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Hybridoma Screening by Antibody Capture: Dot Blot
Cold Spring Harbor Protocols, 2021A dot blot is an appropriate hybridoma screening procedure when the antigen is a protein that is available in purified form. The antigen is bound directly to a nitrocellulose sheet and incubated with hybridoma tissue culture supernatant. A dot blot is widely used to determine the productivity of a given hybridoma, and this is described here. This assay
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Current Protocols in Molecular Biology, 1993
AbstractDot and slot blotting are simple techniques for immobilizing bulk unfractionated DNA on a nitrocellulose or nylon membrane. Hybridization analysis can then be carried out to determine the relative abundance of target sequences in the blotted DNA preparations. Dot and slot blots differ only in the geometry of the blot, a series of spots giving a
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AbstractDot and slot blotting are simple techniques for immobilizing bulk unfractionated DNA on a nitrocellulose or nylon membrane. Hybridization analysis can then be carried out to determine the relative abundance of target sequences in the blotted DNA preparations. Dot and slot blots differ only in the geometry of the blot, a series of spots giving a
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Origin and utility of the reverse dot–blot
Expert Review of Molecular Diagnostics, 2003Reverse allele specific oligonucleotide assays provide a robust method for the molecular characterization of high-mutation spectrum disorders. Commercial test have been developed for human leukocyte antigens class I and class II regions of human chromosome 6, the cystic fibrosis transmembrane conductance regulator at 7q31 and strains of human Hepatitis
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Blots, dots, amplification, and sequencing
2003Abstract Restriction enzymes (REs) are bacterial proteins that cut long, linear DNA molecules into smaller fragments. Restriction endonucleases are a major tool in recombinant DNA technology. A RE recognizes a specific sequence in DNA such as AGCT and cuts DNA wherever this combination of bases occurs in the genome.
John J O’Leary +2 more
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Dot blot assay of antibodies to Treponema pallidum
Doklady Biochemistry and Biophysics, 2014149 Among all infectious diseases, syphilis in recent years occupies one of the first places in terms of the number of cases per 100 thousand population [1]. One of the factors that negatively affect the epidemic situa tion is the insufficient sensitivity of screening methods of the diagnostics of early stages of the disease.
M B, Rayev +2 more
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Biologie in unserer Zeit, 2013
AbstractDie Übertragung von Nukleinsäuren oder Proteinen auf eine Trägermembran wird im Labor durch die Anfertigung eines “Blots” durchgeführt. Die Dotblot‐Technik kann hierbei als eine Art “Schnelltest” eingesetzt werden. DNA, RNA oder auch Proteinproben werden ohne vorherige Gelelektrophorese auf eine Membran übertragen und anschließend detektiert ...
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AbstractDie Übertragung von Nukleinsäuren oder Proteinen auf eine Trägermembran wird im Labor durch die Anfertigung eines “Blots” durchgeführt. Die Dotblot‐Technik kann hierbei als eine Art “Schnelltest” eingesetzt werden. DNA, RNA oder auch Proteinproben werden ohne vorherige Gelelektrophorese auf eine Membran übertragen und anschließend detektiert ...
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2011
This chapter provides the general workflow to simply entrap nucleic acids (in the specific case DNA) onto a Nylon membrane by dot-blot technique. The described method is a simple and fast procedure to transfer a known amount of sample onto an inherent support, such as a Nylon membrane.
Serena Bonin, Isabella Dotti
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This chapter provides the general workflow to simply entrap nucleic acids (in the specific case DNA) onto a Nylon membrane by dot-blot technique. The described method is a simple and fast procedure to transfer a known amount of sample onto an inherent support, such as a Nylon membrane.
Serena Bonin, Isabella Dotti
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Dot Blot Immunoassay for Detection of Human Semen
Journal of Immunoassay, 1992A sensitive and specific dot blot immunoassay based on prostate specific antigen (PSA) antibodies and a radiolabelled Protein A detection system was developed for detection of trace amounts of human semen in stains. The method was found highly sensitive and detected semen stain extracts diluted 10,000 times.
D V, Rao, V K, Kashyap
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A Computer Method for Scanning and Analysis of Dot Blots
DNA, 1985A system is described for computer analysis of autoradiograms produced from dot blots. The autoradiograms are first digitized on a rotating drum densitometer. The system automatically locates, integrates, and corrects the intensity of each spot for background.
R L, Williams +3 more
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A dot-blot assay for heparin-binding proteins
Analytical Biochemistry, 1986A method for the detection and quantitation of picomole amounts of heparin-binding proteins is described. Proteins are first spotted on nitrocellulose and then incubated with 125I-heparin. Binding of heparin to the proteins is detected by radioautography and quantitated by scanning densitometry; proteins are quantitated by densitometric analysis of the
N, Hirose +3 more
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