Results 1 to 10 of about 119,730 (299)

A new method for isolation and purification of fusion-competent inhibitory synaptic vesicles [PDF]

Current Research in Physiology
Synaptic vesicles specific to inhibitory GABA-releasing neurons are critical for regulating neuronal excitability. To study the specific molecular composition, architecture, and function of inhibitory synaptic vesicles, we have developed a new method to ...
Nisha Gopal, Jeremy Leitz, Chuchu Wang, Luis Esquivies, Richard A. Pfuetzner, Axel T. Brunger   +5 more
doaj   +2 more sources

Recently recycled synaptic vesicles use multi-cytoskeletal transport and differential presynaptic capture probability to establish a retrograde net flux during ISVE in central neurons [PDF]

Frontiers in Cell and Developmental Biology, 2023
Presynapses locally recycle synaptic vesicles to efficiently communicate information. During use and recycling, proteins on the surface of synaptic vesicles break down and become less efficient.
Mason Parkes, Nathan L. Landers, Michael W. Gramlich   +2 more
doaj   +2 more sources

Brain-derived synaptic vesicles have an intrinsic ability to sequester tubulin [PDF]

BMC Biology
Background The presence and function of microtubules within the synaptic bouton has long been under investigation. In recent years, evidence has accumulated that connects the synaptic vesicle cluster to the local dynamics of microtubule ends. Nonetheless,
Tiago Mimoso, Aleksandr Korobeinikov, Alexander Stein, Dragomir Milovanovic, Silvio O. Rizzoli, Sarah Köster, Sofiia Reshetniak   +6 more
doaj   +2 more sources

Altered exocytosis of inhibitory synaptic vesicles at single presynaptic terminals of cultured striatal neurons in a knock-in mouse model of Huntington’s disease [PDF]

Frontiers in Molecular Neuroscience, 2023
Huntington’s disease (HD) is a progressive dominantly inherited neurodegenerative disease caused by the expansion of a cytosine-adenine-guanine (CAG) trinucleotide repeat in the huntingtin gene, which encodes the mutant huntingtin protein containing an ...
Chen Xu, Sidong Chen, Xingxiang Chen, Ka Hei Ho, Chungwon Park, Chungwon Park, Hanna Yoo, Suk-Ho Lee, Hyokeun Park, Hyokeun Park, Hyokeun Park   +10 more
doaj   +2 more sources

In vitro fusion of single synaptic and dense core vesicles reproduces key physiological properties

Nature Communications, 2019
Regulated exocytosis of neuronal synaptic vesicles is substantially faster than that of endocrine dense core vesicles despite similar molecular machineries.
Alex J. B. Kreutzberger, Volker Kiessling, Christopher Stroupe, Binyong Liang, Julia Preobraschenski, Marcelo Ganzella, Mark A. B. Kreutzberger, Robert Nakamoto, Reinhard Jahn, J. David Castle, Lukas K. Tamm   +10 more
doaj   +2 more sources

Tau forms synaptic nano-biomolecular condensates controlling the dynamic clustering of recycling synaptic vesicles [PDF]

Nature Communications, 2023
Neuronal communication relies on the release of neurotransmitters from various populations of synaptic vesicles. Despite displaying vastly different release probabilities and mobilities, the reserve and recycling pool of vesicles co-exist within a single
Shanley F. Longfield, Mahdie Mollazade, Tristan P. Wallis, Rachel S. Gormal, Merja Joensuu, Jesse R. Wark, Ashley J. van Waardenberg, Christopher Small, Mark E. Graham, Frédéric A. Meunier, Ramón Martínez-Mármol   +10 more
doaj   +2 more sources

Methods of measuring presynaptic function with fluorescence probes

Applied Microscopy, 2021
Synaptic vesicles, which are endogenous to neurotransmitters, are involved in exocytosis by active potentials and release neurotransmitters. Synaptic vesicles used in neurotransmitter release are reused via endocytosis to maintain a pool of synaptic ...
Yeseul Jang, Sung Rae Kim, Sung Hoon Lee
doaj   +1 more source

Real-time three-dimensional tracking of single vesicles reveals abnormal motion and pools of synaptic vesicles in neurons of Huntington's disease mice

iScience, 2021
Summary: Although defective synaptic transmission was suggested to play a role in neurodegenerative diseases, the dynamics and vesicle pools of synaptic vesicles during neurodegeneration remain elusive.
Sidong Chen, Hanna Yoo, Chun Hei Li, Chungwon Park, Gyunam Park, Li Yang Tan, Sangyong Jung, Hyokeun Park   +7 more
doaj   +1 more source

An analytical method to measure the contribution of clear synaptic and dense-core peri-synaptic vesicles to neurotransmitter release from synaptic terminals with two classes of secretory vesicles

MethodsX, 2021
Two types of secretory vesicles co-exist at some presynaptic terminals. Clear synaptic vesicles (CSV) release their contents at the synaptic active zone, upon single impulses, while dense-core vesicles (DCV) usually release their contents in the ...
Citlali Trueta
doaj   +1 more source

Ultrastructural and functional fate of recycled vesicles in hippocampal synapses [PDF]

, 2015
Efficient recycling of synaptic vesicles is thought to be critical for sustained information transfer at central terminals. However, the specific contribution that retrieved vesicles make to future transmission events remains unclear.
A Denker, A Denker, A Denker, A Ratnayaka, A Ratnayaka, AA Alabi, AW Henkel, AW Henkel, B Granseth, C Rosenmund, D Kamin, DM Ramirez, EA Lemke, FE Schweizer, G Miesenbock, H Park, J Raingo, JL Pyle, K Staras, KJ Darcy, KJ Darcy, M Bal, M Ertunc, M Frerking, MA Colicos, MA Gaffield, MW Fowler, N Harata, O Shupliakov, RP de Lange, S Sankaranarayanan, S Sankaranarayanan, S Tsuriel, S Watanabe, S Watanabe, SO Rizzoli, SO Rizzoli, SS Zakharenko, T Schikorski, T Schikorski, TA Ryan, TA Ryan, TC Sudhof, V Marra, V Marra, V Westphal, WJ Betz, Y Hua, Y Sara, Y Zhu, Z Hua, Z Li   +51 more
core   +5 more sources