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A reappraisal of ecdysteroid binding in drosophila
Molecular and Cellular Endocrinology, 1983Ecdysteroid binding was investigated in Kc cells and larval fat body of Drosophila. After incubation of intact Kc cells, 200-300 specific binding sites were detected for both 20-OH ecdysone and ponasterone A. However, if cells were fractionated prior to incubation with 20-OH ecdysone, no specific binding was observed.
H, Gronemeyer, P, Harry, A, Alberga
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Ant Ecdysteroid Extraction and Radioimmunoassay
Cold Spring Harbor Protocols, 2009INTRODUCTIONEcdysteroids are a group of steroid compounds present in many plant and invertebrate species. In arthropods, they function primarily as hormones involved in the regulation of molting. This protocol describes how to extract ecdysteroid hormones from ant specimens and subsequently quantify circulating levels of the hormone. The hormone can be
Colin, Brent, Adam, Dolezal
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1984
The epidermal cells of most annelid worms secrete a cuticle which consists of a mixture of proteins and sugars. The Hirudinea have been reported, more than a century ago [1], to molt, a process which is less conspicuous in other annelid worms, although it is regularly observed in species like the polychaete Nereis divenicolor.
M. Porchet +4 more
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The epidermal cells of most annelid worms secrete a cuticle which consists of a mixture of proteins and sugars. The Hirudinea have been reported, more than a century ago [1], to molt, a process which is less conspicuous in other annelid worms, although it is regularly observed in species like the polychaete Nereis divenicolor.
M. Porchet +4 more
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Molecular and Cellular Endocrinology, 1988
A macromolecule with high affinity for the ecdysteroid analogue ponasterone A was isolated from nuclei of larvae of the blowfly Calliphora vicina. The ecdysteroid-binding molecule revealed characteristics of the moulting hormone receptor. It was sensitive towards protease but not towards nucleases.
M, Lehmann, J, Koolman
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A macromolecule with high affinity for the ecdysteroid analogue ponasterone A was isolated from nuclei of larvae of the blowfly Calliphora vicina. The ecdysteroid-binding molecule revealed characteristics of the moulting hormone receptor. It was sensitive towards protease but not towards nucleases.
M, Lehmann, J, Koolman
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Insect Biochemistry and Molecular Biology, 2002
Total body ecdysteroid titers were determined at specific stages during the larval and nymphal life of Amblyomma americanum (L.). One ecdysteroid peak was observed following the completion of larval apolysis. However, two distinct ecdysteroid peaks occurred at a comparable stage in the nymphal molting cycle.
Melanie J, Palmer +4 more
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Total body ecdysteroid titers were determined at specific stages during the larval and nymphal life of Amblyomma americanum (L.). One ecdysteroid peak was observed following the completion of larval apolysis. However, two distinct ecdysteroid peaks occurred at a comparable stage in the nymphal molting cycle.
Melanie J, Palmer +4 more
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ECDYSTEROIDS IN NATURE AND THEIR PROPERTIES
Clinical Medicine and Pharmacology, 2019Ecdysteroids are biologically active substances of natural origin. Their adaptogenic activity, hormone-like and bioregulatory effects used in traditional and folk medicine are known. Sources of raw materials are diverse. Applications depend on the methods of obtaining ecdysteroids and their natural sources.
B. Valentinov, E. Naumova
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Ecdysteroids: Isolation, Chemical Transformations, and Biological Activity
Phytochemistry Reviews, 2022Victor N Odinokov +2 more
exaly

