Benzoxaborole-Based Inhibitors Block LexA Autocleavage and Suppress SOS-Dependent Adaptive Phenotypes in <i>Escherichia coli</i>. [PDF]
Bellio P +6 more
europepmc +1 more source
The tryptophan prenyltransferase ComQ from Bacillus subtilis 168 can prenylate daptomycin at Trp1
ComQ168 from Bacillus subtilis 168 catalyzes C‐terminal tryptophan farnesylation of ComX to generate a competence‐inducing pheromone. Recombinant ComQ168 also modifies cyclic peptides, including Daptomycin, likely at the N‐terminal tryptophan. Structural modeling highlights a C‐terminal binding region, supporting substrate promiscuity and establishing ...
Yanli Xu +2 more
wiley +1 more source
Characterization of novel cellobiose 2-epimerases from Teredinibacter Haidensis and Cellvibrio Japonicus. [PDF]
Liu Y, Lutz-Wahl S, Fischer L.
europepmc +1 more source
Xylan, the second most abundant plant cell wall polysaccharide, is degraded by β‐xylanases and β‐xylosidases. Here, we present the 2.65 Å cryo‐EM structure of Enterobacter cloacae β‐xylosidase (EcXyl43, GH43) and the 2.4 Å X‐ray structure of its inactive F507A mutant.
Lorenzo Briganti +8 more
wiley +1 more source
Strategies for recombinant laccase expression and their roles in environmental remediation. [PDF]
Bannach-Machado I +5 more
europepmc +1 more source
RNF13 regulates the endolysosomal pathway through interaction with the small GTPase Arl8B
The E3 ubiquitin ligase RNF13 functions as a regulatory scaffold, organizing Arl8B‐dependent complexes and tuning downstream signaling that controls endolysosomal organization and trafficking. In this model, wild‐type RNF13 associates with Arl8B on endolysosomes, whereas Arl8B (E22D/F55A) or RNF13 (L244A) variants weaken complex assembly, potentially ...
Audrey M. Sénécal +4 more
wiley +1 more source
Developing an <i>E. coli</i> heterologous expression system for characterizing a marine debrominase from <i>Roseobacter</i> sp. [PDF]
Li X +7 more
europepmc +1 more source
The sorting nexin Snx21 promotes flotillin‐mediated endocytosis
In this study, we describe a novel function of the previously uncharacterized Snx21 protein as a specific positive regulator of flotillin‐mediated endocytic trafficking. Snx21 is recruited to endosomal membranes via binding to PI(3)P, and it colocalizes with flotillins on the surface of late endosomes.
Tamás Maruzs +9 more
wiley +1 more source
A katE katG double-knockout E. coli strain eliminates the risk of catalase contamination in recombinant proteins. [PDF]
Scholz AT +12 more
europepmc +1 more source

