Results 141 to 150 of about 98,431 (180)
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Aptamers as the powerhouse of dot blot assays
Talanta, 2021Dot blot assays have always been associated with antibodies as the main molecular recognition element, which are widely employed in a myriad of diagnostic applications. With the rising of aptamers as the equivalent molecular recognition elements of antibodies, dot blot assays are also one of the diagnostic avenues that should be scrutinized for their ...
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2003
Nucleic acid hybridization is a very potent technique that can be used for the identification of DNA and RNA species with varying degree of homology and for the estimation of relative amounts of nucleic acid with known homolgy. In most cases, single-stranded (ss) (denatured) DNA or RNA is bound to a filter support (e.g., nitrocellulose) and incubated ...
H E, Bergmans, W, Gaastra
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Nucleic acid hybridization is a very potent technique that can be used for the identification of DNA and RNA species with varying degree of homology and for the estimation of relative amounts of nucleic acid with known homolgy. In most cases, single-stranded (ss) (denatured) DNA or RNA is bound to a filter support (e.g., nitrocellulose) and incubated ...
H E, Bergmans, W, Gaastra
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Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots
2015We demonstrate ultrasensitive fluorescence imaging of proteins on dot blots and western blots using a bright, compact, and orange-emitting semiconducting polymer dot (CNPPV). We achieved a detection limit at the single-picogram level in dot blots; with conventional western blot, we detected 50 pg of transferrin and trypsin inhibitor after SDS-PAGE and ...
Fangmao, Ye +2 more
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Automatic Analysis of Dot Blot Images
Intelligent Automation & Soft Computing, 2015This paper presents a method for the automatic analysis of macroarray (dot blot) images. The system developed receives as input a dot blot image, corrects it for grid rotation, identifies the visible markers and provides an evaluation of the status of each marker (ON/OFF).
Cristina M. R. Caridade +4 more
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1987
The techniques of Southern and Northern blotting have become widely known and are extensively used in the study of molecular genetics. Briefly, DNA is fragmented by incubation with restriction endonucleases and the fragments are then separated by electrophoresis.
Paul D. van Helden, C. Louise Olliver
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The techniques of Southern and Northern blotting have become widely known and are extensively used in the study of molecular genetics. Briefly, DNA is fragmented by incubation with restriction endonucleases and the fragments are then separated by electrophoresis.
Paul D. van Helden, C. Louise Olliver
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A dot blot assay for sulfated glycosaminoglycans
Journal of Biochemical and Biophysical Methods, 1989A dot blot assay for detection of low amounts of heparin and sulfated glycosaminoglycans (GAGs) is described. The detection range is between 25 ng/ml and 1000 ng/ml of heparin. The assay is based on the interference of sulfated GAGs with the binding of a synthetic ligand (described in this paper) to defined receptors like collagen type V and histones ...
J, Scheerer +3 more
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Dot blot assay for the serotyping of pneumococci
Journal of Clinical Microbiology, 1997To simplify the serotyping of Streptococcus pneumoniae, a dot bot assay has been developed and compared with the standard quellung reaction in 1,082 isolates. The technique has been demonstrated to be sensitive, specific, easy to perform, and inexpensive. The dot blot assay could be useful when large numbers of pneumococci have to be studied.
A, Fenoll, I, Jado, D, Vicioso, J, Casal
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The Dot Blot Direct Antiglobulin Test
American Journal of Clinical Pathology, 1987The direct antiglobulin test (DAT) is the most widely used serologic method to determine whether antibody or complement has bound to red blood cells in vivo. A solid phase DAT, based on the dot immunobinding technic, has been developed (DOT DAT). The solid phase was prepared by attaching anti-human IgG to nitrocellulose membranes.
F V, Plapp, J M, Rachel, L T, Sinor
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Detection of soya proteins in heated meat products by ?blotting? and ?dot blot?
Zeitschrift f�r Lebensmittel-Untersuchung und -Forschung, 1986Soy proteins (isolates, concentrates and texturates) as well as meat products containing soya isolate were analysed by SDS-electrophoresis. The separated proteins were blotted on nitrocellulose and stained with a selective immunoperoxidase system with the following sequence: primary (anti-soya) serum, goat anti-rabbit IgG serum and peroxidase ...
F W, Janssen, G, Voortman, J A, de Baaij
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