A Dynamic Clamp on Every Rig [PDF]
eneuro, 2017 AbstractThe dynamic clamp should be a standard part of every cellular electrophysiologist’s toolbox. That it is not, even 25 years after its introduction, comes down to three issues: money, the disruption that adding dynamic clamp to an existing electrophysiology rig entails, and the technical prowess required of experimenters.
Niraj S. Desai +2 more
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A dynamic clamp protocol to artificially modify cell capacitance [PDF]
eLife, 2022 Dynamics of excitable cells and networks depend on the membrane time constant, set by membrane resistance and capacitance. Whereas pharmacological and genetic manipulations of ionic conductances of excitable membranes are routine in electrophysiology ...
Paul Pfeiffer +5 more
doaj +3 more sources
High-speed dynamic-clamp interface
Journal of Neurophysiology, 2015 The dynamic-clamp technique is highly useful for mimicking synaptic or voltage-gated conductances. However, its use remains rare in part because there are few systems, and they can be expensive and difficult for less-experienced programmers to implement.
Yang, Y. +4 more
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Dynamic clamp with StdpC software [PDF]
Nature Protocols, 2011 Dynamic clamp is a powerful method that allows the introduction of artificial electrical components into target cells to simulate ionic conductances and synaptic inputs. This method is based on a fast cycle of measuring the membrane potential of a cell, calculating the current of a desired simulated component using an appropriate model and injecting ...
Kemenes, Ildikó +6 more
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A Hybrid Model for Safety Pharmacology on an Automated Patch Clamp Platform: Using Dynamic Clamp to Join iPSC-Derived Cardiomyocytes and Simulations of Ik1 Ion Channels in Real-Time [PDF]
Frontiers in Physiology, 2018 An important aspect of the Comprehensive In Vitro Proarrhythmia Assay (CiPA) proposal is the use of human stem cell-derived cardiomyocytes and the confirmation of their predictive power in drug safety assays.
Birgit Goversen +7 more
doaj +3 more sources
Injection of IK1 through dynamic clamp can make all the difference in patch-clamp studies on hiPSC-derived cardiomyocytes [PDF]
Frontiers in Physiology, 2023 Human-induced stem cell-derived cardiomyocytes (hiPSC-CMs) are a valuable tool for studying development, pharmacology, and (inherited) arrhythmias. Unfortunately, hiPSC-CMs are depolarized and spontaneously active, even the working cardiomyocyte subtypes
Arie O. Verkerk +2 more
doaj +2 more sources
Light-Activated Dynamic Clamp Using iPSC-Derived Cardiomyocytes [PDF]
Biophys J, 2018 iPSC-derived cardiomyocytes (iPSC-CMs) are a potentially advantageous platform for drug screening because they provide a renewable source of human cardiomyocytes.
Quach, Bonnie +3 more
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Dynamic Clamp in Cardiac and Neuronal Systems Using RTXI [PDF]
Methods Mol Biol, 2014 The injection of computer-simulated conductances through the dynamic clamp technique has allowed researchers to probe the intercellular and intracellular dynamics of cardiac and neuronal systems with great precision.
Christini, David J. +4 more
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The human dynamic clamp as a paradigm for social interaction [PDF]
Proceedings of the National Academy of Sciences, 2014 Significance The human dynamic clamp (HDC) is proposed as a general paradigm for studies of elementary forms of social behavior in complex biological systems. HDC enables parametric control of real-time bidirectional interaction between humans and empirically grounded theoretical models of coordination dynamics.
Guillaume, Dumas +3 more
openaire +3 more sources
Patch-Clamp Recording from Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Improving Action Potential Characteristics through Dynamic Clamp [PDF]
Int J Mol Sci, 2017 Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) hold great promise for studying inherited cardiac arrhythmias and developing drug therapies to treat such arrhythmias.
Christiaan Veerman +11 more
core +2 more sources