Designer Glycolysomes: Colocalisation of Glycolytic Enzymes on a Cellulosome‐Based Synthetic Protein Scaffold [PDF]
Microbial BiotechnologyIn systems biocatalysis, combining pathway enzymes in vitro allows for the conversion of basic substrates into more complex, valuable chemicals. However, in vitro enzyme cascades are not yet economically viable for large‐scale bio‐based chemical ...
Marte Elias +4 more
doaj +3 more sources
Tripartite binding mode of cohesin-dockerin complexes from Ruminococcus flavefaciens involving naturally truncated dockerins. [PDF]
J Biol ChemPolysaccharides in plant cell walls serve as a rich carbon and energy source, yet their structural complexity presents a barrier to efficient degradation. To address this, anaerobic microorganisms like R. flavefaciens have developed sophisticated multi-enzyme complexes known as cellulosomes, which enable the efficient breakdown of these recalcitrant ...
Duarte M +9 more
europepmc +5 more sources
Dual binding in cohesin-dockerin complexes: the energy landscape and the role of short, terminal segments of the dockerin module [PDF]
Scientific Reports, 2018 The assembly of the polysaccharide degradating cellulosome machinery is mediated by tight binding between cohesin and dockerin domains. We have used an empirical model known as FoldX as well as molecular mechanics methods to determine the free energy of ...
M. Wojciechowski +5 more
semanticscholar +3 more sources
Scientific Reports, 2018 Cellulosomes are highly sophisticated molecular nanomachines that participate in the deconstruction of complex polysaccharides, notably cellulose and hemicellulose.
Pedro Bule +11 more
doaj +3 more sources
eLife, 2015 Receptor-ligand pairs are ordinarily thought to interact through a lock and key mechanism, where a unique molecular conformation is formed upon binding.
Markus A Jobst +7 more
doaj +2 more sources
HLA-DM co-expression enhances MHC class II function in the magnetosome display system [PDF]
Microbiology SpectrumEfficient identification of antigenic peptides that bind to major histocompatibility complex class II (MHC II) molecules is crucial for vaccine and therapeutic development.
Ryoto Tomoe +3 more
doaj +2 more sources
Mapping the deformability of natural and designed cellulosomes in solution [PDF]
Biotechnology for Biofuels and Bioproducts, 2022 Background Natural cellulosome multi-enzyme complexes, their components, and engineered ‘designer cellulosomes’ (DCs) promise an efficient means of breaking down cellulosic substrates into valuable biofuel products.
Jonathan Dorival +11 more
doaj +2 more sources
Cohesin-dockerin code in cellulosomal dual binding modes and its allosteric regulation by proline isomerization [PDF]
Structure, 2019 Cellulose is the most abundant organic molecule on Earth and represents a renewable and practically everlasting feedstock for the production of biofuels and chemicals.
A. M. Vera +7 more
semanticscholar +5 more sources
Novel Clostridium thermocellum Type I Cohesin-Dockerin Complexes Reveal a Single Binding Mode* [PDF]
Journal of Biological Chemistry, 2012 Background: In general, dockerins present two homologous cohesin-binding interfaces, which confer increased flexibility into cellulosomes. Results: The structure of two novel Coh-Doc complexes reveals a dockerin single-binding mode.
J. Brás +9 more
semanticscholar +4 more sources
Atypical Cohesin-Dockerin Complex Responsible for Cell Surface Attachment of Cellulosomal Components [PDF]
Journal of Biological Chemistry, 2013 Background: The type IIIe CohE-XDoc interaction connects cellulosomal components to the cell wall. Results: The dockerin structure in the CohE-XDoc complex exhibits an atypical calcium-binding loop disrupted by a 13-residue insert.
O. Salama-Alber +8 more
semanticscholar +2 more sources