Ferreira, Steven P. Smith, Harry J. Gilbert, Shabir Najmudin, Edward A. Bayer, Carlos M. G. A. Fontes   +9 more
doaj   +7 more sources

Structural and functional insights into cellulosomes: masters of plant cell wall degradation [PDF]

Frontiers in Microbiology
Cellulosomes are complex multi-enzyme systems that enable efficient cellulose breakdown in some anaerobic bacteria and fungi. Understanding cellulosome functionality plays a crucial role in expanding their potential for industrial plant biomass ...
Nataša Lindič, Maša Vodovnik
doaj   +2 more sources

Study on the construction technology of β-alanine synthesizing Escherichia coli based on cellulosome assembly [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
Introduction: β-Alanine is the only β-amino acid in nature; it is widely used in food additives, medicines, health products, and surfactants. To avoid pollution caused by traditional production methods, the synthesis of β-alanine has been gradually ...
Jie Lu, Jie Lu, Guodong Wang, Guodong Wang, Cuiping Yang, Cuiping Yang, Zehao Peng, Zehao Peng, Lu Yang, Lu Yang, Bowen Du, Bowen Du, Chuanzhuang Guo, Songsen Sui, Jianbin Wang, Junlin Li, Ruiming Wang, Ruiming Wang, Junqing Wang, Junqing Wang   +19 more
doaj   +2 more sources

Improving the Synthesis Efficiency of Amino Acids Such as L-Lysine by Assembling Artificial Cellulosome Elements Dockerin Protein In Vivo

Fermentation, 2022
Cellulosome is a highly efficient multi-enzyme self-assembly system and is found on the extracellular surface or in the free environment of microorganisms. However, with a lack of Ca2+ in vivo, cellulosome assembly is challenging.
Nan Li, Le Xue, Zirui Wang, Peng Du, Piwu Li, Jing Su, Jing Xiao, Min Wang, Junqing Wang, Ruiming Wang   +9 more
doaj   +2 more sources

Elaborate cellulosome architecture of Acetivibrio cellulolyticus revealed by selective screening of cohesin–dockerin interactions [PDF]

PeerJ, 2014
Cellulosic waste represents a significant and underutilized carbon source for the biofuel industry. Owing to the recalcitrance of crystalline cellulose to enzymatic degradation, it is necessary to design economical methods of liberating the fermentable ...
Yuval Hamberg, Vered Ruimy-Israeli, Bareket Dassa, Yoav Barak, Raphael Lamed, Kate Cameron, Carlos M.G.A. Fontes, Edward A. Bayer, Daniel B. Fried   +8 more
doaj   +3 more sources

A genomic analysis reveals the diversity of cellulosome displaying bacteria [PDF]

Frontiers in Microbiology
IntroductionSeveral species of cellulolytic bacteria display cellulosomes, massive multi-cellulase containing complexes that degrade lignocellulosic plant biomass (LCB). A greater understanding of cellulosome structure and enzyme content could facilitate
Christine M. Minor, Christine M. Minor, Allen Takayesu, Allen Takayesu, Sung Min Ha, Lukasz Salwinski, Michael R. Sawaya, Matteo Pellegrini, Robert T. Clubb, Robert T. Clubb, Robert T. Clubb   +10 more
doaj   +2 more sources

In vitro assembly of the trehalose bi-enzyme complex with artificial scaffold protein [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
Introduction: Trehalose is a significant rare sugar known for its stable properties and ability to protect biomolecules from environmental factors.Methods: In this study, we present a novel approach utilizing a scaffold protein-mediated assembly method ...
Xiangyi Wang, Xiangyi Wang, Yi Jiang, Yi Jiang, Hongling Liu, Hongling Liu, Xinyi Zhang, Xinyi Zhang, Haibo Yuan, Haibo Yuan, Di Huang, Di Huang, Tengfei Wang, Tengfei Wang   +13 more
doaj   +2 more sources

A cellulosomal double-dockerin module from Clostridium thermocellum shows distinct structural and cohesin-binding features. [PDF]

Protein Sci
Cellulosomes are intricate cellulose‐degrading multi‐enzymatic complexes produced by anaerobic bacteria, which are valuable for bioenergy development and biotechnology.
Chen C, Yang H, Dong S, You C, Moraïs S, Bayer EA, Liu YJ, Xuan J, Cui Q, Mizrahi I, Feng Y.   +10 more
europepmc   +2 more sources