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Species with Haploid or Doubled Haploid Protocols

2021
In this chapter, we present a list of species (and few interspecific hybrids) where haploids and/or doubled haploids have been published, including the method by which they were obtained and the corresponding references. This list is an update of the compilation work of Maluszynski et al.
Jose M, Seguí-Simarro   +3 more
openaire   +2 more sources

Analysis of Ploidy in Haploids and Doubled Haploids

2021
Determination of the ploidy level is an essential step when trying to produce doubled haploids (DHs) in any species. Each species and method used to produce DHs has its own frequency of DH production, which means that the rest of plants produced stay haploid.
Ochatt, Sergio J, Seguí-Simarro, Jose
openaire   +3 more sources

Haploid and Doubled Haploid Technology

2007
The microspore culture technique has its wide applications in plant genetic research and breeding programmes in oilseed Brassicas due to its relative simplicity, efficiency in haploid and doubled haploid production, mutation and germplasm generation, and gene transformation.
Xu, L.   +6 more
openaire   +2 more sources

Production of Haploids and Doubled Haploids in Maize

2012
The in vivo haploid induction approach offers several advantages compared to the in vitro induction approach and recurrent self-pollination. It is currently the method of choice for inbred line development in many commercial maize breeding programs. Here, we describe the in vivo approach for generation of maternal doubled haploids (DHs).
Vanessa, Prigge, Albrecht E, Melchinger
openaire   +2 more sources

Doubled Haploid Technology: Generation of Doubled Haploid Maize Lines Using Haploid Inducers

Cold Spring Harbor Protocols
Doubled haploid (DH) technology allows for the development of completely homozygous lines from heterozygous plants in only two generations. This approach has been widely adopted in maize breeding programs, as it expedites the generation of inbred lines compared to traditional methods. The DH approach is based on the use of maize genotypes that have the
Vencke K. Grüning   +2 more
openaire   +2 more sources

Protocols for In Vivo Doubled Haploid (DH) Technology in Maize Breeding: From Haploid Inducer Development to Haploid Genome Doubling

2022
Doubled haploid (DH) technology reduces the time required to obtain homozygous genotypes and accelerates plant breeding among other advantages. It is established in major crop species such as wheat, barley, maize, and canola. DH lines can be produced by both in vitro and in vivo methods and the latter is focused here.
Siddique I. Aboobucker   +6 more
openaire   +3 more sources

Colchicine Alternatives for Chromosome Doubling in Maize Haploids for Doubled‐Haploid Production

Crop Science, 2016
In vivo production of doubled‐haploid (DH) lines in maize (Zea mays L.) routinely involves artificial chromosome doubling by colchicine treatment of seedlings. Because colchicine is a hazardous chemical, replacing it by less‐toxic alternatives would be highly desirable.
Albrecht E. Melchinger   +3 more
openaire   +1 more source

Doubled Haploids in Triticale

2015
Climate changes, a rapid pathogen evolution, and market change, put breeders under constant pressure to deliver new and better varieties in a shorter time while simultaneously reducing the costs of variety development. Substantial progress has been generated in triticale doubled haploid (DH) technologies during the last decade that widens their ...
Maria Wędzony   +5 more
openaire   +1 more source

HAPLOID PRODUCTION AND CHROMOSOME DOUBLING

Acta Horticulturae, 2006
In the applications of haploid plants, chromosome doubling has a major role and has been widely researched. Chromosome doubling restores fertility to plants and often results in genetically homozygous doubled haploids. In diploid and allopolyploid species these homozygous plants have the potential to become pure breeding new cultivars or the parents ...
K.J. Kasha   +3 more
openaire   +1 more source

Doubled haploids in breeding

2003
There are many factors influencing the deployment of doubled haploidy in breeding. Strategies for exploiting doubled haploids (DHs) vary within and among species and are dependent upon available technologies. Theoretical benefits and working protocols are not however sufficient to justify DH deployment in breeding.
W. T. B. Thomas   +2 more
openaire   +1 more source

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