Results 21 to 30 of about 1,575 (172)

Analysis of cohesin-dockerin interactions using mutant dockerin proteins. [PDF]

FEMS microbiology letters, 2011
Clostridial cellulosomes are cellulolytic complexes that are formed by highly specific interactions between one of the repeated cohesin modules present in the scaffolding protein and a dockerin module of the catalytic components. Although Clostridium thermocellum Xyn11A dockerin has a typical C.
Kazutaka, Sakka, Yuka, Sugihara, Sadanari, Jindou, Makiko, Sakka, Minoru, Inagaki, Kazuo, Sakka, Tetsuya, Kimura   +6 more
openaire   +2 more sources

Cohesin-Dockerin Interaction in Cellulosome Assembly [PDF]

Journal of Biological Chemistry, 2001
The assembly of enzyme components into the cellulosome complex is dictated by the cohesin-dockerin interaction. In a recent article (Mechaly, A., Yaron, S., Lamed, R., Fierobe, H.-P., Belaich, A., Belaich, J.-P., Shoham, Y., and Bayer, E. A. (2000) Proteins 39, 170-177), we provided experimental evidence that four previously predicted dockerin residues
Adva Mechaly, Henri-Pierre Fierobe, Anne Belaich, Jean-Pierre Belaich, Raphael Lamed, Yuval Shoham, Edward A. Bayer   +6 more
openaire   +4 more sources

A dual cohesin–dockerin complex binding mode in Bacteroides cellulosolvens contributes to the size and complexity of its cellulosome

Journal of Biological Chemistry, 2021
The Cellulosome is an intricate macromolecular protein complex that centralizes the cellulolytic efforts of many anaerobic microorganisms through the promotion of enzyme synergy and protein stability.
Marlene Duarte, A. Viegas, V. Alves, J. Prates, L. Ferreira, S. Najmudin, E. Cabrita, A. Carvalho, C. Fontes, P. Bule   +9 more
semanticscholar   +1 more source

Direct Comparison of Lysine versus Site‐Specific Protein Surface Immobilization in Single‐Molecule Mechanical Assays**

Angewandte Chemie, Volume 135, Issue 32, August 7, 2023., 2023
The surface immobilization technique for atomic force microscopy/single‐molecule force spectroscopy is crucial for experimental yield, accuracy and resolution. Reported herein is that a commonly used lysine‐based immobilization can result in significant signal deterioration, including lowering of rupture forces, effective shortening of linkers, and ...
Haipei Liu, Zhaowei Liu, Mariana Sá Santos, Michael A. Nash   +3 more
wiley   +2 more sources

Development of a Novel Cell Surface Attachment System to Display Multi-Protein Complex Using the Cohesin-Dockerin Binding Pair

Journal of Microbiology and Biotechnology, 2021
Autodisplay of a multimeric protein complex on a cell surface is limited by intrinsic factors such as the types and orientations of anchor modules. Moreover, improper folding of proteins to be displayed often hinders functional cell surface display ...
Hyeok-jin Ko, Heesang Song, I. Choi
semanticscholar   +1 more source

Affinity-induced covalent protein-protein ligation via the SpyCatcher-SpyTag interaction

Green Carbon, 2023
Production of economically viable bioethanol is potentially an environmentally and financially worthwhile endeavor. One major source for fermentable sugars is lignocellulose. However, lignocellulosic biomass is difficult to degrade, owing to its inherent
Jacob O. Fierer, Omar E. Tovar-Herrera, Jonathan Y. Weinstein, Amaranta Kahn, Sarah Moraïs, Itzhak Mizrahi, Edward A. Bayer   +6 more
doaj   +1 more source

Designing chimeric enzymes inspired by fungal cellulosomes

Synthetic and Systems Biotechnology, 2020
Cellulosomes are synthesized by anaerobic bacteria and fungi to degrade lignocellulose via synergistic action of multiple enzymes fused to a protein scaffold.
Sean P. Gilmore, Stephen P. Lillington, Charles H. Haitjema, Randall de Groot, Michelle A. O'Malley   +4 more
doaj   +1 more source

Effects of dockerin domains onNeocallimastix frontalisxylanases [PDF]

FEMS Microbiology Letters, 2005
Two xylanase genes were cloned from the anaerobic fungus Neocallimastix frontalis. Xyn11A had a modular structure of two catalytic domains and two dockerin domains, while Xyn11B had one catalytic domain and two dockerin domains. The characteristics of the xylanases with and without dockerin domains were investigated.
Ya-Hui, Huang, Ching-Tsan, Huang, Ruey-Shyang, Hseu   +2 more
openaire   +2 more sources

Cohesin-dockerin interactions within and between Clostridium josui and Clostridium thermocellum: binding selectivity between cognate dockerin and cohesin domains and species specificity. [PDF]

The Journal of biological chemistry, 2004
The cellulosome components are assembled into the cellulosome complex by the interaction between one of the repeated cohesin domains of a scaffolding protein and the dockerin domain of an enzyme component. We prepared five recombinant cohesin polypeptides of the Clostridium thermocellum scaffolding protein CipA, two dockerin polypeptides of C ...
Sadanari, Jindou, Akane, Soda, Shuichi, Karita, Tsutomu, Kajino, Pierre, Béguin, J H David, Wu, Minoru, Inagaki, Tetsuya, Kimura, Kazuo, Sakka, Kunio, Ohmiya   +9 more
openaire   +2 more sources

Protein Engineering and High‐Throughput Screening by Yeast Surface Display: Survey of Current Methods

Small Science, Volume 3, Issue 12, December 2023., 2023
Presenting a focused review on novel methods for protein engineering, this article delves into cutting‐edge advancements in the field of yeast surface display. Central themes include titratable avidity, on‐yeast serology, unconventional antigens including cell lysates and membrane proteins, expanded chemistry, noncanonical amino acids, and continuous ...
Joanan Lopez-Morales, Rosario Vanella, Elizabeth A. Appelt, Sarah Whillock, Alexandra M. Paulk, Eric V. Shusta, Benjamin J. Hackel, Chang C. Liu, Michael A. Nash   +8 more
wiley   +1 more source