Results 221 to 230 of about 131,570 (298)

S‐Adenosylmethionine (SAM) hydrolases counter increased SAM epimerisation in thermophilic archaea

The FEBS Journal, EarlyView.
S‐Adenosyl‐l‐methionine (SAM) is a vital enzyme cofactor. Epimerisation at the sulfonium centre of biologically active (SS,SCα)‐SAM is driven by heat, yielding biologically inactive (RS,SCα)‐SAM. Here, two novel archaeal SAM hydrolases from the thermophilic Sulfolobus acidocaldarius and the halophilic Haloferax volcanii are shown to cleave (RS,SCα)‐SAM.
Agnes Bartels, Michael K. F. Mohr, Phillip Nußbaum, Marie Joest, Bianca Wassmer, Laurent Rasquin, Sonja‐Verena Albers, Jennifer N. Andexer   +7 more
wiley   +1 more source

Efficient whole-cell biotransformation for gastrodin production via glycosyltransferase engineering and process optimization. [PDF]

Synth Syst Biotechnol
Wang X, Zhang J, Li T, Wang F, Wang Z, Li X.   +5 more
europepmc   +1 more source

Production of potential subunit vaccine against swine erysipelas in fed-batch cultures of E. coli BL21(DE3)

New Biotechnology, 2009
A.J. Da Silva, A.C. Horta, M. Iemma, M.T. Novo, T.C. Zangirolami, R. Giordano   +5 more
openaire   +1 more source

Discovery of a cryptic aminoacidic triad involved in the temperature adaptation of GH1 enzymes

The FEBS Journal, EarlyView.
Cold‐active enzymes retain high catalytic activity at low temperatures but are characterized by thermal instability, a behavior not fully understood. Comparison between a cold‐active GH1 and its mesophilic counterparts highlights the role of a three‐residue motif (W‐M‐F) in conferring structural stability to a mesophilic GH1.
Stefania Digiovanni, Gabriel Oanca, Marco Orlando, Marina Lotti, Johan Åqvist, Marco Mangiagalli   +5 more
wiley   +1 more source

Engineering of <i>Escherichia coli</i> for D-tagatose production from lactose and whey permeate via the tagatose-6-phosphate pathway. [PDF]

Curr Res Food Sci
Abzach A, Ben-Adiva R, Gruzdev N, Musa S, Yadid I.   +4 more
europepmc   +1 more source

Mechanistic insights into HapC: A key enzyme in prodiginine biosynthesis in Hahella chejuensis

The FEBS Journal, EarlyView.
HapC catalyzes prodiginine biosynthesis through coordinated movements of its ATP‐binding, substrate‐binding, and catalytic‐swivel domains. The enzyme shows tolerance toward short‐chain MAP analogs, and docking analyses reveal key residues and structural features underlying its substrate flexibility.
Yueh‐Te Chan, Aden Dhana Rizkita, Hsin‐Ju Hsieh, Shu‐Fang Hsu, Tony Eight Lin, Wei‐Jan Huang, Kai‐Cheng Hsu, Sheila Nathan, Peiching Chang, Andrew H.‐J. Wang, Cheng‐Chung Lee   +10 more
wiley   +1 more source

Optimized Bioconversion of Naringenin to Hesperetin in <i>Escherichia coli</i> With Halide Methyltransferase-Mediated <i>S</i>-Adenosylmethionine Regeneration. [PDF]

Eng Life Sci
Wildhagen M, Malke N, Wang Q, Zhuang X, Beutel S.   +4 more
europepmc   +1 more source

Functional characterization of 42 CK2α de novo variants associated with Okur‐Chung neurodevelopmental syndrome

The FEBS Journal, EarlyView.
Okur‐Chung neurodevelopmental syndrome (OCNDS) is a neurodevelopmental disorder associated with mutations in the gene coding for Protein kinase CK2α. In this work, 42 variants of CK2α associated with OCNDS were characterized in vitro. This included determination of catalytic activity and CK2α/CK2β‐interaction as well as an assessment of evolutionary ...
Alexander Gast, Christian Werner, Karsten Niefind, Joachim Jose   +3 more
wiley   +1 more source

Development of a recombinant biosimilar single-chain variable fragment antibody targeting human estrogen receptor α36. [PDF]

Res Pharm Sci
Shafiee S, Kolivand S, Jalili N, Abedini M, Navabi M, Talebkhan Y, Esmaeili R, Abdolvahab MH.   +7 more
europepmc   +1 more source

A distinct gene expression mechanism for d‐amino acid utilization in hyperthermophilic archaeon Pyrococcus horikoshii

The FEBS Journal, EarlyView.
The genes PH0137, PH0138, and PH0140 form a cluster in the genome of Pyrococcus horikoshii. The PH0140 gene encodes a transcription factor identified as a d‐amino acid‐responsive regulatory protein (DARP). In feast mode, l‐Ile‐bound DARP binds to the promoter and represses transcription of PH0137 and PH0138. In famine mode, DARP binds to d‐allo‐Ile and
Ryushi Kawakami, Tomoki Kawase, Taira Uehara, Haruhiko Sakuraba, Tomohiko Matsuzawa, Junji Hayashi, Toshihisa Ohshima   +6 more
wiley   +1 more source