Results 211 to 220 of about 92,801 (255)

The SPI-6 T6SS gene cluster from <i>Salmonella</i> Tennessee encodes a new antibacterial nuclease effector protein. [PDF]

Front Microbiol
Vargas-Del Río C, Urrea J, Parra-Calisto A, Blondel CJ, Avilés A, Salazar-Salas F, Espinoza-Jara P, Mora M, Rivera D, Amaya FA, Santiviago CA, Moreno-Switt A, Pezoa D.   +12 more
europepmc   +1 more source

Coordinating Calvin Cycle and Glycolysis in <i>Escherichia coli</i>. [PDF]

ACS Synth Biol
Lin YJ, Chen HT, Lin PY, Lai YJ, Li SY.
europepmc   +1 more source

RETRACTED: Advanced machine learning-guided optimization platform for high-yield soluble expression of Pseudomonas aeruginosa exotoxin A in engineered Escherichia coli strains.

PLoS One
Mohammad SF, Ali F, Shynara M.
europepmc   +1 more source

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A Guideline to Set Up Cascaded Continuous Cultivation with E. coli Bl21 (DE3)

2021
Continuous processing allows to maximize space-time yields and is implemented in many industrial branches. However, in manufacturing of value added compounds produced with microbial hosts, continuous processing is not state-of-the-art yet. This is because fluctuating productivity causes unwanted process deviations.
Julian, Kopp, Oliver, Spadiut
openaire   +2 more sources

On the expression of recombinant Cas9 protein in E. coli BL21(DE3) and BL21(DE3) Rosetta strains

Journal of Biotechnology, 2019
The CRISPR-Cas9 system is a new tool that has been extensively used for genome editing. The system is composed of a Cas9 endonuclease, which has the function of cleaving DNA at a specific site, and a guide RNA (gRNA), which contains the sequence of the cleavage site that is the target of editing.
Gabriela Pannunzio, Carmignotto, Adriano Rodrigues, Azzoni   +1 more
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Overexpression of a lethal methylase, M.TneDI, in E. coli BL21(DE3)

Biotechnology Letters, 2014
A pET-based vector pDH21 expressing the methylase, M.TneDI (recognizing CGCG) from Thermotoga was constructed, and transformed into E. coli BL21(DE3). Despite E. coli BL21(DE3) being McrBC positive, 30 transformants were isolated, which were suspected to be McrBC(-) mutants. The overexpression of M.TneDI was verified by SDS-PAGE analysis.
Hui, Xu, Dongmei, Han, Zhaohui, Xu
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Rapid label‐free quantitative analysis of the E. coli BL21(DE3) inner membrane proteome

PROTEOMICS, 2015
Biological membranes define cells and cellular compartments and are essential in regulating bidirectional flow of chemicals and signals. Characterizing their protein content therefore is required to determine their function, nevertheless, the comprehensive determination of membrane‐embedded sub‐proteomes remains challenging.
Malvina, Papanastasiou, Georgia, Orfanoudaki, Nikos, Kountourakis, Marina, Koukaki, Marios Frantzeskos, Sardis, Michalis, Aivaliotis, Konstantinos C, Tsolis, Spyridoula, Karamanou, Anastassios, Economou   +8 more
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CLONING AND EXPRESSION OFΒSUBUNIT GENE OF PHYCOCYANINFROM SPIRULINALAXA IN E. COLI BL21(DE3)

Anbar Science Journal
This study focuses on the cloning and expression of the β-subunit gene of C-phycocyanin (C-PC) from Spirulina laxa in Escherichia coli BL21 (DE3). C-phycocyanin, a significant light-harvesting protein found in cyanobacteria, is known for its pharmacological properties, including antioxidant and antifungal activities.
Noor K. Shaker, Harith K. Buniya
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Enhanced anticoagulant activity of hirudin-i analogue co-expressed with arylsulfotransferase in periplasm of E. coli BL21(DE3)

Journal of Biotechnology, 2020
Hirudin, a blood anticoagulant, is the most potent natural thrombin inhibitor of leech origin. Its application is limited because it is difficult to obtain abundant natural hirudin directly from the leech. Although some bioengineering methods can significantly increase the production of hirudin, the reduced efficacy of recombinant hirudin (rH) remains ...
Kamal, Rai, Xiaohui, Chu, Zixian, Bao, Yunlong, Liang, Xingang, Wang, Junqing, Yang, Mo, Xian, Yue, Sun, Rui, Nian   +8 more
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