Results 201 to 210 of about 28,974 (239)

Rapid Purification of Native Dynamin I and Colorimetric GTPase Assay

2005
Dynamin I is a large GTPase enzyme required in membrane constriction and fission during multiple forms of endocytosis. The first method described here is for the rapid purification of native dynamin from peripheral membrane extracts of sheep brain using ammonium sulfate precipitation and affinity purification on recombinant SH3 domains.
Annie, Quan, Phillip J, Robinson
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Myristyl Trimethyl Ammonium Bromide and Octadecyl Trimethyl Ammonium Bromide Are Surface-Active Small Molecule Dynamin Inhibitors that Block Endocytosis Mediated by Dynamin I or Dynamin II

Molecular Pharmacology, 2007
Dynamin is a GTPase enzyme involved in membrane constriction and fission during endocytosis. Phospholipid binding via its pleckstrin homology domain maximally stimulates dynamin activity. We developed a series of surface-active small-molecule inhibitors, such as myristyl trimethyl ammonium bromide (MiTMAB) and octadecyltrimethyl ammonium bromide ...
Quan, Annie, McGeachie, Andrew B., Keating, Damien J., van Dam, Ellen M., Rusak, Jenny, Chau, Ngoc, Malladi, Chandra S., Chen, Chen, McCluskey, Adam, Cousin, Michael A., Robinson, Phillip J.   +10 more
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Resistance of Paramyxoviridae to Type I Interferon-Induced Bos taurus Mx1 Dynamin

Journal of Interferon & Cytokine Research, 2005
Typical targets of type I interferon (IFN)-induced antiviral Mx proteins known to date have been shown to share a common profile: single-stranded negative-sense RNA viruses. Among them, human MxA is known to interfere with the replication of measles, human, and bovine parainfluenza-3 viruses (BoPi3V), that is, three members of the Paramyxoviridae ...
Leroy, Michael, Baise, Etienne, Pire, Grégory, Gérardin, Joël, Desmecht, Daniel   +4 more
openaire   +3 more sources

Age‐ and experience‐dependent expression of Dynamin I and Synaptotagmin I in cat visual system

Journal of Comparative Neurology, 2007
AbstractDynamin I (Dyn I) and Synaptotagmin I (Syt I) are essential for endocytosis‐exocytosis processes, thus for neurotransmission. Despite their related function at presynaptic terminals, Dyn I and Syt I displayed opposite expression patterns during visual cortex maturation in the cat.
Lieselotte, Cnops, Tjing-Tjing, Hu, Jozef, Vanden Broeck, Kalina, Burnat, Gert, Van Den Bergh, Lutgarde, Arckens   +5 more
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Negative Regulation of YY1 Transcription Factor on the Dynamin I Gene Promoter

Biochemical and Biophysical Research Communications, 2001
Dynamin I is highly expressed in brain and plays a critical role in clathrin-mediated endocytosis and synaptic vesicle recycling. To elucidate the molecular mechanism by which expression of dynamin I is tissue-specifically regulated, we previously cloned and characterized the promoter of the mouse dynamin I gene and suggested that there is a negative ...
J, Yoo, M J, Jeong, S S, Lee, K I, Lee, B M, Kwon, Y M, Park, M Y, Han   +6 more
openaire   +2 more sources

Dynamin- and clathrin-dependent endocytic pathway in unicellular eukaryote Paramecium

Biochemistry and Cell Biology, 2004
The first evidence of dynamin presence and its colocalization with clathrin in the compartment involved in Paramecium receptor-mediated endocytosis is presented. We identified dynamin by cloning, Western blotting, and immunodetection in confocal and electron microscopy. The partial genes, which we have designated ParDyn1 and ParDyn2, are 1091 bp long,
Jolanta, Wiejak, Liliana, Surmacz, Elzbieta, Wyroba   +2 more
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Small Molecule Inhibitors of Dynamin I GTPase Activity:  Development of Dimeric Tyrphostins

Journal of Medicinal Chemistry, 2005
Dynamin I is a GTPase enzyme required for endocytosis and is an excellent target for the design of potential endocytosis inhibitors. Screening of a library of tyrphostins, in our laboratory, against the GTPase activity of dynamin I gave rise to a microM potent lead, 2-cyano-3-(3,4-dihydroxyphenyl)thioacrylamide (1, IC50 70 microM).
Hill, Timothy, Odell, Luke R., Edwards, Jennifer K., Graham, Mark E., McGeachie, Andrew B., Rusak, Jenny, Quan, Annie, Abagyan, Ruben, Scott, Janet L., Robinson, Phillip J., McCluskey, Adam   +10 more
openaire   +5 more sources

Calcineurin Inhibition of Dynamin I GTPase Activity Coupled to Nerve Terminal Depolarization

Science, 1994
Dynamin I is a nerve terminal phosphoprotein with intrinsic guanosine triphosphatase (GTPase) activity that is required for endocytosis. Upon depolarization and synaptic vesicle recycling, dynamin I undergoes a rapid dephosphorylation. Dynamin I was found to be a specific high-affinity substrate for calcineurin in vitro.
J P, Liu, A T, Sim, P J, Robinson
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Interaction of dynamin I with NAP-22, a neuronal protein enriched in the presynaptic region

Neuroscience Letters, 2018
Neurons have well-developed membrane microdomains called "rafts" that are recovered as a detergent-resistant low-density membrane microdomain fraction (DRM). NAP-22 is one of the major protein components of neuronal DRM and localizes in the presynaptic region.
Satoko, Ueno, Hiroshi, Miyoshi, Yoko, Maruyama, Mitsuhiro, Morita, Shohei, Maekawa   +4 more
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1,8-Naphthalimide derivatives: new leads against dynamin I GTPase activity

Organic & Biomolecular Chemistry, 2015
Fragment-based in silico screening against dynamin I (dynI) GTPase activity identified the 1,8-naphthalimide framework as a potential scaffold for the design of new inhibitors targeting the GTP binding pocket of dynI.
Abdel-Hamid, Mohammed K., Macgregor, Kylie A., Odell, Luke R., Chau, Ngoc, Mariana, Anna, Whiting, Ainslie, Robinson, Phillip J., McCluskey, Adam   +7 more
openaire   +2 more sources