Results 121 to 130 of about 664,193 (303)

Dynamic graph convolutional traffic flow prediction model based on attention and linear layer fusion

大数据
Accurate traffic volume prediction is crucial for optimizing the operational efficiency of road networks and alleviating urban traffic congestion. Traditional models rely on predefined static graph structures, making it difficult to capture dynamic ...
Wei Hui, Xiao Hongbo, Zou Beiji, Kui Xiaoyan, Xiao Jianhua, He Jiaju, He Niguli   +6 more
doaj  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Quantifying Spatiotemporal Heterogeneity of Tumor Metabolism and Vasculature with a Multiparametric Point-of-Investigation Microscope

BME Frontiers
Objective: The aim of this study was to develop and apply a dual-scale Capillary-Cell (CapCell) microscope to quantify spatial and temporal heterogeneity in tumor metabolism and vasculature during anti-angiogenic therapy.
Enakshi D. Sunassee, Marcia Cunha Dos Santos, Riley J. Deutsch-Williams, Sanjana Sankholkar, Megan Madonna, Gregory Palmer, Nirmala Ramanujam   +6 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Dual-pulse laser induced breakdown spectroscopy in the vacuum ultraviolet with ambient gas: spectroscopic analysis and optimization of limit of detection of carbon and sulfur in steel [PDF]

, 2012
Laser Induced Breakdown Spectroscopy (LIBS) in the vacuum ultraviolet (VUV) spectral region was applied to standard steel samples in dual-pulse excitation mode with an ambient gas. Two lasers were employed in collinear geometry, one as an ablation laser (
Jiang, Xi
core  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Viscous force and added mass for complex configuration with an implicit dual time method [PDF]

, 2007
This article obtains the viscous force and added mass for a complex configuration. its subject is a small AUV propelled by four pumps at stem, which is used to survey the ocean. The model in simulation is similar to actual body without any simplification
Wu LH(吴利红), Gong PL(公丕亮), Feng XS(封锡盛), Jin Z(金忠)   +3 more
core  

Semi-Dual Attention: A Structure-Preserving Attention Mechanism Derived from Semi-Dual Optimization Theory

ITEGAM-JETIA
Since the introduction of the Transformer architecture, attention mechanisms have become a core component of sequence modeling and large-scale pre-trained models. However, almost all existing mainstream attention methods are based on a "primal" modeling
Jincheng Zhang, Rukthin Laoha, Potsirin Limpinan, Thada Jantakoon   +3 more
doaj   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source