Results 51 to 60 of about 97,366 (264)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Carbonation of Chrysotile under Subduction Conditions

Engineering, 2019
In order to understand the role of serpentine minerals in the global carbon cycle, high-pressure X-ray diffraction (XRD) experiments were performed on chrysotile (Mg3Si2O5(OH)4) using carbon dioxide (CO2) as a pressure medium.
Mihye Kong, Yongjae Lee
doaj   +1 more source

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

Pressure calibrations of high-pressure large-volume presses at HPSTAR [PDF]

Matter and Radiation at Extremes
Large-volume presses (LVPs) are widely utilized in diverse research fields—including high-pressure physics, chemistry, materials science, and Earth and planetary sciences—to investigate the physical and chemical properties of materials under extreme high-
Yongjiang Xu, 徐永江, Peiyan Wu, 吴培衍, Sheng Shang, 尚升, Xue Wang, 王雪, Taihang Li, 李钛航, Shuchang Gao, 高书畅, Shijie Lv, 吕世杰, Hang Cheng, 程行, Qianzhi Xu, 许潜智, Shang Lei, 雷尚, Jiajia Feng, 冯嘉嘉, Lei Zhao, 赵磊, Wim van Westrenen, Takayuki Ishii, 石井 貴之, Bin Chen, 陈斌, Lei Su, 苏磊, Yang Ding, 丁阳, Wenge Yang, 杨文革, Ho-Kwang Mao, 毛河光, Yanhao Lin, 林彦蒿   +38 more
doaj   +1 more source

Epigenetic heterogeneity and plasticity in therapy‐induced tumor states through single‐cell multi‐omics

Molecular Oncology, EarlyView.
Single‐cell multi‐omics reveals epigenetic heterogeneity across therapy‐adaptive tumor states, including quiescent/dormant, drug‐tolerant persister, and EMT‐like phenotypes. By linking regulatory features with state‐associated biomarkers, these approaches inform biomarker‐guided therapeutic strategies for evolving tumors.
Hee Jung Kim, Hwiyeong Lee, Jin Hong, Daechan Park   +3 more
wiley   +1 more source

A role for subducting clays in the water transportation into the Earth’s lower mantle

Nature Communications
Subducting sedimentary layer typically contains water and hydrated clay minerals. The stability of clay minerals under such hydrous subduction environment would therefore constraint the lithology and physical properties of the subducting slab interface ...
Yoonah Bang, Huijeong Hwang, Hanns-Peter Liermann, Duck Young Kim, Yu He, Tae-Yeol Jeon, Tae Joo Shin, Dongzhou Zhang, Dmitry Popov, Yongjae Lee   +9 more
doaj   +1 more source

PAK1 activation drives divergent resistance mechanisms to aromatase inhibition and tamoxifen in a luminal: A breast cancer model

Molecular Oncology, EarlyView.
Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller, Janina Müller, Efstathios‐Iason Vlachavas, Lukas Beumers, Lena Fleischhacker, Sara Burmester, Angelika Wörner, Sabine Karolus, Dominic Helm, Cindy Körner, Stefan Wiemann   +10 more
wiley   +1 more source

Asymmetrical deformation characteristics and mechanisms of deep excavations induced by one-side unloading

Yantu gongcheng xuebao
With the advancement of urban renewal, more and more excavations are being constructed under one-side unloading condition caused by the existing adjacent ones, resulting in different deformation characteristics and mechanisms compared to those of the ...
QIU Yanling 1, 2, DING Wenqi 1, 2, ZHAO Tengteng 3, WANG Xiaoyong 4, QIAO Yafei 1, 2
doaj   +1 more source

Real-Time Dynamic Earth-Pressure Regulation Model for Shield Tunneling by Integrating GRU Deep Learning Method With GA Optimization

IEEE Access, 2020
This paper proposes an intelligent framework to predict and automatically regulate earth pressure using a deep learning technique during earth pressure balance shield tunneling. A prediction model was proposed by integrating a new cost function (relative
Min-Yu Gao, Ning Zhang, Shui-Long Shen, Annan Zhou   +3 more
doaj   +1 more source