Results 171 to 180 of about 92,801 (255)

Benzoxaborole-Based Inhibitors Block LexA Autocleavage and Suppress SOS-Dependent Adaptive Phenotypes in <i>Escherichia coli</i>. [PDF]

open access: yesAntibiotics (Basel)
Bellio P   +6 more
europepmc   +1 more source

Production of potential subunit vaccine against swine erysipelas in fed-batch cultures of E. coli BL21(DE3)

open access: yesNew Biotechnology, 2009
A.J. Da Silva   +5 more
openaire   +1 more source

The tryptophan prenyltransferase ComQ from Bacillus subtilis 168 can prenylate daptomycin at Trp1

open access: yesThe FEBS Journal, EarlyView.
ComQ168 from Bacillus subtilis 168 catalyzes C‐terminal tryptophan farnesylation of ComX to generate a competence‐inducing pheromone. Recombinant ComQ168 also modifies cyclic peptides, including Daptomycin, likely at the N‐terminal tryptophan. Structural modeling highlights a C‐terminal binding region, supporting substrate promiscuity and establishing ...
Yanli Xu   +2 more
wiley   +1 more source

Cryo‐EM structure of GH43 β‐Xylosidase from Enterobacter cloacae provides insights into substrate specificity and the role of an auxiliary domain in enzymatic activity

open access: yesThe FEBS Journal, EarlyView.
Xylan, the second most abundant plant cell wall polysaccharide, is degraded by β‐xylanases and β‐xylosidases. Here, we present the 2.65 Å cryo‐EM structure of Enterobacter cloacae β‐xylosidase (EcXyl43, GH43) and the 2.4 Å X‐ray structure of its inactive F507A mutant.
Lorenzo Briganti   +8 more
wiley   +1 more source

Strategies for recombinant laccase expression and their roles in environmental remediation. [PDF]

open access: yesWorld J Microbiol Biotechnol
Bannach-Machado I   +5 more
europepmc   +1 more source

RNF13 regulates the endolysosomal pathway through interaction with the small GTPase Arl8B

open access: yesThe FEBS Journal, EarlyView.
The E3 ubiquitin ligase RNF13 functions as a regulatory scaffold, organizing Arl8B‐dependent complexes and tuning downstream signaling that controls endolysosomal organization and trafficking. In this model, wild‐type RNF13 associates with Arl8B on endolysosomes, whereas Arl8B (E22D/F55A) or RNF13 (L244A) variants weaken complex assembly, potentially ...
Audrey M. Sénécal   +4 more
wiley   +1 more source

The sorting nexin Snx21 promotes flotillin‐mediated endocytosis

open access: yesThe FEBS Journal, EarlyView.
In this study, we describe a novel function of the previously uncharacterized Snx21 protein as a specific positive regulator of flotillin‐mediated endocytic trafficking. Snx21 is recruited to endosomal membranes via binding to PI(3)P, and it colocalizes with flotillins on the surface of late endosomes.
Tamás Maruzs   +9 more
wiley   +1 more source

A katE katG double-knockout E. coli strain eliminates the risk of catalase contamination in recombinant proteins. [PDF]

open access: yesAppl Microbiol Biotechnol
Scholz AT   +12 more
europepmc   +1 more source

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