Results 161 to 170 of about 131,570 (298)
Biotechnology and Bioengineering, EarlyView.SpyCatcher‐mi3 nanoparticles displaying RBD‐SD1 from MERS‐CoV, NL140422, and HKU4 elicited robust and cross‐reactive IgG responses in mice. Only MERS‐CoV RBD‐SD1 induced neutralizing antibodies against MERS‐CoV and protected human DPP4 mice from a MERS‐CoV challenge, indicating conserved serologic but limited cross‐neutralizing epitopes.
Peter J. Halfmann +9 more
wiley +1 more source
High-level extracellular production of an alkaline pectate lyase in E. coli BL21 (DE3) and its application in bioscouring of cotton fabric. [PDF]
3 Biotech, 2020 Zhen J +9 more
europepmc +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT Microbial production of 1,3‐butanediol (1,3‐BDO) offers a renewable route to this versatile C4 chemical. However, the low performance of CoA‐acylating butyraldehyde dehydrogenase (Bld), which contains a catalytic cysteine, limits efficient production in recombinant Escherichia coli (E. coli).
Seunghyun Cho +5 more
wiley +1 more source
Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli [PDF]
, 2017 Combination of an origin repair mutagenesis system with a new mutS host strain increased the efficiency of mutagenesis from 46% to 75% mutant clones. Overexpression with the T7 expression system afforded large quantities of proteins from mutant strains ...
Koebnik, Ralf +4 more
core
Genetically Programmed Control of Overflow Metabolism
Biotechnology and Bioengineering, EarlyView.ABSTRACT Overflow metabolism is a highly undesirable phenomenon that occurs in virtually all cell factories. Although several schemes have been applied to avoid overflow metabolism, this problem remains far from being solved. In this study, a genetic sensor for detecting overflow metabolism was designed, and its substrate specificity and reversibility ...
Lærke M. Jensen +3 more
wiley +1 more source
Engineering Murine Cross‐Reactivity Into an Affibody to Human Death Receptor 5
Biotechnology and Bioengineering, EarlyView.ABSTRACT Interspecies cross‐reactive protein therapeutics that target conserved epitopes across species are critical for translational research. The present study showcases the engineering of an affibody molecule, originally discovered for binding to human death receptor 5 (hDR5) with 94 nM affinity, to simultaneously acquire cross‐reactivity to murine
Tse‐Han Kuo +3 more
wiley +1 more source
Expression of chIL-6 gene encoding chicken interleukin-6 in E. coli BL21(DE3) [PDF]
TAP CHI SINH HOC, 2012 Vu Thi Thu Huyen +3 more
openaire +1 more source
Engineered B7‐H3 Binding in Modular Gp2 Miniproteins
Biotechnology and Bioengineering, EarlyView.ABSTRACT B7‐H3, an important immune checkpoint modulator of T‐cell function, is a tumor vasculature biomarker and is overexpressed in a variety of cancers. Its expression is associated with tumor growth, metastasis, and poor clinical prognosis, which makes B7‐H3 an appealing target for diagnostics and therapeutics.
Abbigael Harthorn +5 more
wiley +1 more source
Impact of Glycerol as Carbon Source onto Specific Sugar and Inducer Uptake Rates and Inclusion Body Productivity in E. coli BL21(DE3). [PDF]
Bioengineering (Basel), 2017 Kopp J +6 more
europepmc +1 more source
Cytoskeleton, EarlyView.ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas +5 more
wiley +1 more source