Results 51 to 60 of about 1,575 (172)

Molecular mechanics studies of factors affecting overall rate in cascade reactions: Multi-enzyme colocalization and environment. [PDF]

Protein Sci
Abstract Millions of years of evolution have optimized many biosynthetic pathways by use of multi‐step catalysis. In addition, multi‐step metabolic pathways are commonly found in and on membrane‐bound organelles in eukaryotic biochemistry. The fundamental mechanisms that facilitate these reaction processes provide strategies to bioengineer metabolic ...
Kaushik S, Hung TI, Chang CA.
europepmc   +2 more sources

Cellulosome assembly revealed by the crystal structure of the cohesin–dockerin complex [PDF]

Proceedings of the National Academy of Sciences, 2003
The utilization of organized supramolecular assemblies to exploit the synergistic interactions afforded by close proximity, both for enzymatic synthesis and for the degradation of recalcitrant substrates, is an emerging theme in cellular biology.
Carvalho AL, Dias FMV, Prates JAM, Nagy T, Gilbert HJ, Davies GJ, Ferreira LMA, Romao MJ, Fontes CMGA   +8 more
openaire   +3 more sources

Effects of synthetic cohesin-containing scaffold protein architecture on binding dockerin-enzyme fusions on the surface of Lactococcus lactis

Microbial Cell Factories, 2012
Background The microbial synthesis of fuels, commodity chemicals, and bioactive compounds necessitates the assemblage of multiple enzyme activities to carry out sequential chemical reactions, often via substrate channeling by means of multi-domain or ...
Wieczorek Andrew S, Martin Vincent JJ
doaj   +1 more source

Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase

Microbial Cell Factories, 2013
Background Cellulosic biomass is considered as a promising alternative to fossil fuels, but its recalcitrant nature and high cost of cellulase are the major obstacles to utilize this material.
Kim Sujin, Baek Seung-Ho, Lee Kyusung, Hahn Ji-Sook   +3 more
doaj   +1 more source

Adaptor Scaffoldins: An Original Strategy for Extended Designer Cellulosomes, Inspired from Nature

mBio, 2016
Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled incorporation of recombinant dockerin-containing enzymes. The largest designer cellulosome reported to date is a chimeric scaffoldin that contains 6 cohesins.
Johanna Stern, Sarah Moraïs, Raphael Lamed, Edward A. Bayer   +3 more
doaj   +1 more source

Novel Organization and Divergent Dockerin Specificities in the Cellulosome System of Ruminococcus flavefaciens [PDF]

Journal of Bacteriology, 2003
ABSTRACT The DNA sequence coding for putative cellulosomal scaffolding protein ScaA from the rumen cellulolytic anaerobe Ruminococcus flavefaciens 17 was completed. The mature protein exhibits a calculated molecular mass of 90,198 Da and comprises three cohesin domains, a C-terminal dockerin, and a unique ...
Rincon M.T.1, Ding S.Y.2, McCrae S.I.1, Martin J.C.1, Aurilia V.3, Lamed, R.4, Shoham, Y.5, Bayer E.A.2, Flint H.J.1   +10 more
openaire   +2 more sources

Shedding Light on Microbial “Dark Matter”: Insights Into Novel Cloacimonadota and Omnitrophota From an Antarctic Lake

Frontiers in Microbiology, 2021
The potential metabolism and ecological roles of many microbial taxa remain unknown because insufficient genomic data are available to assess their functional potential.
Timothy J. Williams, Michelle A. Allen, Jonathan F. Berengut, Ricardo Cavicchioli   +3 more
doaj   +1 more source

Involvement of Both Dockerin Subdomains in Assembly of the Clostridium thermocellum Cellulosome [PDF]

Journal of Bacteriology, 1998
ABSTRACT Clostridium thermocellum produces an extracellular cellulase complex termed the cellulosome. It consists of a scaffolding protein, CipA, containing nine cohesin domains and a cellulose-binding domain, and at least 14 different enzymatic subunits, each containing a conserved duplicated sequence, or dockerin ...
B, Lytle, J H, Wu
openaire   +2 more sources

CRISPR-PIN: Modifying gene position in the nucleus via dCas9-mediated tethering

Synthetic and Systems Biotechnology, 2019
Spatial organization of DNA within the nucleus is important for controlling DNA replication and repair, genetic recombination, and gene expression. Here, we present CRISPR-PIN, a CRISPR/dCas9-based tool that allows control of gene Position in the Nucleus
Jyun-Liang Lin, Holly Ekas, Matthew Deaner, Hal S. Alper   +3 more
doaj   +1 more source

Environmental and Ecological Monitoring with Biodegradable Technologies

Advanced Science, Volume 13, Issue 4, 19 January 2026.
This review examines the development and application of wireless biodegradable sensors for environmental monitoring. It explores (bio)degradable materials, their degradation mechanisms in various environments, and non‐toxic fabrication techniques. Additionally, it addresses scalable production and sustainable powering solutions, emphasizing the high ...
Mohammad Javad Bathaei, Yaren Bathaei, Zhengwei Liao, Maryam Yazdanmehr, Sarab S. Sethi, Denys Nikolayev, Filipe Arroyo Cardoso, Clementine M. Boutry   +7 more
wiley   +1 more source