Results 31 to 40 of about 1,575 (172)

Cohesin-Dockerin Interactions of Cellulosomal Subunits of Clostridium cellulovorans [PDF]

open access: yesJournal of Bacteriology, 2001
ABSTRACT The cellulosome of Clostridium cellulovorans consists of three major subunits: CbpA, EngE, and ExgS. The C. cellulovorans scaffolding protein (CbpA) contains nine hydrophobic repeated domains (cohesins) for the binding of enzymatic subunits.
J S, Park, Y, Matano, R H, Doi
openaire   +2 more sources

The secretome of Thermococcus barophilus in the presence of carbohydrates and the potential role of the TrmBL4 regulator

open access: yesEnvironmental Microbiology Reports, Volume 15, Issue 6, Page 530-544, December 2023., 2023
The secretome of Thermococcus barophilus was explored in the light of sugar metabolism (maltose, maltodextrin and pectin). The expression of certain secreted proteins is directly dependent on the sugar regulator TrmBL4, as is the ABC maltose/maltodextrin transporter (MD system), but not the trehalose/maltose transport system (TM system).
Maria Batour   +5 more
wiley   +1 more source

Cellulosomics, a gene-centric approach to investigating the intraspecific diversity and adaptation of Ruminococcus flavefaciens within the rumen. [PDF]

open access: yesPLoS ONE, 2011
BackgroundThe bovine rumen maintains a diverse microbial community that serves to break down indigestible plant substrates. However, those bacteria specifically adapted to degrade cellulose, the major structural component of plant biomass, represent a ...
Jennifer M Brulc   +13 more
doaj   +1 more source

Unraveling enzyme discrimination during cellulosome assembly independent of cohesin–dockerin affinity [PDF]

open access: yesThe FEBS Journal, 2013
Bacterial cellulosomes are generally believed to assemble at random, like those produced by Clostridium cellulolyticum. They are composed of one scaffolding protein bearing eight homologous type I cohesins that bind to any of the type I dockerins borne by the 62 cellulosomal subunits, thus generating highly heterogeneous complexes. In the present study,
Romain Borne   +4 more
semanticscholar   +3 more sources

Co‑cultivation of the anaerobic fungus Caecomyces churrovis with Methanobacterium bryantii enhances transcription of carbohydrate binding modules, dockerins, and pyruvate formate lyases on specific substrates

open access: yesBiotechnology for Biofuels, 2021
Anaerobic fungi and methanogenic archaea are two classes of microorganisms found in the rumen microbiome that metabolically interact during lignocellulose breakdown.
Jennifer L. Brown   +15 more
doaj   +1 more source

Discovery of a bifunctional xylanolytic enzyme with arabinoxylan arabinofuranohydrolase‐d3 and endo‐xylanase activities and its application in the hydrolysis of cereal arabinoxylans

open access: yesMicrobial Biotechnology, Volume 16, Issue 7, Page 1536-1547, July 2023., 2023
1. A novel bifunctional xylanolytic enzyme (rAbf43A) was obtained.2. rAbf43A exhibits arabinoxylan arabinofuranohydrolase‐d3 and endo‐xylanase activities.3. rAbf43A shows obvious synergy with cognate bifunctional xylanase/feruloyl esterase. Abstract Xylanolytic enzymes, with both endo‐xylanase and arabinoxylan arabinofuranohydrolase (AXH) activities ...
Ruonan Wang   +4 more
wiley   +1 more source

The Cellulosome Paradigm in An Extreme Alkaline Environment

open access: yesMicroorganisms, 2019
Rapid decomposition of plant biomass in soda lakes is associated with microbial activity of anaerobic cellulose-degrading communities. The alkaliphilic bacterium, Clostridium alkalicellulosi, is the single known isolate from a soda lake that demonstrates
Paripok Phitsuwan   +4 more
doaj   +1 more source

Synthetic Multienzyme Assemblies for Natural Product Biosynthesis

open access: yesChemBioChem, Volume 24, Issue 6, March 14, 2023., 2023
Constructing multienzyme complexes has become an effective way to optimize metabolic flux and increase the titer of natural products in metabolic engineering recently. In this review, we summarize strategies for constructing synthetic multienzyme complexes in microbial cells and their applications in promoting natural product biosynthesis.
Min Liu   +4 more
wiley   +1 more source

Minimalistic Cellulosome of the Butanologenic Bacterium Clostridium saccharoperbutylacetonicum

open access: yesmBio, 2020
Clostridium saccharoperbutylacetonicum is a mesophilic, anaerobic, butanol-producing bacterium, originally isolated from soil. It was recently reported that C. saccharoperbutylacetonicum possesses multiple cellulosomal elements and would potentially form
Bosmat Levi Hevroni   +4 more
doaj   +1 more source

The need for an integrated multi‐OMICs approach in microbiome science in the food system

open access: yesComprehensive Reviews in Food Science and Food Safety, Volume 22, Issue 2, Page 1082-1103, March 2023., 2023
Abstract Microbiome science as an interdisciplinary research field has evolved rapidly over the past two decades, becoming a popular topic not only in the scientific community and among the general public, but also in the food industry due to the growing demand for microbiome‐based technologies that provide added‐value solutions.
Ilario Ferrocino   +15 more
wiley   +1 more source

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